Two endemic Onosma species (O. sieheana and O. stenoloba): A comparative study including docking data on biological activity and phenolic composition

Sarıkürkçü, Cengiz; Sahinler, Saliha Seyma; Hüsunet, Mehmet Tahir; İstifli, Erman Salih; Tepe, Bektaş

doi:10.1016/j.indcrop.2020.112656

Cited by 18 publications

(31 citation statements)

References 59 publications

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“…In previous studies, it was reported that some Onosma and Anthemis species were to be rich in apigenin 7-glucoside showing significant antioxidant activity [ 5 , 26 , 29 , 30 ]. There were also some studies in the literature published by other research groups that this compound may positively affect antioxidant activity.…”

Section: Discussionmentioning

confidence: 99%

“…Many Onosma species have been scrutinized to determine their biologically active substances such as deoxyshikonin, acetyl shikonin, 3-hydroxyisovaleryl shikonin and 5,8-O-dimethylacetyl shikonin [ 2 ]. Shikonin derivatives, known to be produced by some members of Onosma , are used as natural dyes due to their red color and have also been used in the silk and food industries since ancient times [ 3 , 4 , 5 ]. The cultivation of Onosma species, therefore, is of great importance.…”

Section: Introductionmentioning

confidence: 99%

“…However, the amount of this substance can decrease dramatically, especially in the elderly individuals, due to the hydrolysis by cholinesterases (ChEs), which are responsible for the regulation of the amount of ACh [ 22 ]. Therefore, inhibition of ChEs is a useful strategy in these individuals in order to prevent the decrease in the amount of neurotransmitters and to stabilize cognitive functions [ 5 , 23 , 24 , 25 , 26 , 27 , 28 ].…”

Section: Introductionmentioning

confidence: 99%

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Chemical Composition, Antioxidant and Enzyme Inhibitory Activities of Onosma bourgaei and Onosma trachytricha and in Silico Molecular Docking Analysis of Dominant Compounds

İstifli

2021

Molecules

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The aim of this study was to investigate the chemical composition, antioxidant and enzyme inhibitory activities of methanol (MeOH) extracts from Onosma bourgaei (Boiss.) and O. trachytricha (Boiss.). In addition, the interactions between phytochemicals found in extracts in high amounts and the target enzymes in question were revealed at the molecular scale by performing in silico molecular docking simulations. While the total amount of flavonoid compounds was higher in O. bourgaei, O. trachytricha was richer in phenolics. Chromatographic analysis showed that the major compounds of the extracts were luteolin 7-glucoside, apigenin 7-glucoside and rosmarinic acid. With the exception of the ferrous ion chelating assay, O. trachytricha exhibited higher antioxidant activity than O. bourgaei. O. bourgaei exhibited also slightly higher activity on digestive enzymes. The inhibitory activities of the Onosma species on tyrosinase were almost equal. In addition, the inhibitory activities of the extracts on acetylcholinesterase (AChE) were stronger than the activity on butyrylcholinesterase (BChE). Molecular docking simulations revealed that luteolin 7-glucoside and apigenin 7-glucoside have particularly strong binding affinities against ChEs, tyrosinase, α-amylase and α-glucosidase when compared with co-crystallized inhibitors. Therefore, it was concluded that the compounds in question could act as effective inhibitors on cholinesterases, tyrosinase and digestive enzymes.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Chemical Composition, Antioxidant and Enzyme Inhibitory Activities of Onosma bourgaei and Onosma trachytricha and in Silico Molecular Docking Analysis of Dominant Compounds

İstifli

2021

Molecules

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“…Thus, enzyme inhibitors from natural sources are needed to replace these synthetic ones. In recent investigations, several researchers reported enzyme inhibition properties of several Boraginaceae members such as, Alkanna sfikasiana [ 49 ], Cynoglossum creticum [ 24 ], Cynoglottis barrelieri [ 25 ], Echium confusum [ 50 ], Onosma aucheriana , Onosma sieheana , Onosma frutescens , Onosma stenoloba , and Onosma sericea [ 51 , 52 ] Symphytum anatolicum [ 25 , 26 ]. In the present study, the enzyme inhibitory effects of M. maritima extracts were investigated using different enzymes.…”

Section: Discussionmentioning

confidence: 99%

“…Among the three M. martima tissue extracts studied, calli extracts exhibited ideal AChE (IC50: 0.74 mg/mL) inhibition, seedlings extracts exhibited ideal BChE (IC50: 0.74 mg/mL) inhibition and tyrosinase (IC50: 0.74 mg/mL) inhibition, while all three extracts exhibited similar amylase (IC50: 1.40–1.47 mg/mL) inhibition activity ( Table 9 ). The IC50 values attained from tyrosinase and amylase inhibition assays were lower compared to several other Boraginaceae members [ 51 , 52 ]. We observed different results for each enzyme inhibition ability.…”

