Two-Dimensional and 3-Dimensional Analysis of Bone/Dental Implant Interfaces With the Use of Focused Ion Beam and Electron Microscopy

Giannuzzi, Lucille A.; Phifer, Daniel; Giannuzzi, Nicholas J.; Capuano, Mario J.

doi:10.1016/j.joms.2006.10.025

Cited by 79 publications

(63 citation statements)

References 7 publications

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“…FIB-SEM sectioning was shown to be appropriate for biological samples for the first time in 1993 (Young et al, 1993). Since this seminal study this method has improved and several groups have reported about the use of FIB-SEM microscopes for material sciences and biological materials (Phaneuf, 1999;Uchic et al, 2006;Giannuzzi et al, 2007;Leser et al, 2009;Grandfield and Engquvist, 2012;Srot et al, 2012). Nevertheless, the investigation of biological and non-conductive materials remains challenging due to the radiation-sensitive nature of these samples and their interaction with the electron and ion beam (Grandfield and Engquvist, 2012).…”

Section: Discussionmentioning

confidence: 99%

“…Using FIB-SEM tomography to investigate insulators like biological, geological and ceramic samples is challenging because of charging effects that disturb the sectioning as well as the imaging (De Winter et al, 2009). Nevertheless, the FIB-SEM microscope is often used to analyse biological materials which are difficult to cut, such as teeth (Nalla et al, 2005) and bones (Giannuzzi et al, 2007). Another application for FIB-SEM microscopes in biology is the preparation of thin lamellae which can be analysed in a transmission electron microscope (TEM) (De Winter et al, 2009;Schmahl et al, 2008;Kelm et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

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Insight into <i>Emiliania huxleyi</i> coccospheres by focused ion beam sectioning

et al. 2015

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Abstract. Coccospheres of a cultured Emiliania huxleyi clone were sampled in the exponential growth phase and sectioned using a focused ion beam microscope. An average of 69 sections and the corresponding secondary electron micrographs per coccosphere provided detailed information on coccosphere architecture. The coccospheres feature 2-3 layers on average and 20 coccoliths per cell, of which only 15 can be seen in conventional scanning electron micrographs. The outer coccosphere diameter was positively correlated with the number of coccolith layers. By contrast, the inner coccosphere diameter (around 4.36 µm), and hence the cell diameter, was quasi-constant. Coccoliths were not evenly distributed across the coccosphere, resulting more often than not in one part of the coccosphere displaying more coccolith layers than the other. The architectural data allowed for the calculation of the PIC / POC ratio, the density and the sinking velocity of individual cells. The correlation of these parameters has implications for the ongoing debate on the function of coccoliths.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Insight into <i>Emiliania huxleyi</i> coccospheres by focused ion beam sectioning

et al. 2015

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“…However, there are only a small number of examples in the literature where these methods have been applied to biomineralization, including the study of mollusc sclerites (Suzuki et al, 2006) and shell (Valazquez-Castillo et al, 2006). FIB has also been applied to the study of interfaces between biological and innate surfaces in biomedical applications (Engqvist et al, 2006;Giannuzzi et al, 2007;Engqvist et al, 2008).…”

Section: Accepted Manuscriptmentioning

confidence: 99%

Characterization of biominerals in the radula teeth of the chiton, Acanthopleura hirtosa

Saunders

Kong

Shaw

et al. 2009

Journal of Structural Biology

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ABSTRACT:Understanding biomineralization processes provides a route to the formation of novel biomimetic materials with potential applications in fields from medicine to materials engineering. The teeth of chitons (marine molluscs) represent an excellent example of a composite biomineralized structure, comprising variable layers of iron oxide, iron oxyhydroxide and apatite. Previous studies of fully mineralized teeth using X-ray diffraction, Raman spectroscopy and scanning electron microscopy (SEM) have hinted at the underlying microstructure, but have lacked the resolution to provide vital information on fine scale structure, particularly at interfaces. While transmission electron microscopy (TEM) is capable of providing this information, difficulties in producing suitable samples from the hard, complex biocomposite have hindered progress. To overcome this problem we have used focused ion beam (FIB) processing to prepare precisely oriented sections across interfaces in fully mineralized teeth. In particular, the composite structure is found to be more complex than previously reported, with additional phases (goethite and amorphous apatite) and interface detail observed. This combination of FIB processing and TEM analysis has enabled us to investigate the structural and compositional properties of this complex biocomposite at higher resolution than previously reported and has the potential to significantly enhance future studies of biomineralisation in these animals.

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“…Recently, focused ion beam (FIB) milling was used to prepare cross sections of dental implants and the tissue/implant interfaces. It has been proven a promising method for preparing cross sections without mechanical impact on the sample for electron microscope characterization, particularly for transmission electron microscopy (TEM) observation [8][9][10][11]. The limitation of FIB is the fact that the polished area is rather small, typical about 20 μm in edge lengths, which only cover one or two pores in case of the oxidized titanium implants.…”

Section: Introductionmentioning

confidence: 99%

Microscopic view of osseointegration and functional mechanisms of implant surfaces

Han

Shen

2015

Materials Science and Engineering: C

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Argon ion beam polishing technique was applied to prepare the cross sections of implants feasible for high resolution scanning electron microscope investigation. The interfacial microstructure between newly formed bone and implants with three modified surfaces retrieved after in vivo test using three different animal models was characterized. By this approach it has become possible to directly observe early bone formation, the increase of bone density, and the evolution of bone structure. The two bone growth mechanisms, distant osteogenesis and contact osteogenesis, can also be distinguished. These direct observations give, at microscopic level, a better view of osseointegration and expound the functional mechanisms of various implant surfaces for osseointegration.

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Two-Dimensional and 3-Dimensional Analysis of Bone/Dental Implant Interfaces With the Use of Focused Ion Beam and Electron Microscopy

Cited by 79 publications

References 7 publications

Insight into <i>Emiliania huxleyi</i> coccospheres by focused ion beam sectioning

Insight into <i>Emiliania huxleyi</i> coccospheres by focused ion beam sectioning

Characterization of biominerals in the radula teeth of the chiton, Acanthopleura hirtosa

Microscopic view of osseointegration and functional mechanisms of implant surfaces

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Two-Dimensional and 3-Dimensional Analysis of Bone/Dental Implant Interfaces With the Use of Focused Ion Beam and Electron Microscopy

Cited by 79 publications

References 7 publications

Insight into &lt;i&gt;Emiliania huxleyi&lt;/i&gt; coccospheres by focused ion beam sectioning

Insight into &lt;i&gt;Emiliania huxleyi&lt;/i&gt; coccospheres by focused ion beam sectioning

Characterization of biominerals in the radula teeth of the chiton, Acanthopleura hirtosa

Microscopic view of osseointegration and functional mechanisms of implant surfaces

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Insight into <i>Emiliania huxleyi</i> coccospheres by focused ion beam sectioning

Insight into <i>Emiliania huxleyi</i> coccospheres by focused ion beam sectioning