Two lineages of<i>Trifolium alpinum</i>(Fabaceae) in the Pyrenees: evidence from random amplified polymorphic DNA (RAPD) markers

Lauga, Béatrice; Malaval, Sandra; Largier, Gérard; Regnault-Rogert, Catherine

doi:10.1080/12538078.2009.10516162

Cited by 10 publications

(6 citation statements)

References 40 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In our view, it can be proposed that this hexaploid race represents an old Pyrenean refugium, where it has persisted in isolation since the last glacial maximum. A similar biogeographic pattern of differentiation between Pyrenean populations has been revealed in several other plant (Kropf et al 2003, Lauga et al 2009, Charrier et al 2014) and animal species (Milá et al 2010, Bidegaray-Batista et al 2016.…”

Section: Comparison With Earlier Studies Based On Chromosome Countingsupporting

confidence: 70%

DNA ploidy in Salix retusa agg. only partly in line with its morphology and taxonomy

Kosiński

Śliwińska

Hilpold

et al. 2019

Nordic Journal of Botany

View full text Add to dashboard Cite

Salix retusa agg. encompasses small, prostrate willows occurring in the subalpine and alpine zones of European mountains. Karyological relationships among taxa in this group remain unclear. In this study, DNA ploidy was estimated using flow cytometry of silica‐dried specimens, covering the entire distribution area of the species. As a result, the presence of two main lineages was revealed: the exclusively diploid S. serpillifolia and the predominantly octoploid S. retusa s.l. Only at the western and eastern limits of the distributional range of the latter species different levels of DNA ploidy occurred: hexaploidy in the western Pyrenees, and up to decaploidy in the southern Carpathians. These results were compared with leaf morphology analyses presented in the work of Kosiński et al. (2017). In the western Pyrenean populations, the lower ploidy corresponded to smaller leaf sizes as compared to other populations of S. retusa s.l. The elevated ploidy in the southern Carpathian populations did not translate into bigger dimensions of plants in this area. However, the populations from the northern part of the Carpathian arc, distinguished by larger leaves and sometimes considered as a distinct taxon (S. kitaibeliana), consisted mainly of octoploid individuals. Thus, DNA ploidy estimations did not confirm the separate taxonomic status of S. kitaibeliana.

show abstract

Section: Comparison With Earlier Studies Based On Chromosome Countingsupporting

confidence: 70%

DNA ploidy in Salix retusa agg. only partly in line with its morphology and taxonomy

Kosiński

Śliwińska

Hilpold

et al. 2019

Nordic Journal of Botany

View full text Add to dashboard Cite

show abstract

“…However, the most often observed number of different lineages in animals and plants found here is two (e.g. Kropf et al 2002Kropf et al , 2003Schmitt et al 2006;Lauga et al 2009), translating into only two glacial refugia (and differentiation centres) in close proximity to the Pyrenees and not four as in the much bigger Alps. Higher numbers of different lineages in the Pyrenees Fig.…”

Section: Different Genetic Lineages Within High Mountain Systemsmentioning

confidence: 96%

Molecular Biogeography of the High Mountain Systems of Europe: An Overview

Schmitt

2017

Advances in Global Change Research

View full text Add to dashboard Cite

The biogeography of alpine and arctic-alpine species is complex, much more complex than thought until relatively recently. Alpine species survived glacial periods mostly within refugia in close proximity to the mountains where they are found today. One mountain range can be colonised from several glacial refugia, while one refugium can be the source of colonisation of more than one mountain range. The zonal distributions in the glacial cold steppes are only of importance for arctic-alpine species. Their arctic ranges normally derive from there, while the southern mountains were colonised from there or from near-mountain refugia as in the cases of the alpine species.

show abstract

“…Our work aims to compare the use of different reference species for estimating nitrogen f ixation in T. alpinum-dominant communities in a 3-year experiment. T. alpinum is a leguminous species widespread on different southern European mountain chains (Alps, Pyrenees, North Apennine, and Central Massif) (Aeschimann et al 2004 ;Lauga et al 2009). In the alpine belt of the southwestern Alps (Italy and France), T. alpinum frequently occurs as a dominant or co-dominant species, accounting for 30--40% or more of total above-ground phytomass (Bassignana and Bomard 2001;Bornard et al 2007;Lombardi et al 2007;Falchero et al 2010 ).…”

Section: Introductionmentioning

confidence: 99%

Nitrogen fixation assessment in a legume-dominant alpine community: comparison of different reference species using the 15N isotope dilution technique

et al. 2014

View full text Add to dashboard Cite

In arctic and alpine environments the highest biologically fixed nitrogen inputs are measured in the presence of leguminous plants, with a maximum annual nitrogen input recorded of about 10 kg ha-1 year-1. Among the high-elevation legumes, Trifolium alpinum spreads over many southern European mountain grasslands as a dominant or eo-dominant species, accounting for 30-40% or more of total above-ground phytomass. We estimated the amount of symbiotic nitrogen fixation in T. alpinum-dominant communities using the 15 N isotope dilution technique and comparing the calculations derived from different non-fixing reference species. The estimated percentage of nitrogen derived from the atmosphere varied significantly depending on the reference species used and they were comparable to the ones reported in literature. No differences were detected among years. In the community studied, the use of one reference species instead of another can yield differences of more than 10 kg ha-1 year-1 , which may represent a considerable difference in the estimation of symbiotic nitrogen fixation in alpine ecosystems. Taking a very precautionary approach and using the grass Nardus stricta as the reference species, we estimated 21.9 kg ha-1 year-1 of nitrogen fixation in T. alpinumdominant communities. This quantity of nitrogen is the highest ever estimated in a natural alpine grassland and it is related to the high annual above-ground phytomass of T. alpinum.

show abstract

Two lineages ofTrifolium alpinum(Fabaceae) in the Pyrenees: evidence from random amplified polymorphic DNA (RAPD) markers

Cited by 10 publications

References 40 publications

DNA ploidy in Salix retusa agg. only partly in line with its morphology and taxonomy

DNA ploidy in Salix retusa agg. only partly in line with its morphology and taxonomy

Molecular Biogeography of the High Mountain Systems of Europe: An Overview

Nitrogen fixation assessment in a legume-dominant alpine community: comparison of different reference species using the 15N isotope dilution technique

Contact Info

Product

Resources

About