Two new estrogen-supersensitive variants of the MCF-7 human breast cancer cell line

Natoli, Clara; Sica, Gigliola; Natoli, V.; Serra, Angelo; Iacobelli, Stéfano

doi:10.1007/bf01806231

Cited by 39 publications

(9 citation statements)

References 24 publications

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“…This increase was of the same order of magnitude as that described previously in monolayer cultures of MCF7 cells (5,(12)(13)(14)(15)(16).The other three cell stocks responded to estradiol-17f with a much smaller increase in cell yield, which was never higher than twofold over control values. Similar proliferative responses were reported in MCF7 cell stocks tested in media supplemented with different amounts of charcoal dextran serum, which ranged from 20% to 0.5% (17)(18)(19)(20)(21)(22)(23)(24)(25)(26)(27)(28)(29)(30), and in serumless medium (31)(32)(33). Poor proliferative responses to estradiol-17t3 were described when nonstripped, serum-supplemented media were used (34)(35)(36).…”

Section: Discussionsupporting

confidence: 72%

The E-screen assay: a comparison of different MCF7 cell stocks.

Villalobos

Olea

Brotons

et al. 1995

Environ Health Perspect

223

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For routine maintenance, cells were grown in Dulbecco's modification of Eagle's medium (DME) supplemented with 5% fetal bovine serum (FBS; PAA Labor und Forschungs Ges, MBH, Linz, Austria) in an atmosphere of 5% C02/95% air under saturating humidity at 37°C, except for MCF7 cells BB104, which were routinely maintained in 10% charcoal dextran-treated human serum (CDHS)-supplemented phenol red-free DME medium, prepared as described below.Plasma-derived human serum was prepared from expired plasma by adding calcium chloride to a final concentration of 30 mM to facilitate clot formation. Sex steroids were removed from serum by charcoal-dextran stripping (6).Cellproliferation experiments. We used MCF7 cells in the E-screen test according to a technique slightly modified from that originally described by Soto et al. (5). Briefly, cells were trypsinized and plated in 24-well plates (Limbro, McLean, Virginia) at an initial concentration of 10,000 cells per well in 5% FBS in DME. BB104 cells were seeded in 10% CDHS supplemented medium. The cells were allowed to attach for 24 hr, then 10% CDHS-supplemented phenol red-free DME was substituted for the seeding medium. A range of concentrations of the test compound were added, and the assay was stopped after 144 hr by removing the medium from wells, fixing the cells, and staining them with sulforhodamine-B (SRB).The fixation and staining technique was modified from that described by Skehan et al. (9). Briefly, cells were treated with cold 10% trichloracetic acid and incubated at 4°C for 30 min. Then the cells were washed five times with tap water and left to dry. The fixed cells were stained for 10 min with

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Section: Discussionsupporting

confidence: 72%

The E-screen assay: a comparison of different MCF7 cell stocks.

Villalobos

Olea

Brotons

et al. 1995

Environ Health Perspect

223

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“…Oestradiol has a growth promoting effect on oestrogen receptor positive human breast cancer cell lines (Lippman et al, 1976;Barnes & Sato, 1979;Allegra & Lippman, 1980;Chalbos et al, 1982;Leung et al, 1982;Darbre et al, 1983;Page et al, 1983;Dembinsky & Green, 1983;Natoli et al, 1983;Soto & Sonnenschein, 1984). However, the same cell line may be oestrogen responsive in one laboratory whereas it is nonresponsive in another laboratory (Butler et al, 1983;Iacobelli et al, 1984;Katzenellenbogen et al, 1984).…”

Section: Discussionmentioning

confidence: 99%

“…However, the same cell line may be oestrogen responsive in one laboratory whereas it is nonresponsive in another laboratory (Butler et al, 1983;Iacobelli et al, 1984;Katzenellenbogen et al, 1984). These differences may be due to different growth conditions and also divergence of the cell lines (Natoli et al, 1983;Katzenellenbogen et al, 1984). An understanding of the mechanism underlying the oestrogen stimulation of cell proliferation of human breast cancer cells may be of great importance for the treatment of oestrogen dependent human breast tumours.…”

Section: Discussionmentioning

confidence: 99%

Indirect mechanism of oestradiol stimulation of cell proliferation of human breast cancer cell lines

Lykkesfeldt¹,

Briand²

1986

Br J Cancer

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SummaryThe human breast cancer cell line MCF-7 requires oestrogen to produce and promote growth of tumours in athymic mice. In vitro, however, MCF-7 cells proliferate rapidly without supply of oestrogen (Briand & Lykkesfeldt, 1984). Oestrogen stimulation of proliferation of MCF-7 cells can be achieved when the cells are grown at high concentration of newborn calf serum (NCS, 10%) or oestrogen deprived foetal calf serum (10%). The stimulation involves an abolishment of inhibitory activity present in the serum. The oestradiol stimulated cultures grow rapidly for a much longer time period and attain a much higher cell density than the unstimulated cultures. Oestrogen is specific for the promotion of cell proliferation and only oestrogen receptor positive cell lines with a functional oestrogen receptor mechanism can be stimulated. We assume that oestradiol acts directly on the cells and via the oestrogen receptor mechanism induces the synthesis of a substance which abolishes the inhibitory activity in serum. We call this mechanism of action an indirect stimulation of cell proliferation. A similar mechanism may exist in vivo since we find that serum from athymic mice contains a growth inhibitory activity towards MCF-7 cells and the inhibitory effect can be abolished by oestradiol.

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“…was previously described [5]. The other human breast cancer cell lines are from The American Tissue Culture Collection.…”

Section: Methodsmentioning

confidence: 99%

Purification and characterization of a 90 kDa protein released from human tumors and tumor cell lines

Iacobelli¹,

Bucci²,

D'Egidio³

et al. 1993

FEBS Letters

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A novel tumor-associated protein, termed 90K, and recognized by mAb SP-2 was purified from serum of breast cancer patients, ovarian cancer a&tic fluid and conditioned medium of human breast cancer cells. In these three sources, native 90K is present as a high molecular weight complex that was dissociated by SDS-PAGE into a major band of approximately 90,000 Da. On the basis of electrophoretic mobility, buoyant density value, amino acid composition, and immunoreactivity, the 90K from the different sources appeared to be identical. NH*-terminal amino acid sequence revealed no homology to known protein.Tumor-associated antigen; Monoclonal antibody; Breast cancer; Protein purification; NH,-terminal amino acid sequence

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Two new estrogen-supersensitive variants of the MCF-7 human breast cancer cell line

Cited by 39 publications

References 24 publications

The E-screen assay: a comparison of different MCF7 cell stocks.

The E-screen assay: a comparison of different MCF7 cell stocks.

Indirect mechanism of oestradiol stimulation of cell proliferation of human breast cancer cell lines

Purification and characterization of a 90 kDa protein released from human tumors and tumor cell lines

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