Two-dimensional electrophoresis of soluble leaf proteins, isolated from two wheat species (Triticum durum andTriticum aestivum) differing in sensitivity towards NaCl

Ouerghi, Zeineb; Rémy, R.; Ouelhazi, Lazhar; Ayadi, A.; Brulfert, J.

doi:10.1002/1522-2683(20000701)21:12<2487::aid-elps2487>3.0.co;2-b

Cited by 34 publications

(2 citation statements)

References 25 publications

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“…The experiment was carried on durum wheat (Triticum durum) of the Cirta R1 variety, with susceptibilities to abiotic stresses comparable to other Triticum durum parental genotypes [45][46][47]. To obtain optimal germination rate, the seeds were kept in sterile distilled water for 24 h. The seeds were surface sterilized with a 20% (v/v) sodium hypochlorite solution washed several times with sterile distilled water [5] and left to dry in sterile conditions for 3 h. The inoculation was carried out by immersing the seeds in spore suspensions (10 6 spores mL −1 ) of the different isolates for 1 h with moderate agitation; seeds dipped in sterile distilled water were used as control (uninoculated) [30].…”

Section: Greenhouse Experiments On Triticum Durummentioning

confidence: 99%

Characterization of Plant Growth-Promoting Traits and Inoculation Effects on Triticum durum of Actinomycetes Isolates under Salt Stress Conditions

et al. 2021

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This study aimed to characterize the halotolerant capability, in vitro, of selected actinomycetes strains and to evaluate their competence in promoting halo stress tolerance in durum wheat in a greenhouse experiment. Fourteen isolates were tested for phosphate solubilization, indole acetic acid, hydrocyanic acid, and ammonia production under different salt concentrations (i.e., 0, 0.25, 0.5, 0.75, 1, 1.25, and 1.5 M NaCl). The presence of 1-aminocyclopropane-1-carboxylate deaminase activity was also investigated. Salinity tolerance was evaluated in durum wheat through plant growth and development parameters: shoot and root length, dry and ash-free dry weight, and the total chlorophyll content, as well as proline accumulation. In vitro assays have shown that the strains can solubilize inorganic phosphate and produce indole acetic acid, hydrocyanic acid, and ammonia under different salt concentrations. Most of the strains (86%) had 1-aminocyclopropane-1-carboxylate deaminase activity, with significant amounts of α-ketobutyric acid. In the greenhouse experiment, inoculation with actinomycetes strains improved the morpho-biochemical parameters of durum wheat plants, which also recorded significantly higher content of chlorophylls and proline than those uninoculated, both under normal and stressed conditions. Our results suggest that inoculation of halotolerant actinomycetes can mitigate the negative effects of salt stress and allow normal growth and development of durum wheat plants.

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Section: Greenhouse Experiments On Triticum Durummentioning

confidence: 99%

Characterization of Plant Growth-Promoting Traits and Inoculation Effects on Triticum durum of Actinomycetes Isolates under Salt Stress Conditions

et al. 2021

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“…In the context of drought stress, Krugman et al [34] compared resistant and susceptible wild emmer genotypes to investigate the overlapping regulatory and signaling processes overrepresented in the drought-resistant genotype and to identify candidate genes for drought tolerance. Proteomics has also been used to investigate the response of wheat to nitrogen [35], salinity stress [36,37], low temperatures [38] and drought [39,40]. Poor correlation between transcriptomic and proteomic data has been reported in some stress experiments although such complementary information highlights the multilevel regulation of responses to complex environments [41,42].…”

Section: Introductionmentioning

confidence: 99%

Transcriptomic and proteomic analyses of a pale-green durum wheat mutant shows variations in photosystem components and metabolic deficiencies under drought stress

Peremartí

Marè²,

Aprile

et al. 2014

BMC Genomics

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BackgroundLeaf pigment content is an important trait involved in environmental interactions. In order to determine its impact on drought tolerance in wheat, we characterized a pale-green durum wheat mutant (Triticum turgidum L. var. durum) under contrasting water availability conditions.ResultsThe pale-green mutant was investigated by comparing pigment content and gene/protein expression profiles to wild-type plants at anthesis. Under well-watered (control) conditions the mutant had lower levels of chlorophylls and carotenoids, but higher levels of xanthophyll de-epoxidation compared to wild-type. Transcriptomic analysis under control conditions showed that defense genes (encoding e.g. pathogenesis-related proteins, peroxidases and chitinases) were upregulated in the mutant, suggesting the presence of mild oxidative stress that was compensated without altering the net rate of photosynthesis. Transcriptomic analysis under terminal water stress conditions, revealed the modulation of antioxidant enzymes, photosystem components, and enzymes representing carbohydrate metabolism and the tricarboxylic acid cycle, indicating that the mutant was exposed to greater oxidative stress than the wild-type plants, but had a limited capacity to respond. We also compared the two genotypes under irrigated and rain-fed field conditions over three years, finding that the greater oxidative stress and corresponding molecular changes in the pale-green mutant were associated to a yield reduction.ConclusionsThis study provides insight on the effect of pigment content in the molecular response to drought. Identified genes differentially expressed under terminal water stress may be valuable for further studies addressing drought resistance in wheat.

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Plant Proteomics

Saravanan¹,

Bashir²,

Rose³

2004

Handbook of Plant Biotechnology

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Proteomics, or the study of the protein complement of the genome, promises to help bridge the divide between genomic DNA sequence and biological state and represents one of the most rapidly developing and innovative fields in genome‐scale research. The term is now commonly and broadly used to encompass almost any aspect of protein structure, expression and function. However, as used here it refers to characterising quantitative and qualitative characteristics of global protein expression, with an emphasis on scale, including polypeptide synthesis, degradation, post‐translational modification, the structure of protein complexes and interactions with other proteins and cellular components. The systematic characterisation of complex protein populations can be pursued using many approaches and numerous reviews have recently been published describing developments in diverse areas of proteomics, including protein structure, function and protein–protein, or protein–ligand interactions A number of reviews have also been specifically devoted to plant proteomics. Consequently, the multiplicity of proteomics‐related experimentation and developing technologies are not reiterated here in detail. Instead, this chapter intends to provide an overview of the diversity of proteomics‐based studies that have been reported in plants to date and emerging technologies, which in turn should underscore the tremendous potential utility of proteomics for plant biologists and give an indication of future applications.

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Two-dimensional electrophoresis of soluble leaf proteins, isolated from two wheat species (Triticum durum andTriticum aestivum) differing in sensitivity towards NaCl

Cited by 34 publications

References 25 publications

Characterization of Plant Growth-Promoting Traits and Inoculation Effects on Triticum durum of Actinomycetes Isolates under Salt Stress Conditions

Characterization of Plant Growth-Promoting Traits and Inoculation Effects on Triticum durum of Actinomycetes Isolates under Salt Stress Conditions

Transcriptomic and proteomic analyses of a pale-green durum wheat mutant shows variations in photosystem components and metabolic deficiencies under drought stress

Plant Proteomics

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