Two New 3,4;9,10-<i>seco</i>-Cycloartane Type Triterpenoids from<i>Illicium difengpi</i>and Their Anti-Inflammatory Activities

Li, Chuntong; Xi, Feng‐Min; Mi, Jun-Ling; Wu, Zhijun; Chen, Wansheng

doi:10.1155/2013/942541

Cited by 5 publications

(4 citation statements)

References 33 publications

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“…Previous studies on I. difengpi have mostly addressed the extraction and pharmacological activity of the chemical components of its bark. Dozens of chemical components, such as phenylpropanoids [8], aromatic glycosides [9], triterpenes [10], sesquiterpenes [11] and volatile oils [12], have been isolated from I. difengpi thus far. However, I. difengpi is an endangered species.…”

Section: Introductionmentioning

confidence: 99%

Physiological and Transcriptomic Responses of Illicium difengpi to Drought Stress

Liu

Liang

et al. 2022

Sustainability

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Illicium difengpi Kib and Kim, an endangered plant unique to karst areas in China, has evolved an extremely high tolerance to arid environments. To elucidate the molecular mechanisms of the response to drought stress in I. difengpi, physiological index determination and transcriptome sequencing experiments were conducted in biennial seedlings grown under different soil moisture conditions (70~80%, 40~50% and 10~20%). With increasing drought stress, the leaf chlorophyll content decreased, while the proline (Pro), soluble sugar (SS) and malondialdehyde (MDA) contents increased; superoxide dismutase (SOD) and peroxidase (POD) activities also increased. Transcriptome sequencing and pairwise comparisons of the treatments revealed 2489, 4451 and 753 differentially expressed genes (DEGs) in CK70~80 vs. XP40~50, CK70~80 vs. XP10~20 and XP40~50 vs. XP10~20, respectively. These DEGs were divided into seven clusters according to their expression trends, and the Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment results of different clusters indicated that genes in the hormone signal transduction and osmotic regulation pathways were greatly activated under mild drought stress. When drought stress increased, the DEGs related to membrane system and protein modification and folding were all upregulated; simultaneously, chitin catabolism- and glycolysis/gluconeogenesis-related genes were continuously upregulated throughout drought stress, while the genes involved in photosynthesis were downregulated. Here, 244 transcription factors derived from 10 families were also identified. These results lay a foundation for further research on the adaptation of I. difengpi to arid environments in karst areas and the establishment of a core regulatory relationship in its drought resistance mechanism.

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Section: Introductionmentioning

confidence: 99%

Physiological and Transcriptomic Responses of Illicium difengpi to Drought Stress

Liu

Liang

et al. 2022

Sustainability

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“…Recent studies have demonstrated that squalene has anti-inflammatory activity both in vivo and in vitro, by inhibiting the nuclear factor kappa B and the production of proinflammatory cytokines (IL-1b and TNF-a). It also downregulated cyclooxygenase 2 and inducible nitric oxide synthase (Li et al 2013;Sanchez-Fidalgo et al 2015).…”

Section: Resultsmentioning

confidence: 96%

Anti-inflammatory, free radical scavenging and alpha-glucosidase inhibitory activities of Hamelia patens and its chemical constituents

Jiménez-Suárez

Nieto‐Camacho

Manuel

et al. 2016

Pharmaceutical Biology

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Context Hamelia patens Jacq. (Rubiaceae) is traditionally used to treat wounds, inflammation and diabetes. However, there is still a lack of scientific evidence to support these applications. Objective The objective of this study is to evaluate the anti-inflammatory, antioxidant and antidiabetic activities of Hamelia patens, and identify its bioactive compounds. Materials and methods Four extracts were obtained by maceration and liquid-liquid extraction: HEX, DCM-EtOAc, MeOH-EtOAc and MeOH-Aq. The anti-inflammatory effect was evaluated orally on rat paw carrageenan-induced oedema over 6 h (50, 200 and 500 mg/kg), and topically in mouse ear oedema induced by 12-tetradecanoylphorbol-13-acetate (TPA) after 4 h (0.5 and 1 mg/ear). We also evaluated myeloperoxidase levels in ear tissue, 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging ability, and in vitro α-glucosidase inhibition. The chemical compounds were separated by column chromatography and identified by spectroscopic analysis. Results We found that the oral administration of the HEX extract at 500 and 200 mg/kg significantly decreased the carrageenan-induced inflammation after 1 and 3 h, respectively. The MeOH-EtOAc extract significantly inhibited myeloperoxidase activity (83.5%), followed by the DCM-EtOAc extract (76%), β-sitosterol/stigmasterol (72.7%) and the HEX extract (55%), which significantly decreased oedema induced by TPA at both doses, giving a similar effect to indomethacin. We also found that the MeOH-EtOAc, MeOH-Aq and DCM-EtOAc extracts showed good DPPH scavenging activity (IC50 values of 18.6, 93.9 and 158.2 μg/mL, respectively). The HEX extract showed the lowest α-glucosidase inhibition (an IC50 value of 26.07 μg/mL), followed by the MeOH-EtOAc extract (an IC50 value of 30.18 μg/mL), β-sitosterol/stigmasterol (IC50 34.6 μg/mL) and compound A ((6E,10E,14E,18E)-2,6,10,14,18,23-hexamethyl-2,6,10,14,18,22-tetracosahexaene, an IC50 value of 114.6 μg/mL), which were isolated for the first time from Hamelia patens. Discussion and conclusion Hamelia patens possesses anti-inflammatory, antioxidant and α-glucosidase inhibitory activities, which support its traditional use. These effects can be attributed to the identified compounds.

