In this work we introduce the LAMBDA 4.0 toolbox, which provides an enhanced implementation for integer estimation, validation, and success rate evaluation. This free and open-source toolbox is a major update to LAMBDA 3.0 (2012), while it also integrates the functionalities from Ps-LAMBDA 1.0 (2013), thus respectively merging both estimation and evaluation capabilities. The new implementation provides redefined algorithms, such as an improved integer search strategy with a one-order reduction in the computational time, along with additional estimators: Vectorial Integer Bootstrapping (VIB), Integer Aperture Bootstrapping (IAB) and Best Integer Equivariant (BIE). This toolbox aims to become a valuable resource for researchers and/or practitioners dealing with mixed-integer models in high dimensions, e.g., terrestrial-based carrier-phase systems, multi-constellation Global Navigation Satellite Systems (GNSS), or other interferometric applications.