Two DNA sequences specific for the canine Y chromosome

Abstract

Data are presented on the characterization of two nucleotide sequences found exclusively in the DNA of male dogs. In polymerase chain reactions (PCRs) of canine genomic DNA with a decanucleotide primer of arbitrary sequence (OP-W17), two nucleotide segments (650 and 990 bp) were amplified only from male samples, whereas a number of other fragments between 400 and 2500 bp in size were amplified from both male and female samples. The two male-specific segments were cloned and sequenced, and terminal 24mer oligonucleotide primer pairs were synthesized. PCR with these specific primer pairs resulted in amplification of the two male-specific sequences only from DNA samples of 34 male dogs; no product was amplified from 42 samples of females. A segment of the SRY gene previously localized on the Y chromosome could be amplified in DNA samples that had the two new sequences. EcoRI digested genomic male DNA when hybridized with the 650 bp or the 990 bp sequences, resulted in a single band for each on Southern analysis; DNA from females did not yield any bands. Comparisons between the two new sequences and the SRY gene segment revealed no homologies. We concluded that the two new sequences are specific for the canine Y chromosome and do not contain the short characterized segment of the SRY gene.

Canine Follicle Stem Cell Candidates Reside in the Bulge and Share Characteristic Features with Human Bulge Cells

“…Interestingly, about 40% of regenerated hair follicles were larger than other reconstituted mouse-size hair follicles, although they were much smaller than canine primary hair follicles (Figures 5f and g). PCR analysis successfully detected a canine-specific Y chromosome DNA sequence (Olivier and Lust, 1998) in the regenerated tissue, suggesting that it was of canine origin ( Figure 6a) More importantly, canine mRNA of the bulge markers K15, CD200 and follistatin, as well as a sebocyte lineage marker, peroxisome proliferator-activated receptor gamma (PPARG) (Tontonoz et al, 1995) were detected in reconstituted pilosebaceous structures ( Figure 6b), suggesting that canine bulge cells contributed to the reorganization of the bulge region and sebaceous glands. These findings provided supporting evidence for the multipotency of canine bulge cells.…”

A case of canine chimerism diagnosed using coat color tests

“…Absence of a heat cycle at ten months of age is not unusual for dogs of this breed. Y chromosome primers from Olivier and Lust [4] failed to detect the presence of the Y chromosome in any of the three DNA samples from this female pup (Table 1).…”

Y-chromosome DNA Is Present in the Blood of Female Dogs Suggesting the Presence of Fetal Microchimerism

“…The primers were designed based on the published sequences of canine male-specific DNA fragments originally detected in Labrador Retrievers [26]. Two sets of primers were synthesized; one set of primers for a 650 bp fragment, and a second set of nested primers within the 650 DNA fragment (∼320 bp fragment).…”