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“…Other phytoconstituents in JT extracts, such as squalene from Cynodon dactylon, phytol from Erythrina variegate and Azadirachta indica , and stigmasterol derived from Pongamia pinata may also contribute to the biological effects of the JT extracts. Studies have demonstrated that squalene promotes remodelling and tissue repair response signals by inhibiting the nuclear factor-kappa B, inducible nitric oxide synthase, the production of proinflammatory cytokines, such as IL-1 β and TNF- α , and stimulation of anti-inflammatory cytokines IL-10 and IL-4 [ 46 , 47 ]. The biological activities of phytol and phytosterols can be attributed to anti-inflammatory activities through reduction of leukocyte and neutrophil migration, IL-1 β , IL-6, and TNF- α levels and oxidative stress, as well as antibacterial effects [ 48 – 50 ].…”

Section: Discussionmentioning

confidence: 99%

Antibacterial and Anti-Inflammatory Potential of Polyherbal Formulation Used in Chronic Wound Healing

Mandrika

Kumar

Zandersone

et al. 2021

Evidence-Based Complementary and Alternative Medicine

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Objective. Polyherbal formulations Jathyadi Thailam and Jatyadi Ghritam (JT) are used in Indian traditional medicine for diabetic chronic wounds, fistula, fissure, eczema, and burn management. We aimed to investigate the antibacterial and anti-inflammatory properties of crude hexane and ethanol extracts of JT formulations. Methods. Antibacterial activity of JT extracts was tested to estimate minimum inhibitory concentrations (MICs) against nine reference bacterial strains, including one methicillin-resistant Staphylococcus aureus (MRSA) and multidrug-resistant (MDR) Pseudomonas aeruginosa, and clinical strains of methicillin-susceptible S.aureus (MSSA), all involved in diabetic foot infection. The anti-inflammatory activity of plant extracts was evaluated in LPS-treated macrophage cells by measuring the mRNA levels and secretion of inflammatory mediators. Results. The antibacterial activity of JT extracts was higher against Gram (+) bacteria, with the MICs varying from 1.95 to 62.5 mg/mL. Gram (−) bacteria were only susceptible to ethanol extracts of JT. Plant extracts were found to be the most active against the reference and clinical strains of MSSA, MRSA, and biofilm-forming S. epidermidis. JT extracts efficiently inhibited in a dose-dependent manner the mRNA expression and protein secretion of proinflammatory cytokines IL-6 and IL-1β, and chemokines MCP-1 and CXCL10 in LPS-challenged macrophages. Conclusion. In the present study, we have shown that extracts of JT formulations possess potent antibacterial and anti-inflammatory properties that could be involved in chronic wound healing activity and has the potential to be used as external add-on therapy in the management of multidrug-resistant bacterial infections at the wound.

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Two New 3,4;9,10-seco-Cycloartane Type Triterpenoids fromIllicium difengpiand Their Anti-Inflammatory Activities

Cited by 5 publications

References 33 publications

Physiological and Transcriptomic Responses of Illicium difengpi to Drought Stress

Physiological and Transcriptomic Responses of Illicium difengpi to Drought Stress

Anti-inflammatory, free radical scavenging and alpha-glucosidase inhibitory activities of Hamelia patens and its chemical constituents

Antibacterial and Anti-Inflammatory Potential of Polyherbal Formulation Used in Chronic Wound Healing

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