Two-dimensional high performance liquid chromatography for the separation of complex mixtures of explosives and their by-products

Köhne, Anja P.; Dornberger, Uwe; Welsch, Thomas

doi:10.1007/bf02467508

Cited by 39 publications

(24 citation statements)

References 34 publications

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“…Synthetic polymers [24], peptide fragments, or other biopolymers [25 -28] are often separated using size exclusion chromatography for separation according to the molar mass distribution in one dimension and reversed-phase [24 -26] or ion-exchange [28] LC for separation of species with different functionalities in the second one [28,29]. For separation of biopolymers, bioaffinity chromatography in the first dimension can be combined with reversed-phase LC in the second dimension [27].…”

Section: Introductionmentioning

confidence: 99%

Development of different comprehensive two dimensional systems for the separation of phenolic antioxidants

Cacciola

Jandera²,

Blahová

et al. 2006

J of Separation Science

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Three different comprehensive 2-D HPLC systems for the separation of phenolic antioxidants have been developed on the basis of different selectivities of a PEG-silica column in the first dimension and a packed or monolithic C18 or a ZR-CARBON column, respectively, in the second dimension. Two-dimensional comprehensive liquid chromatography using a serially connected short PEG-silica column and a conventional C18-silica or a ZR-CARBON column in the second dimension was tested to improve the resolution of the earlier eluting compounds in the first dimension. Various types of interface were used to connect the columns in the first and in the second dimension: i) two injection sampling loops of 100 microL in conventional arrangement; ii) a 10-port 2-position valve equipped with two trapping X-Terra columns instead of loops; and iii) two analytical D2 columns in parallel. The mobile phase in the first dimension has a lower elution strength than in the second dimension, allowing band compression of the solutes transferred from the first to the second dimension. This effect was enhanced using trapping columns instead of sampling loops as the interface between the two dimensions, thus allowing a decrease in the time of analysis. These systems were used for the analysis of beer samples. The relative location of the components in the 2-D retention plane varied in relation to their chemical structure in each instrumental set-up and allowed positive peak identification.

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Section: Introductionmentioning

confidence: 99%

Development of different comprehensive two dimensional systems for the separation of phenolic antioxidants

Cacciola

Jandera²,

Blahová

et al. 2006

J of Separation Science

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show abstract

“…4) or three [117] 6-port valves, which both accomplished complete isolation of the primary column during analysis of the previously collected fraction in the second dimension. No peak broadening effect was observed caused by the flow interruption in the first dimension.…”

Section: Stop-flow Interfacementioning

confidence: 99%

Comprehensive liquid chromatography: Fundamental aspects and practical considerations—A review

François

Sandra

2009

Analytica Chimica Acta

227

150

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“…Some aminophenols, chlorophenols, nitrophenols, and nitro-and nitroamino aromatic compounds used as explosives and their degradation products were separated using comprehensive 2-D LC6LC chromatography with a safrol-modified silica [151] or a tetrachlorophthalimidopropyl silica [35] in the first dimension, providing specific charge-transfer interactions with sample compounds, and a short nonporous small-particle alkylsilica column providing fast separation in the second dimension. A comprehensive LC6LC system using an affinity chromatography column with HSA bonded on silica gel providing specific interactions in the first dimension and a monolithic C18 column in the second dimension was used for the analysis of natural extracts containing traditional Chinese medicines [152].…”

Section: Rp6rp and Rp6np 2-d Separation Of Antioxidants Natural Andmentioning

confidence: 99%

Column selectivity for two‐dimensional liquid chromatography

Jandera¹

2006

J of Separation Science

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Selectivity of phase system is of primary concern when designing a 2-D separation, as it affects the 2-D system orthogonality and consequently the peak capacity controlling the number of peaks that can be separated in the available 2-D retention space limited by the time of analysis. Possibilities for characterization of LC phase system selectivity with respect to different polar and nonpolar structural units are compared, with special attention to multidimensional samples with various types of repeat groups, such as homopolymers, (co)polymers, fatty acid esters with various acyl lengths and number and position of double bonds, etc. Possibilities of the 2-D LC separations of these and other sample types, including pharmaceuticals, natural phenolic compounds, biopolymers, etc., using various combinations of separation modes are reviewed. Rules for design of comprehensive 2-D LC x LC systems are discussed, with respect to mobile phase compatibility in the two systems and modulation techniques suppressing band broadening connected with the sample fraction transfer from the first to the second dimension. Pitfalls connected with online connection of normal-phase and RP LC systems and their possible practical solutions are addressed and illustrated by practical examples.

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Two-dimensional high performance liquid chromatography for the separation of complex mixtures of explosives and their by-products

Cited by 39 publications

References 34 publications

Development of different comprehensive two dimensional systems for the separation of phenolic antioxidants

Development of different comprehensive two dimensional systems for the separation of phenolic antioxidants

Comprehensive liquid chromatography: Fundamental aspects and practical considerations—A review

Column selectivity for two‐dimensional liquid chromatography

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