Two kinds of electrochemical immunoassays for the tumor necrosis factor α in human serum using screen-printed graphite electrodes modified with poly(anthranilic acid)

Mazloum-Ardakani, Mohammad; Hosseinzadeh, Laleh; Taleat, Zahra

doi:10.1007/s00604-014-1186-9

Cited by 31 publications

(17 citation statements)

References 33 publications

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“…Nowadays, polyaniline (PANI) as an effectively conductive electroactive polymer has increasing application in the field of electrochemical DNA biosensors [40,41]. Especially, the excellent properties of PANI nanofibers, such as high surface area, mechanical flexibility, tunable conductivity, chemical specificity, and easy processing make it the potential material for ultrasensitive and tracelevel nanosensors [42][43][44][45].…”

Section: Introductionmentioning

confidence: 99%

Employing Label‐free Electrochemical Biosensor Based on 3D‐Reduced Graphene Oxide and Polyaniline Nanofibers for Ultrasensitive Detection of Breast Cancer BRCA1 Biomarker

Xia

Chen

et al. 2020

Electroanalysis

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A label‐free DNA biosensor based on three‐dimensional reduced graphene oxide (3D‐rGO) and polyaniline (PANI) nanofibers modified glassy carbon electrode (GCE) was successfully developed for supersensitive detection of breast cancer BRCA1. The results demonstrated that 3D‐rGO and PANI nanofibers had synergic effects for reducing the charge transfer resistance (Rct), meaning a huge enhancement in electrochemical activity of 3D‐rGO‐PANI/GCE. Probe DNA could be immobilized on 3D‐rGO‐PANI/GCE for special and sensitive recognition of target DNA (1.0×10−15–1.0×10−7 M) with a theoretical LOD of 3.01×10−16 M (3S/m). Furthermore, this proposed nano‐biosensor could directly detect BRCA1 in real blood samples.

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Section: Introductionmentioning

confidence: 99%

Employing Label‐free Electrochemical Biosensor Based on 3D‐Reduced Graphene Oxide and Polyaniline Nanofibers for Ultrasensitive Detection of Breast Cancer BRCA1 Biomarker

Xia

Chen

et al. 2020

Electroanalysis

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“…As Table 1 shows [14][15][16][17][18][19][20][21][22][23][24][25][26][27][28][29][30], the number of sensors described for TNF-α is relatively high whereas those for IL-β1 are very scarce. Diverse electrode modifiers [15,16], signal amplifiers [16,17] and a variety of electrochemical techniques [18,19] have been employed to attain the required detection limits. Regarding the simultaneous determination of these cytokines, only three electrochemical immunoassay configurations have been reported (Table 1).…”

Section: Introductionmentioning

confidence: 99%

“…2b displays the calibration plot for TNF-α with an equation I, nA = (746 ± 16) log C, pg/mL + (245 ± 22) (r 2 = 0.999), with a range of linearity from 1 to 200 pg/mL.It is worth to mention that these ranges, covering two orders of magnitude, are adequate for the determination of the cytokines in clinical samples taking into account the expected concentrations at the level of few pg/mL[19]. The achieved limits of detection were 0.38 pg/mL (IL-1β) and 0.85 pg/mL (TNF-α) and were calculated by using the 3s b criterion, where s b was estimated as the standard deviation expressed in concentration units (n =10) of the measured currents for the blank (0 pg/mL IL-1β or TNF-α).…”

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confidence: 99%

Electrochemical immunosensor for simultaneous determination of interleukin-1 beta and tumor necrosis factor alpha in serum and saliva using dual screen printed electrodes modified with functionalized double–walled carbon nanotubes

