Two-dimensional gel electrophoresis of chicken skeletal muscle proteins with agarose gels in the first dimension

Hirabayashi, Tamio

doi:10.1016/0003-2697(81)90804-6

Cited by 119 publications

(37 citation statements)

References 9 publications

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“…Western blotting was performed using the GDH-antibody (1:100, Rockland Inc.) as the primary antibody and goat TRITC-conjugated anti-rabbit IgG (H+L) (1:100, Jackson ImmunoResearch) as the second antibody. Twodimensional gel electrophoresis (2D-PAGE) was performed by the Hirabayashi's procedure (Hirabayashi, 1981). For semi-nondenaturing 2D-PAGE, 2D-PAGE was performed with the decreased urea concentration (2 M instead of 5 M) for preparing samples and agarose-IEF gels in the first dimension (Hirabayashi, 1981).…”

Section: Electrophoresis and Western Blottingmentioning

confidence: 99%

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Interaction of Chicken Liver Tropomyosin with Glutamate Dehydrogenase

et al. 2000

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Section: Electrophoresis and Western Blottingmentioning

confidence: 99%

“…Twodimensional gel electrophoresis (2D-PAGE) was performed by the Hirabayashi's procedure (Hirabayashi, 1981). For semi-nondenaturing 2D-PAGE, 2D-PAGE was performed with the decreased urea concentration (2 M instead of 5 M) for preparing samples and agarose-IEF gels in the first dimension (Hirabayashi, 1981).…”

Section: Electrophoresis and Western Blottingmentioning

confidence: 99%

Interaction of Chicken Liver Tropomyosin with Glutamate Dehydrogenase

et al. 2000

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“…(Laemmli, 1970). Two-dimensional electrophoresis (2-D PAGE) combined with agarose (GE Healthcare) isoelectric focusing (agarose IEF) was carried out according to the method of Hirabayashi with some modifications (Hirabayashi, 1981). Preblended Ampholine (pH3.5-9.5) was used as a carrier ampholite.…”

Section: Cross-linkage Experimentsmentioning

confidence: 99%

Involvement of redox- and phosphorylation-dependent pathways in osmotic adaptation in sperm cells of euryhaline tilapia

Morita

Nakajima

Takemura

et al. 2011

Journal of Experimental Biology

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SUMMARYSperm cells involved in fertilisation must tolerate hypo-osmotic and hyper-osmotic environments. Euryhaline tilapia (Oreochromis mossambicus) can acclimatise to and reproduce in freshwater and seawater because its sperm are able to adapt to these differing osmotic environments. In this study, we found that the dephosphorylation of sperm proteins in O. mossambicus correlated with the activation of flagellar motility when sperm were exposed to hypotonic or hypertonic conditions, and that differences in phosphorylation may reflect adaptations to a given osmotic environment. Of the sperm proteins that were dephosphorylated, the phosphorylation pattern of an 18kDa protein, identified as the superoxide anion scavenger Cu/Zn superoxide dismutase (Cu/Zn SOD), was different in freshwater-and seawater-acclimatised tilapia sperm. Cu/Zn SOD was distributed from the sperm head to the flagellum. Additionally, differences were observed between freshwater and seawater tilapia in the nitration of tyrosine residues (which might be mediated by SOD) in sperm flagellar proteins in response to osmotic shock. These results demonstrate that reactive-oxygen-species-dependent mechanisms contribute to both osmotic tolerance and the activation of flagellar motility.

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“…9 The trick to analyzing high molecular mass protein lies in the use of agarose gel instead of polyacrylamide gel for the first-dimensional IEF. This method was initially described by Hirabayashi in 1981; 18 we have continued to develop it thereafter. Details of the procedure are described in our earlier reports.…”

Section: Proteome Of Prostate Cancer H Kuruma Et Almentioning

confidence: 99%

Proteome analysis of prostate cancer

Kuruma

Egawa

Ohishi

et al. 2004

Prostate Cancer Prostatic Dis

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In this paper, we briefly review cancer proteomics in general, with particular attention to our proteome analyses of prostate cancer. Our efforts include development of new tools and novel approaches to discovering proteins potentially useful as cancer diagnostic and/or prognostic biomarkers or as therapeutic targets. To this end, we analyzed prostate cancer proteomes using twodimensional gel electrophoresis employing agarose gels for the initial isoelectric focusing step (agarose 2-DE), with mass spectrometry used for protein identification. Agarose 2-DE offers advantages over the more widely used immobilized pH gradient 2-DE for separating high molecular mass proteins (15-500 kDa), thereby increasing its power to detect changes in the cancer's high-molecular mass proteomes.

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Two-dimensional gel electrophoresis of chicken skeletal muscle proteins with agarose gels in the first dimension

Cited by 119 publications

References 9 publications

Interaction of Chicken Liver Tropomyosin with Glutamate Dehydrogenase

Interaction of Chicken Liver Tropomyosin with Glutamate Dehydrogenase

Involvement of redox- and phosphorylation-dependent pathways in osmotic adaptation in sperm cells of euryhaline tilapia

Proteome analysis of prostate cancer

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