1997
DOI: 10.1677/jme.0.0190321
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Two complementary bioassays for screening the estrogenic potency of xenobiotics: recombinant yeast for trout estrogen receptor and trout hepatocyte cultures

Abstract: A relation between the chemical structure of a xenobiotic and its steroidal action has not yet been clearly established. Thus, it is not possible to define the estrogenic potency of different xenobiotics. An assessment may be accomplished by the use of different bioassays. We have previously developed a yeast system highly and stably expressing rainbow trout estrogen receptor (rtER) in order to analyze the biological activity of the receptor. The recombinant yeast system appears to be a reliable, rapid and sen… Show more

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Cited by 173 publications
(83 citation statements)
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“…The structure of PFCs is not similar to E2, but there may be other mechanisms by which these compounds elicit a response. Petit et al (1997) pointed out that certain xenobiotics may interact directly with the ER without disturbing E2 binding. Anti-estrogenic activities have also been observed by co-exposure of other chemicals with E2.…”
Section: Discussionmentioning
confidence: 99%
“…The structure of PFCs is not similar to E2, but there may be other mechanisms by which these compounds elicit a response. Petit et al (1997) pointed out that certain xenobiotics may interact directly with the ER without disturbing E2 binding. Anti-estrogenic activities have also been observed by co-exposure of other chemicals with E2.…”
Section: Discussionmentioning
confidence: 99%
“…For many years the mechanisms via which many xenoestrogens act remained a mystery. This lack of a mechanistic explanation existed because while these compounds can affect animal functions and development at relatively low concentrations, experimental systems for testing the classical nuclear transcriptional activities of xenoestrogens showed weak or no activity [3][4][5][6][7][8][9][10]. Therefore, the question remained, via what cellular mechanisms do xenoestrogens act?…”
Section: Introduction Xenoestrogens and Their Known Modes Of Actionmentioning
confidence: 99%
“…estrogen/androgen receptor transfected cell lines (e.g. Petit et al, 1997), receptor binding assays (e.g. Danzo, 1997) or in vivo assays in mammalians (e.g.…”
Section: Introductionmentioning
confidence: 99%