Two cytochrome P450 genes are involved in imidacloprid resistance in field populations of the whitefly, Bemisia tabaci, in China

Yang, Xin; Xie, Wen; Wang, Shaoli; Wu, Qingjun; Pan, Huipeng; Li, Rumei; Yang, Ning; Liu, Bai-ming; Xu, Baoyun; Zhou, Xiaomao; Zhang, Youjun

doi:10.1016/j.pestbp.2013.10.002

Cited by 100 publications

(84 citation statements)

References 35 publications

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“…Two of these ( P450 6B5-like and P450 9e2-like ) were selected for interrogation by qPCR, the results of which confirmed their specific expression in the K8 strain. Cytochrome P450 proteins function as a major class of detoxification enzymes [39], and most insecticide-resistant genes belong to the CYP4, CYP6 and CYP9 subfamilies [40,41,42]. Interestingly, two of the genes specifically expressed in K8 belong to the CYP6 and CYP9 classes, suggesting they may play roles in resistance to B. bassiana infection.…”

Section: Discussionmentioning

confidence: 99%

RNA-Seq Analyses for Two Silkworm Strains Reveals Insight into Their Susceptibility and Resistance to Beauveria bassiana Infection

Xing

Yang

Jiang

et al. 2017

IJMS

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The silkworm Bombyx mori is an economically important species. White muscardine caused by Beauveria bassiana is the main fungal disease in sericulture, and understanding the silkworm responses to B. bassiana infection is of particular interest. Herein, we investigated the molecular mechanisms underlying these responses in two silkworm strains Haoyue (HY, sensitive to B. bassiana) and Kang 8 (K8, resistant to B. bassiana) using an RNA-seq approach. For each strain, three biological replicates for immersion treatment, two replicates for injection treatment and three untreated controls were collected to generate 16 libraries for sequencing. Differentially expressed genes (DEGs) between treated samples and untreated controls, and between the two silkworm strains, were identified. DEGs and the enriched Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways of the two strains exhibited an obvious difference. Several genes encoding cuticle proteins, serine proteinase inhibitors (SPI) and antimicrobial peptides (AMP) and the drug metabolism pathway involved in toxin detoxification were considered to be related to the resistance of K8 to B. bassiana. These results revealed insight into the resistance and susceptibility of two silkworm strains against B. bassiana infection and provided a roadmap for silkworm molecular breeding to enhance its resistance to B. bassiana.

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Section: Discussionmentioning

confidence: 99%

RNA-Seq Analyses for Two Silkworm Strains Reveals Insight into Their Susceptibility and Resistance to Beauveria bassiana Infection

Xing

Yang

Jiang

et al. 2017

IJMS

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“…R esistance to DDT in Anopheles gambiae is related to Cyp6Z1 upregulated expression . Resistance to imidacloprid in B. tabaci is related to the overexpression of CYP6Z1 , and CYP4C64 is also linked to the resistance . In the COG database, all P450 family members have functional annotations of ‘second metabolites biosynthesis, transport, and catabolism’, consistent with P450s as major detoxification metabolic enzymes in insects .…”

Section: Discussionmentioning

confidence: 77%

“…Of 37 P450 transcripts from a B. tabaci population, most belonged to the CYP6 and CYP4 families . It has been reported that the CYP3, CYP4 and CYP6 family P450s are closely related to drug resistance in some insects . DDT medium resistance in Drosophila melanogaster is associated with overexpression of Cyp6g1 .…”

Section: Discussionmentioning

confidence: 99%

Detoxification enzymes associated with butene‐fipronil resistance in Epacromius coerulipes

