2021
DOI: 10.3390/molecules26040904
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Two Ecdysteroids Isolated from Micropropagated Lychnis flos-cuculi and the Biological Activity of Plant Material

Abstract: Genetically uniform plant material, derived from Lychnis flos-cuculi propagated in vitro, was used for the isolation of 20-hydroxyecdysone and polypodine B and subjected to an evaluation of the antifungal and antiamoebic activity. The activity of 80% aqueous methanolic extracts, their fractions, and isolated ecdysteroids were studied against pathogenic Acanthamoeba castellani. Additionally, a Microtox® acute toxicity assay was performed. It was found that an 80% methanolic fraction of root extract exerts the m… Show more

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Cited by 16 publications
(10 citation statements)
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“…Microtox has been tested for its use as an assessment of the toxicity of soils and polluted waters. Still, because the organisms used in the test are Gram-negative bacteria, it can also be used for the initial evaluation of the antimicrobial potential of chemical compounds, including the biopolymeric system [ 89 ]. The results of the study are presented in Figure 11 .…”
Section: Resultsmentioning
confidence: 99%
“…Microtox has been tested for its use as an assessment of the toxicity of soils and polluted waters. Still, because the organisms used in the test are Gram-negative bacteria, it can also be used for the initial evaluation of the antimicrobial potential of chemical compounds, including the biopolymeric system [ 89 ]. The results of the study are presented in Figure 11 .…”
Section: Resultsmentioning
confidence: 99%
“…Plants produce phytoecdysteroids as secondary metabolites to protect themselves from phytophagous insects and other pathogens [51][52][53].…”
Section: Discussionmentioning
confidence: 99%
“…[M+Na] + (cal for C27H44O8Na + , 519.2928); 1 H-NMR (CD3OD, 600 MHz) and 13 C-NMR (CD3OD, 150 MHz). Observed data was compared with literature and compound was identified as polypodine B [Maliński et al, 2021].…”
Section: Polypodine B (7)mentioning
confidence: 99%
“…S37-S41), protodioscin (5) [Abdel-Sattar et al, 2008] (Fig. S42-S46), 20β-hydroxyecdysone ( 6) [Maliński et al, 2021] (Fig. S47-S51), and polypodine B ( 7) [Malińskiet al, 2021] (Fig.…”
Section: Isolation and Characterization Of Phytomoleculesunclassified
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