Two micro-scale protocols for the isolation of DNA from polysaccharide-rich plant tissue

Shepherd, Lara D.; McLay, Todd G. B.

doi:10.1007/s10265-010-0379-5

Cited by 98 publications

(70 citation statements)

References 15 publications

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“…Sampled leaves were dried with silica gel immediately after collection. DNA solutions extracted from the dried specimens using STE/CTAB method following Shepherd and McLay (2011). Eight primers were available for V. p. lilacina (Table 2).…”

Section: Issr-pcr Analysismentioning

confidence: 99%

Island biogeography as a test of reproductive interference

Takakura

Fujii

2015

Population Ecology

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Theoretical studies have predicted that reproductive interference must exclude either of the interacting species, but no testing of this prediction has ever been reported for natural populations. This study surveyed the distribution patterns of herbaceous Veronica plants, including one native and three alien species, to test whether the invasion of the alien species exerting reproductive interference excluded the native species. Results showed that the native species was repeatedly excluded from islands where an alien species invaded, exerting reproductive interference, and that other alien species had no significant effect on the native population survival. This survey also demonstrated that the native species altered its habitat from the ground to stone walls on the mainland where the alien species had been predominant. In the mainland populations, the fruit morphology differed from that of the islands, and the morphology in the mainland population seemed suitable for seed dispersion by ants at a stone wall habitat. We also surveyed the genetic differentiation among populations, the results of which did not support the native species genetically differentiated between mainland and island populations before the alien species invasion.These results strongly suggest that the reproductive interference excluded the recipient species at the population level and facilitated the habitat change. Additionally, results indicated that a series of field surveys of islands close to the mainland can be a powerful tool to test the ecological importance of reproductive interference.

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Section: Issr-pcr Analysismentioning

confidence: 99%

Island biogeography as a test of reproductive interference

Takakura

Fujii

2015

Population Ecology

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“…Genomic DNA was extracted from fresh young leaf tissue from 15 seedlings using a modifi ed cetyltrimethylammonium bromide (CTAB) method ( Shepherd and McLay, 2011 ). To generate suffi cient template for the requirements of Illumina MiSeq library preparation, extracted DNA was pooled and amplifi ed using a REPLI-g kit (QIAGEN, Hilden, Germany) following the manufacturer's protocol.…”

Section: Methods and Resultsmentioning

confidence: 99%

Morphological and amplified fragment length polymorphism (AFLP) data show that New Zealand endemic Myosotis petiolata (Boraginaceae) comprises three rare and threatened species

Meudt¹,

Prebble²,

Stanley³

et al. 2013

Aust. Systematic Bot.

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Species delimitation is of critical importance in systematics and biological and conservation research. The general-lineage species concept, which defines species as separately evolving metapopulation lineages, considers multiple lines of evidence to identify lineages and delimit species boundaries. Here, we apply the general-lineage concept to the New Zealand endemic Myosotis petiolata Hook.f. (Boraginaceae) species complex, to test its usefulness in the New Zealand Myosotis L. species radiation. We aimed to determine whether the complex contains separately evolving lineages to assess the criteria of monophyly, distinct genotypic clusters and fixed morphological differences by using amplified fragment length polymorphism (AFLP) and morphological data. The use of multiple criteria to identify separately evolving lineages within the M. petiolata complex was effective, but the different criteria were satisfied to varying degrees. Species rank is recommended for each of the currently recognised varieties as Myosotis pottsiana (L.B.Moore) Meudt, Prebble, R.J.Stanley & Thorsen (comb. & stat. nov.), M. pansa (L.B.Moore) Meudt, Prebble, R.J.Stanley & Thorsen (comb. & stat. nov.) and M. petiolata Hook.f. (North Island individuals only). A new allopatric subspecies, M. pansa subsp. praeceps Meudt, Prebble, R.J.Stanley & Thorsen (subsp. nov.), is also described. The distinguishing morphological characters and conservation status of each species are discussed. In addition, the low genetic diversity revealed in our population genetic analyses, coupled with few, fluctuating, disjunct populations, underscores the conservation priority of these three rare endemic New Zealand species.

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“…DNA was extracted from ~20 mg dry tissue of both Chamaescilla corymbosa (MELU M113170a) and Hodgsoniola junciformis (Mueller 1860: 96) Mueller (1861PERTH 7488793) using a modified CTAB protocol (Shepherd & McLay 2011). The atpB, rbcL, ndhF, and matK regions were amplified using the primers of Chen et al (2013; Table 2).…”

Section: Methodsmentioning

confidence: 99%

A new family placement for Australian blue squill, Chamaescilla: Xanthorrhoeaceae (Hemerocallidoideae), not Asparagaceae

McLay

Bayly

2016

Phytotaxa

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Chamaescilla is an endemic Australian genus, currently placed in the Asparagaceae, alongside other Australian endemic taxa in the tribe Lomandroideae. A recent molecular phylogeny indicated a relationship with another partly Australian family, the Xanthorrhoeaceae, but was not commented on by the authors. Here we added DNA sequence data for a single Chamaescilla specimen to an alignment representing all families in the Asparagales and performed parsimony and Bayesian phylogenetic analyses. Chamaescilla was strongly resolved as belonging to Xanthorrhoeaceae, subfamily Hemerocallidoideae, alongside two non-Australian members, Simethis and Hemerocallis in the hemerocallid clade. This position is corroborated by morphological characters, including pollen grain shape. We also produced an age-calibrated phylogeny and infer that the geographic distribution of the clade is the result of long distance dispersal between the Eocene and Miocene.

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Two micro-scale protocols for the isolation of DNA from polysaccharide-rich plant tissue

Cited by 98 publications

References 15 publications

Island biogeography as a test of reproductive interference

Island biogeography as a test of reproductive interference

Morphological and amplified fragment length polymorphism (AFLP) data show that New Zealand endemic Myosotis petiolata (Boraginaceae) comprises three rare and threatened species

A new family placement for Australian blue squill, Chamaescilla: Xanthorrhoeaceae (Hemerocallidoideae), not Asparagaceae

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