Two cytosolic aldolases show different expression patterns during shoot elongation in Moso bamboo, Phyllostachys pubescens Mazel

Abstract

In fast-growing Moso bamboo (Phyllostachys pubescens Mazel), cytosolic fructose 1,6-bisphosphate aldolase (aldolase; EC 4.2.2.13) was more highly active in elongating tissues than in tissues that had already finished elongating. It is well known that the removal of the culm sheath prevents bamboo from elongating. When the sheath was removed from the culm, the aldolase activity was gradually reduced over time. Two isozyme genes for aldolase, PpAldC1 and PpAldC2, were cloned from the elongating tissues of Moso bamboo. Gene expression analysis using a semi-quantitative reverse transcriptase-polymerase chain reaction revealed that PpAldC1 was highly expressed in elongating tissues but was hardly detected in elongated internodes, while PpAldC2 seemed to be expressed constitutively in both elongating and elongated tissues. Promoter analysis revealed that the expression of PpAldC1 was induced by gibberellin. These results indicated that the two genes for cytosolic aldolase in Moso bamboo showed different expression patterns and that one of them was involved in shoot elongation.

Shifts in diversity and community structure of endophytic bacteria and archaea across root, stem and leaf tissues in the common reed, Phragmites australis, along a salinity gradient in a marine tidal wetland of northern China

“…However, there was also a high abundance of aldolase in the rhizosphere soils at 4-5 mm from the root surface where the soil is anaerobic. Lao et al (2013) reported that aldolase induced under these conditions, and its activity rapidly decreased following the transition of plants to aerobic conditions. The anaerobic biodegradation of PAHs is a slow process, and the biochemical mechanisms involved have not yet been fully elucidated (Haritash and Kaushik 2009).…”

Reconstructed metagenomes reveal changes of microbial functional profiling during PAHs degradation along a rice (Oryza sativa) rhizosphere gradient

“…Lao et al . () reported that aldolase induced under these conditions, and its activity rapidly decreased following the transition of plants to aerobic conditions. The anaerobic biodegradation of PAHs is a slow process, and the biochemical mechanisms involved have not yet been fully elucidated (Haritash and Kaushik ).…”

Identification of a peroxisomal-targeted aldolase involved in chlorophyll biosynthesis and sugar metabolism in rice

“…Similarly, the expression levels of chloroplast development-related genes Cab1R and Cab2R, which encode two light-harvesting Chl a/b-binding proteins of PSII [27], and psaA and psbA, which separately encode two reaction center polypeptides of PSI and PSII, were also significantly down-regulated (Fig. 3A).…”