Two-Layer Tissue Engineered Urethra Using Oral Epithelial and Muscle Derived Cells

Mikami, Hiroshi; Kuwahara, Go; Nakamura, Nobuyuki; Yamato, Masayuki; Tanaka, Masatoshi; Kodama, Shohta

doi:10.1016/j.juro.2011.12.059

Cited by 34 publications

(31 citation statements)

References 30 publications

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“…Previously, Mikami et al . 38 fabricated two-layer tissue-engineered urethra grafts by combining autologous oral epithelial cell sheets with collagen mesh matrices seeded with muscle cells that were grafted to cover a 2-cm urethral defect in a canine model and promoted the regeneration of a functional urethra. However, the 2-layer urethra without a fibrous layer is a loose construction that may lack adequate mechanical strength and revascularization for the submucosal layer of urethral vessels could not be regenerated.…”

Section: Discussionmentioning

confidence: 99%

Fabrication of Tissue-Engineered Bionic Urethra Using Cell Sheet Technology and Labeling By Ultrasmall Superparamagnetic Iron Oxide for Full-Thickness Urethral Reconstruction

Zhou¹,

Yang²,

Zou³

et al. 2017

Theranostics

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Urethral strictures remain a reconstructive challenge, due to less than satisfactory outcomes and high incidence of stricture recurrence. An “ideal” urethral reconstruction should establish similar architecture and function as the original urethral wall. We fabricated a novel tissue-engineered bionic urethras using cell sheet technology and report their viability in a canine model. Small amounts of oral and adipose tissues were harvested, and adipose-derived stem cells, oral mucosal epithelial cells, and oral mucosal fibroblasts were isolated and used to prepare cell sheets. The cell sheets were hierarchically tubularized to form 3-layer tissue-engineered urethras and labeled by ultrasmall super-paramagnetic iron oxide (USPIO). The constructed tissue-engineered urethras were transplanted subcutaneously for 3 weeks to promote the revascularization and biomechanical strength of the implant. Then, 2 cm length of the tubularized penile urethra was replaced by tissue-engineered bionic urethra. At 3 months of urethral replacement, USPIO-labeled tissue-engineered bionic urethra can be effectively detected by MRI at the transplant site. Histologically, the retrieved bionic urethras still displayed 3 layers, including an epithelial layer, a fibrous layer, and a myoblast layer. Three weeks after subcutaneous transplantation, immunofluorescence analysis showed the density of blood vessels in bionic urethra was significantly increased following the initial establishment of the constructs and was further up-regulated at 3 months after urethral replacement and was close to normal level in urethral tissue. Our study is the first to experimentally demonstrate 3-layer tissue-engineered urethras can be established using cell sheet technology and can promote the regeneration of structural and functional urethras similar to normal urethra.

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Section: Discussionmentioning

confidence: 99%

Fabrication of Tissue-Engineered Bionic Urethra Using Cell Sheet Technology and Labeling By Ultrasmall Superparamagnetic Iron Oxide for Full-Thickness Urethral Reconstruction

Zhou¹,

Yang²,

Zou³

et al. 2017

Theranostics

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“…It is showing promising results in preclinical and clinical trials for future implication as an alternative cell source for urethral tissue engineering 35,106,107. In a recent clinical study involving 12 patients, stricture recurrence in the anterior urethra occurred in two patients only 108.…”

Section: Biofabrication Of the Urinary Tractmentioning

confidence: 99%

Biofabrication and biomaterials for urinary tract reconstruction

Elsawy

Mel

2017

RRU

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Reconstructive urologists are constantly facing diverse and complex pathologies that require structural and functional restoration of urinary organs. There is always a demand for a biocompatible material to repair or substitute the urinary tract instead of using patient’s autologous tissues with its associated morbidity. Biomimetic approaches are tissue-engineering tactics aiming to tailor the material physical and biological properties to behave physiologically similar to the urinary system. This review highlights the different strategies to mimic urinary tissues including modifications in structure, surface chemistry, and cellular response of a range of biological and synthetic materials. The article also outlines the measures to minimize infectious complications, which might lead to graft failure. Relevant experimental and preclinical studies are discussed, as well as promising biomimetic approaches such as three-dimensional bioprinting.

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“…However, the process of graft take in an injured urethra may substantially differ from that in a normal urethral bed. What is more, only four (Mikami et al , ; Orabi et al , ; Xie et al , , ) of 28 preclinical studies used large animals (dogs), and only two of them used male dogs (Mikami et al , ; Orabi et al , ). While we are aware that, anatomically, the female and male urethras are different, and that spongiofibrosis, being the major cause of strictures in males, never occurs in females, we still decided to include the studies conducted on female animals in our review.…”

Section: Current Challenges and Future Directionsmentioning

confidence: 99%

“…Mikami et al () formed tubularized tissue‐engineered urethras comprised of collagen mesh matrices and autologous cells of two types – keratinocytes and buccal mucosa smooth muscle cells. These grafts were used to repair urethral defects in the experimental group (10 dogs) and were not applied to defects in the control group (10 dogs).…”

Section: Introduction To Tissue Engineeringmentioning

confidence: 99%

The potential role of tissue-engineered urethral substitution: clinical and preclinical studies

Atala

Danilevskiy

Lyundup

et al. 2015

J Tissue Eng Regen Med

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Urethral strictures and anomalies remain among the difficult problems in urology, with urethroplasty procedures being the most effective treatment options. The two major types of urethroplasty are anastomotic urethroplasty and widening the urethral lumen using flaps or grafts (i.e. substitution urethroplasty). However, no ideal material for the latter has been found so far. Designing and selecting such a material is a necessary and challenging endeavour, driving the need for further bioengineered urethral tissue research. This article reviews currently available studies on the potentialities of tissue engineering in urethral reconstruction, in particular those describing the use of both acellular and recellularized tissue-engineered constructs in animal and human models. Possible future developments in this field are also discussed. Copyright © 2015 John Wiley & Sons, Ltd.

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Two-Layer Tissue Engineered Urethra Using Oral Epithelial and Muscle Derived Cells

Abstract: Two-layer tissue engineered urethras were engineered using cells harvested by minimally invasive oral punch biopsy. Results suggest that this technique can encourage regeneration of a functional urethra.

Cited by 34 publications

References 30 publications

Fabrication of Tissue-Engineered Bionic Urethra Using Cell Sheet Technology and Labeling By Ultrasmall Superparamagnetic Iron Oxide for Full-Thickness Urethral Reconstruction

Fabrication of Tissue-Engineered Bionic Urethra Using Cell Sheet Technology and Labeling By Ultrasmall Superparamagnetic Iron Oxide for Full-Thickness Urethral Reconstruction

Biofabrication and biomaterials for urinary tract reconstruction

The potential role of tissue-engineered urethral substitution: clinical and preclinical studies

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