Section: Discussionmentioning

confidence: 99%

Screening of Bioactive Metabolites and Biological Activities of Calli, Shoots, and Seedlings of Mertensia maritima (L.) Gray

Song

Sivanesan

et al. 2020

Plants

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Mertensia maritima (L.) Gray is threatened with extinction owing to climate change, poor seed germination, and ocean warming. In vitro explant-culture is used for ex situ preservation and plantlet massive production. In vitro cell and organ cultures serve as an alternative plant material source to investigate the biological activities and phytochemical profiles of rare plants. We aimed to develop an efficient callus and shoot production protocol and investigate bioactive metabolites, antioxidants, and enzyme inhibitory potential of M. maritima calli, shoots, and in vivo seedlings. The effects of combinations of different plant growth regulators, 6-BA (N6-benzyladenine), 6-KN (Kinetin), TDZ (Thidiazuron), and NAA (1-Naphthylacetic acid), in MS (Murashige and Skoog) nutrient medium were studied. The highest callus proliferation was obtained after 5-week cultivation over a 16-h photoperiod on growth medium MS enriched with 4 µM each of 6-BA and NAA. The medium with 2 µM 6-BA and 4 µM 6-KN had the best shoot induction rate (91.1%) with a mean of 13.4 shoots. The combination of two cytokinins (6-BA and 6-KN) was found to be effective in M. maritima shoot regeneration. The rooting frequency was 100% in ½ MS with Indole-3-butyric acid (IBA 2 µM). The number of detected compounds and chemical composition in the M. maritima shoots and seedlings extracts were similar. The total amount of phenolics in the shoots was 216.4% and 369.5% higher than in seedlings and calli, respectively. The total amount of flavonoids in the shoots was 241.1% and 429.3% higher than in seedlings and calli, respectively. The best antioxidant activity was obtained in the shoots, followed by seedlings and calli. However, the order was seedlings > calli > shoots regarding metal chelating ability. The strongest acetylcholinesterase inhibition properties were obtained in the calli, followed by seedlings and shoots. However, the tested samples can be ranked as seedlings > shoots > calli in butylcholinestrase inhibition assay. This study is the first report on the enzyme inhibitory effects of M. maritima extracts, providing valuable contributions to the scientific community.

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Hieracium waldsteinii (Asteraceae) and Onosma stellulata (Boraginaceae) as a Source of Antioxidant and Antimicrobial Agents

Kostić

Mačukanović-Jocić

Milinčić

et al. 2022

Chemistry & Biodiversity

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The current study aimed to phytochemically characterize (including a detailed phenolic profile) two endemic Balkan's species (Hieracium waldsteinii and Onosma stellulata) and determine their possible application as a source of natural antioxidant and antimicrobial agents. The main phenolic compound in both species (in all examined parts) was chlorogenic acid. Eriodictyol, genistein and naringenin were quantified only in H. waldsteinii while isorhamnetin‐3‐O‐rutinoside and sinapic acid were characteristic for O. stellulata. The highest antioxidant activity (98 mg AAE/g dry weight for TAC assay) was ascribed to the flower extract of H. waldsteinii while the lowest results (∼4.3 mg AAE/g dry weight for FRP assay) were exhibited by the extracts obtained from the plant's stem. Antimicrobial assays showed moderate antibacterial, i. e., moderate/strong activity against several tested fungi (in particular Trichoderma viride). Correlation analysis revealed strong positive connection between phenolic compounds and reducing power of extracts as well as between total phenolic and flavonoid content and the obtained minimal inhibitory concentration recorded in antibacterial assays.

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Two endemic Onosma species (O. sieheana and O. stenoloba): A comparative study including docking data on biological activity and phenolic composition

Cited by 18 publications

References 59 publications

Chemical Composition, Antioxidant and Enzyme Inhibitory Activities of Onosma bourgaei and Onosma trachytricha and in Silico Molecular Docking Analysis of Dominant Compounds

Chemical Composition, Antioxidant and Enzyme Inhibitory Activities of Onosma bourgaei and Onosma trachytricha and in Silico Molecular Docking Analysis of Dominant Compounds

Screening of Bioactive Metabolites and Biological Activities of Calli, Shoots, and Seedlings of Mertensia maritima (L.) Gray

Hieracium waldsteinii (Asteraceae) and Onosma stellulata (Boraginaceae) as a Source of Antioxidant and Antimicrobial Agents

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