Two new bacterial DNA primase inhibitors from the plant Polygonum cuspidatum

Hegde, Vinod R.; Pu, Haiyan; Patel, Mahesh; Black, Todd A.; Soriano, Aileen; Zhao, Wenran; Gullo, Vincent P.; Chan, Tze‐Ming

doi:10.1016/j.bmcl.2004.02.006

Cited by 50 publications

(34 citation statements)

References 10 publications

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“…The flavonoid concentration in Polygonum cuspidatum was 62.3 ± 6 mg/g, lower than Polygonum aviculare L. (112.7 ± 13 mg/g), but higher than propolis (23). The higher content of total phenolics and flavonoids in Polygonum cuspidatum have been correlated with hepatitis B virus inhibition (13), bacterial DNA primase inhibition (15), acyl-coenzyme A acyltransferase activity (16), inhibition of copper catalyzed oxidation for low-density lipoprotein (32), inhibition of platelet clotting and arachidonate metabolism, liver injury from peroxidized oil (33), having cancer-chemopreventive activities (34), and estrogenic activities (14,35). Taken together, this study indicates that Polygonum cuspidatum extract clearly has antioxidant effects.…”

Section: Resultsmentioning

confidence: 99%

“…Polygonum cuspidatum has been traditionally used for treatment of various inflammatory diseases, hepatitis, tumors and diarrhea (12). Recently, its extracts have been reported to possess the antiviral activity against hepatitis B virus (13), have potent estrogenic activities (14), inhibit bacterial DNA primase (15), and inhibit acyl-coenzyme A-cholesterol acyltransferase activity (16). Nevertheless, it is unknown whether Polygonum cuspidatum Sieb.…”

Section: Introductionmentioning

confidence: 99%

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Antioxidant activity of extract from Polygonum cuspidatum

Hsu¹,

Chan²,

Chang³

2007

Biol. Res.

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Numerous diseases are induced by free radicals via lipid peroxidation, protein peroxidation and DNA damage. It has been known that a variety of plant extracts have antioxidant activities to scavenge free radicals. Whether Polygonum cuspidatum Sieb. et Zucc has antioxidant activity is unknown. In this study, dried roots of Polygonum cuspidatum were extracted by ethanol and the extract was lyophilized. Free radical scavenging assays, superoxide radical scavenging assays, lipid peroxidation assays and hydroxyl radical-induced DNA strand scission assays were employed to study antioxidant activities. The results indicate that the IC50 value of Polygonum cuspidatum extract is 110 μg/ml in free radical scavenging assays, 3.2 μg/ml in superoxide radical scavenging assays, and 8 μg/ml in lipid peroxidation assays, respectively. Furthermore, Polygonum cuspidatum extract has DNA protective effect in hydroxyl radical-induced DNA strand scission assays. The total phenolics and flavonoid content of extract is 641.1 ± 42.6 mg/g and 62.3 ± 6.0 mg/g. The results indicate that Polygonum cuspidatum extract clearly has antioxidant effects.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Antioxidant activity of extract from Polygonum cuspidatum

Hsu¹,

Chan²,

Chang³

2007

Biol. Res.

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“…In contrast, A. aeolicus most likely evolved from Firmicutes and adapted to an environment with high temperatures by using initiation trinucleotides that are comprised of all guanines and cytosines (CGC>CCG), enhancing the stability of the DNA-RNA complex during the rate limiting primer synthesis step of dinucleotide formation. have lead to the identification of the enzyme as a target for novel antibiotic development (7,8).…”

