Two independent promoters as well as 5′untranslated regions regulate Dd<i>ras</i>expression in<i>Dictyostellium</i>

Louvion, Jean-François; Scholder, Jean-Charles; Pinaud, Stèphane; Reymond, Christophe

doi:10.1093/nar/19.22.6133

Cited by 12 publications

(3 citation statements)

References 18 publications

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“…3), but further expression was not mRNA accumulated under fast-shake conditions in the absence of added cAMP in lagC-null cells, rasD produces two transcripts (1.0 and 1.2 kb} from independent promoters (Louvion et al 1991;Esch et al 1992). The rasD 1.2-kb transcript, which is induced in fast-shake culture in wild-type cells in response to high levels of cAMP (Esch and Firtel 1991}, accumulated in lagC-null cells but to a lower level than was observed in wild-type cells (Fig.…”

Section: Developmental Gene Expression In Lagc-null Cellsmentioning

confidence: 99%

LagC is required for cell-cell interactions that are essential for cell-type differentiation in Dictyostelium.

Dynes¹,

Clark²,

Shaulsky³

et al. 1994

Genes Dev.

143

136

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Strain AK127 is a developmental mutant of Dictyostelium discoideum that was isolated by restriction enzyme-mediated integration (REMI). Mutant cells aggregate normally but are unable to proceed past the loose aggregate stage. The cloned gene, lagC (loose aggregate C), encodes a novel protein of 98 kD that contains an amino-terminal signal sequence and a putative carboxy-terminal transmembrane domain. The mutant strain AK127 shows no detectable lagC transcript upon Northern analysis, indicating that the observed phenotype is that of a null allele. Expression of the lagC cDNA in AKI27 cells complements the arrest at the loose aggregate stage, indicating that the mutant phenotype results from disruption of the lagC gene. In wild-type cells, lagC mRNA is induced at the loose aggregate stage and is expressed through the remainder of development, lagC-null cells aggregate but then disaggregate and reaggregate to form small granular mounds. Mature spores are produced at an extremely low efficiency (<0.1% of wild type), appearing only after -72 hr, whereas wild-type strains produce mature spores by 26 hr. lagC-null cells accumulate reduced levels of transcripts for the prestalk-enriched genes rasD and CP2 and do not express the DIF-induced prestalk-specific gene ecmA or the cAMP-induced prespore-specific gene SP60 to significant levels. In chimeric organisms resulting from the coaggregation of lagC-null and wild-type cells, cell-type-specific gene expression is rescued in the lagC-null cells; however, lagC-prespore cells are localized to the posterior of the prespore region and do not form mature spores, suggesting that LagC protein has both no cell-autonomous and cell-autonomous functions. Overexpression of lagC from an actin promoter in both wild-type and lagC-cells causes a delay at the tight aggregate stage, the first stage requiring LagC activity. These results suggest that the LagC protein functions as a nondiffusible cell-cell signaling molecule that is required for multicellular development.

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Section: Developmental Gene Expression In Lagc-null Cellsmentioning

confidence: 99%

LagC is required for cell-cell interactions that are essential for cell-type differentiation in Dictyostelium.

Dynes¹,

Clark²,

Shaulsky³

et al. 1994

Genes Dev.

143

136

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“…The literature search has resulted in 5 known TREs as follows. We collected Psp-3B variant Harwood [38], ras S-promoter [39], Gene 7E psp-AT type [40], ecmB Repressor [41], and MMF Elements [42]. We found these 5 known TREs in simulation results.…”

Section: Simulation Resultsmentioning

confidence: 99%

A New Approach to Predicting Regulatory Elements in Dictyostelium Gene Expression

Kim¹,

Seo²,

Yasunaga³

et al. 2006

2006 International Conference on Hybrid Information Technology

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In current bioinformatics, the prediction of transcriptional regulatory elements (TREs) is one of the major challenges. It is a key step toward deciphering entire genetic regulatory networks. The goal of this study is to predict TREs associated with four classes of developmentally regulated genes in Dictyostelium discoideum (Dd). Applying a pattern recognition method to 5' untranscribed region of Dd sequences, the evaluation score (E-score) for each candidate TRE is calculated. Based on these E-scores and the proposed selection criteria, putative class-specific TREs are predicted in each developmentally regulated class of Dd.

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“…Published and Genbank sequences differed for disc A; the published seqtlence was used. The Harwood element at 173 in rasD is present inLouvion et al's (1991) sequence, but not that of Eseh et ah(1992). Where rnditiple st ar t-o f4rans cription sites were indicated in published material, numbering is from the farthest upstream site.…”

mentioning

confidence: 76%

Evolution of Promoter Sequences: Elements of a Canonical Promoter for Prespore Genes of Dictyostelium

1996

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An attempt is made to define a minimal prespore promoter which contains all elements essential for correct regulation of expression of a prespore gene. The prespore genes of Dictyostelium are coregulated during development. Most begin transcription at the same early stage, and activity of all is restricted to prespore tissue during the later slug stage. Sequences 5' to the coding sequences of eight prespore genes were searched for all elements proposed to control transcription and for new elements. The meaningfulness of occurrences of elements and pairs of elements in prespore promoters was evaluated by comparison with frequencies of occurrences in promoters of other, nonprespore genes. These comparisons resulted in definition of a canonical prespore promoter, a stretch of about 200 nucleotides conmining at least one of each of three elements. Certain limitations were found on the spacing of elements. Orientation of elements with respect to each other appeared unrestricted. All elements often occurred in multiple copies. This structure suggests that individual copies of each element are not conserved during evolution, but instead continually appear and disappear.

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Two independent promoters as well as 5′untranslated regions regulate Ddrasexpression inDictyostellium

Cited by 12 publications

References 18 publications

LagC is required for cell-cell interactions that are essential for cell-type differentiation in Dictyostelium.

LagC is required for cell-cell interactions that are essential for cell-type differentiation in Dictyostelium.

A New Approach to Predicting Regulatory Elements in Dictyostelium Gene Expression

Evolution of Promoter Sequences: Elements of a Canonical Promoter for Prespore Genes of Dictyostelium

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