Jin

Gao

Zhang

et al. 2019

Pest Management Science

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BACKGROUND Epacromius coerulipes is a widely distributed locust pest species. Chemical control is the main method used to kill locusts; however, this can result in the selection of locusts with resistance to chemical pesticides. Therefore, the study of resistance is of great significance for the sustainable management of locusts. RESULTS In this study, to investigate the relationship between detoxification enzymes and butene‐fipronil resistance in E. coerulipes, resistant strains of the locust were compared with sensitive strains. The synergism of synergistic agents was significantly enhanced, and the activities of multifunctional oxidase, carboxylesterase, and glutathione sulfur transferase were significantly increased. Transcriptome sequencing revealed 226 detoxification enzyme genes and 23 upregulated genes. Neighbor‐joining was used to construct a phylogenetic tree of related gene families, which included 59 P450 genes, 52 carboxylesterases (CarE) genes, and 25 glutathione S‐transferase (GST) genes. Reverse transcription polymerase chain reaction (RT‐PCR) analysis results of overexpressed genes in the resistant population combined with a phylogenetic tree showed that four P450 genes belonged to the CYP6, CYP4, CYP18 and CYP302 families, two CarE genes belonged to Clade A families, and one GST gene belonged to the Sigma family. These family members were annotated as detoxification enzyme genes of metabolic insecticide in the transcriptome databases. CONCLUSIONS This study showed that P450, CarE and GST together resulted in moderate resistance to butene‐fipronil in locusts. The analysis revealed several overexpressed detoxification enzyme genes that will be the focus of future studies on the mechanism of resistance to butene‐fipronil. © 2019 Society of Chemical Industry

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“…Furthermore, various genes belonging to these families have been reported to be associated with the detoxification of different types of insecticides in several insect pests. Examples include CYP6CM1 and CYP4C64, which are involved in imidacloprid resistance in field populations of Bemisia tabaci (Xin, ); CYP6AY1 upregulation in Nilaparvata lugens (Ding et al, ); CYP6CM1 overexpression in imidacloprid resistance in B. tabaci (Karunker et al, ); CYP6F1 in resistance to pyrethroids in Culex quinquefasciatus and Culex pipiens pallens (Gong et al, ); CYP6AE11 in deltamethrin‐resistant Helicoverpa armigera (Brun‐Barale et al, ); and five genes of the CYP4 family associated with imidacloprid resistance in Diaphorina citri (Tiwari et al, ). Accordingly, the upregulation of CYP4DE1 and CYP6CW3v2 was considered to be associated with ethiprole resistance in L. striatellus .…”

Section: Discussionmentioning

confidence: 99%

Cytochrome P450 CYP4DE1 and CYP6CW3v2 contribute to ethiprole resistance inLaodelphax striatellus(Fallén)

Elzaki

Zhang

Han

2015

Insect Molecular Biology

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Laodelphax striatellus Fallén (Hemiptera: Delphacidae), a destructive pest of rice, has developed high resistance to multiple insecticides, threatening the success of pest management programmes. The present study investigated ethiprole resistance mechanisms in a field population that is highly resistant to ethiprole. That population was used to establish a laboratory population that was subjected to further selection to produce a resistant strain. Target genes were cloned and compared between the resistant and the susceptible strains, the role of detoxification enzymes was examined, and the relative expression levels of 71 detoxification enzyme genes were tested using quantitative real time (RT)-PCR. The laboratory selection enhanced the resistance from 107-fold to 180-fold. The Rdl-type target site mutation seldom occurred in the resistant strain and is unlikely to represent the major mechanism underlying the observed resistance. Of the three important detoxification enzymes, only P450 monooxygenase was found to be associated with ethiprole resistance. Moreover, two genes, CYP4DE1 and CYP6CW3v2, were found to be overexpressed in the resistant strain. Furthermore, gene-silencing via a double-stranded RNA feeding test was carried out, and the results showed that the mRNA levels of CYP4DE1 and CYP6CW3v2 were reduced in the resistant strain, whereas ethiprole susceptibility was increased. These results suggest that CYP4DE1 and CYP6CW3v2 play an important role in ethiprole resistance in L. striatellus.

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Two cytochrome P450 genes are involved in imidacloprid resistance in field populations of the whitefly, Bemisia tabaci, in China

Cited by 100 publications

References 35 publications

RNA-Seq Analyses for Two Silkworm Strains Reveals Insight into Their Susceptibility and Resistance to Beauveria bassiana Infection

RNA-Seq Analyses for Two Silkworm Strains Reveals Insight into Their Susceptibility and Resistance to Beauveria bassiana Infection

Detoxification enzymes associated with butene‐fipronil resistance in Epacromius coerulipes

Cytochrome P450 CYP4DE1 and CYP6CW3v2 contribute to ethiprole resistance inLaodelphax striatellus(Fallén)

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