Sánchez‐Tirado

Salvo

González‐Cortés

et al. 2017

Analytica Chimica Acta

130

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Dual screen-printed carbon electrodes modified with 4-carboxyphenyl-functionalized double-walled carbon nanotubes (HOOC-Phe-DWCNTs/SPCEs) have been used as scaffolds for the preparation of electrochemical immunosensors for the simultaneous determination of the cytokines Interleukin-1β (IL-1β) and factor necrosis tumor α (TNF-α). IL-1β. Capture antibodies were immobilized onto HOOC-Phe-DWCNTs/SPCEs in an oriented form making using the commercial polymeric coating Mix&Go™. Sandwich type immunoassays with amperometric signal amplification through the use of poly-HRP-streptavidin conjugates and HO as HRP substrate and hydroquinone as redox mediator were implemented. Upon optimization of the experimental variables affecting the immunosensor performance, the dual immunosensor allows ranges of linearity extending between 0.5 and 100 pg/mL and from 1 to 200 pg/mL for IL-1β and TNF-α, respectively, these ranges being adequate for the determination of the cytokines in clinical samples. The achieved limits of detection were 0.38 pg/mL (IL-1β) and 0.85 pg/mL (TNF-α). In addition, the dual immunosensor exhibits excellent reproducibility of the measurements, storage stability of the anti-IL-Phe-DWCNTs/SPCE and anti-TNF-Phe-DWCNTs/SPCE conjugates, and selectivity as well as negligible cross-talking. The dual immunosensor was applied to the simultaneous determination of IL-1β and TNF-α in human serum spiked at clinically relevant concentration levels and in real saliva samples.

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“…[3][4][5][6][7] Many different electrochemical methods were used in order to fabrication of immunosensor and detection of biomarkers. [3][4][5][6][7] Many different electrochemical methods were used in order to fabrication of immunosensor and detection of biomarkers.…”

Section: Introductionmentioning

confidence: 99%

Highly-sensitive label-free immunosensor for tumor necrosis factor α based on Ag@Pt core–shell nanoparticles supported on MWCNTs as an efficient electrocatalyst nanocomposite

Mazloum-Ardakani

Hosseinzadeh

2015

RSC Adv.

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We have developed a novel and label-free electrochemical immunosensor for detection of tumor necrosis factor-alpha (TNF-a) based on bimetallic silver@platinum (Ag@Pt) core-shell nanoparticles supported on MWCNTs and chitosan (Ag@Pt-CNTs-CS) as a desirable sensor platform. The redox reaction of catechol on the graphite screen-printed electrodes modified with Ag@Pt-CNTs-CS applied as a probe for studying the immobilization and determination processes in immunosensor, due to enhanced electrocatalytic activity of Ag@Pt-CNTs for catechol. The results showed that this nanocomposite exhibited attractive electrocatalytic activity and also yielded a large capacity for antibody loading, which improved the amount of immobilized antibody. A decrease in differential pulse voltammetry (DPV) responses was observed with increasing concentrations of TNF-a in standard and real samples due to its obstruction to the electrocatalytic oxidation of catechol by Ag@Pt-CNTs after binding to the surface of the electrode through interaction with the antibody. The detection limit for TNF-a was found to be 1.6 pg mL À1 and the detection concentrations were in the range between 6.0 and 60 pg mL À1 . The proposed electrochemical immunosensor showed high sensitivity, specificity, good reproducibility and stability as well as acceptable accuracy for TNF-a detection in human serum samples and this strategy might also be applied to detect other antigens.

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Two kinds of electrochemical immunoassays for the tumor necrosis factor α in human serum using screen-printed graphite electrodes modified with poly(anthranilic acid)

Cited by 31 publications

References 33 publications

Employing Label‐free Electrochemical Biosensor Based on 3D‐Reduced Graphene Oxide and Polyaniline Nanofibers for Ultrasensitive Detection of Breast Cancer BRCA1 Biomarker

Employing Label‐free Electrochemical Biosensor Based on 3D‐Reduced Graphene Oxide and Polyaniline Nanofibers for Ultrasensitive Detection of Breast Cancer BRCA1 Biomarker

Electrochemical immunosensor for simultaneous determination of interleukin-1 beta and tumor necrosis factor alpha in serum and saliva using dual screen printed electrodes modified with functionalized double–walled carbon nanotubes

Highly-sensitive label-free immunosensor for tumor necrosis factor α based on Ag@Pt core–shell nanoparticles supported on MWCNTs as an efficient electrocatalyst nanocomposite

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