Section: Discussionmentioning

confidence: 99%

New Therapeutic Strategies for Antibiotic-Resistant Select Agents

Hinrichs¹,

Griep²

2007

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The public reporting burden for this collection of information is estimated to average 1 hour per response, including the time for reviewing instructions, searching existing data sources, gathering and maintaining the data needed, and completing and reviewing the collection of information.Send comments regarding this burden estimate or any other aspect of this collection of information, including suggesstions for reducing this burden, to Washington Headquarters Services, Directorate for Information Operations and Reports, 1215 Jefferson Davis Highway, Suite 1204, Arlington VA, 22202-4302. Respondents should be aware that notwithstanding any other provision of law, no person shall be subject to any oenalty for failing to comply with a collection of information if it does not display a currently valid OMB control number. PLEASE DO NOT RETURN YOUR FORM TO THE ABOVE ADDRESS. a. REPORTNew Therapeutic Strategies for Antibiotic-Resistant Select Agents 14. ABSTRACT SECURITY CLASSIFICATION OF:The goal of this project was to establish the scientific basis for a new class of antibiotics that are needed to combat the development of resistance to currently available medications or the engineering of resistance into biological select agents. A multidisciplinary team of microbiologists, biochemists, molecular biologists and drug discovery experts was established to explore the hypothesis that disruption of bacterial primase activity will provide the basis for antimicrobial discovery and further, that the modular components of primase will provide for the discovery of broad-and narrow-spectrum antibiotics. The experimental model examined the functional activities of the Gram positive Staphylococcus aureus with the Gram negative E. S REPORT DATE (DD-MM-YYYY) TITLE AND SUBTITLE 31-12-200713. SUPPLEMENTARY NOTES The views, opinions and/or findings contained in this report are those of the author(s) and should not contrued as an official Department of the Army position, policy or decision, unless so designated by other documentation. Approved for public release; Federal purpose rights UL New Therapeutic Strategies for Antibiotic-Resistant Select AgentsReport Title ABSTRACTThe goal of this project was to establish the scientific basis for a new class of antibiotics that are needed to combat the development of resistance to currently available medications or the engineering of resistance into biological select agents. A multidisciplinary team of microbiologists, biochemists, molecular biologists and drug discovery experts was established to explore the hypothesis that disruption of bacterial primase activity will provide the basis for antimicrobial discovery and further, that the modular components of primase will provide for the discovery of broad-and narrow-spectrum antibiotics. The experimental model examined the functional activities of the Gram positive Staphylococcus aureus with the Gram negative E. coli for purposes of establishing a protocol for extension to select agents including Bacillus anthracis and Yersinia pestis. Impo...

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“…In our studies on Nepalese crude drugs, we isolated two new stilbenes (14,15) together with a known coumarin (1), a tyramine derivative (2), two pyrogallol derivatives (3, 4), four flavonoids (5)(6)(7)(8), five stilbenes (9-13), a flavan-3-ol (16), and two proanthocyanidins (17,18) from an extract of MeOH and an anthocyanin (19) from that of H 2 O-HCOOH (20 : 1) of the underground parts in this plant as described in Experimental. This paper deals with the identification of their structures.…”

mentioning

confidence: 99%

“…The structures of the known compounds were identified as 5,7-dihydroxycoumarin (1), 5) trans-feruloyltyramine (2), 6) gallic acid (3), 7) b-glucogallin (4), 7) trifolin (5), 8) hyperin (6), 9,10) myricetin 3-O-b-D-galactopyranoside (7), 11,12) myricitrin (8), 11) resveratorol (9), 13) astringenin (10), 14) piceid (11), 15) astringin (12), 16) resveratorol 3-O-b-D-(6-Ogalloyl)glucopyranoside (13), 17,18) (Ϫ)-epigallocatechin 3-Ogallate (16), 19) catechin-(4a→8)-epigallocatechin 3-O-gallate (17), 19) epicatechin 3-O-gallate-(4b→8)-epigallocatechin 3-O-gallate (18), 19) and idaein (19) 20) by direct comparison with authentic samples or of the respective spectral and chemical data with those described in the literature. Compounds 14 and 15 gave the IR absorption bands assignable to hydroxyl, conjugated carbonyl groups, and aromatic rings in IR spectra.…”

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confidence: 99%