Two new growth inhibition tests with the filamentous algae Ceramium strictum and C. tenuicorne (Rhodophyta)

Bruno, Ellen; Eklund, Britta

doi:10.1016/s0269-7491(03)00050-2

Cited by 20 publications

(8 citation statements)

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“… From Bruno and Eklund (2003) and Eklund (1995). Natural levels of nitrate and phosphate in the filtered seawater used were 8.1 μM N and 0.08 μM P. Data on the background values of trace elements were not obtainable.…”

Section: Methodsmentioning

confidence: 99%

“…This time period may be too short for speciation to occur, but ecotypic adaptations to the brackish environment have been observed in many Baltic organisms in comparison to their marine counterparts, including nearly all marine macroalgae studied (Russell 1985, 1988, Thomas et al 1990, Rietema 1991, Rueness and Kornfeldt 1992, Rietema 1993, Kristiansen et al 1994, Russell 1994, Serrão et al 1996, Düwel 2001). However, it is also seen that Baltic organisms are subject to continuous physiological stress in the low salinity, which negatively affects their tolerance of further stress‐factors such as pollution (Andersson et al 1992, Andersson and Kautsky 1996, Bruno and Eklund 2003). Evolutionary processes are related not only to scale of selection, but also to the scale of dispersal of organisms (Lenormand 2002, Johannesson 2003).…”

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confidence: 99%

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LOCAL ADAPTATION OF CERAMIUM TENUICORNE (CERAMIALES, RHODOPHYTA) WITHIN THE BALTIC SEA¹

Bergström

Kautsky

2006

Journal of Phycology

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Ceramium tenuicorne (Kützing) Waern is a red alga that is widely distributed in the brackish Baltic Sea. We studied the growth response of Ceramium to low salinity and nutrient enrichment, using isolades from two regions of the Baltic Sea where the alga approaches its inner distribution limit. Ecotypic differentiation was observed in that differences in growth response among isolates corresponded to salinity conditions in their regions of origin. Isolates from the Gulf of Bothnia (4 psu) had inherently lower growth rates that were not increased when transferred to higher salinity, but were better adapted to very low salinity levels than isolates from the Baltic Proper (7 psu). Further, the results indicate that Ceramium from different regions of the Baltic Sea vary in their responses to nutrient enrichment. The observed differences may be best described as a quantitative difference in the proportion of isolates with hyposaline adaptation. The results indicate that the wide distribution of Ceramium in the Baltic Sea is better explained by the occurrence of locally adapted genotypes than by a generalist life strategy, and provide example of adaptive differentiation in a marine edge environment.

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Section: Methodsmentioning

confidence: 99%

mentioning

confidence: 99%

LOCAL ADAPTATION OF CERAMIUM TENUICORNE (CERAMIALES, RHODOPHYTA) WITHIN THE BALTIC SEA¹

Bergström

Kautsky

2006

Journal of Phycology

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“…The brackish water clone used in this study is common in the Baltic Sea (Bergström et al 2004). The principle of the test is to determine growth inhibition as reduction in growth rate, measured as length at different concentrations of chemical/ effluent water compared to a control during a 7-day exposure period (Bruno and Eklund 2003;Eklund 2005;ISO 2008). The test has been used in many applications, e.g.…”

Section: Whole Sediment and Leachate Tests With C Tenuicorne-growth mentioning

confidence: 99%

Biological and chemical characterization of harbour sediments from the Stockholm area

et al. 2009

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Purpose The main objective of the current study was to assess the impact of pleasure boat activities on harbour sediment quality in the Stockholm area. Sediment contamination is a growing ecological issue, and there is consequently a need to use sediment bioassays in combination with chemical analysis to determine the impact on the ecosystem. To generate sediment toxicity data relevant for the Baltic Sea, a secondary objective was to further develop and evaluate two well-established bioassays for saltwater, with the macroalga Ceramium tenuicorne and the crustacean Nitocra spinipes, to be useful also for toxicity testing of whole sediment. A major concern has been to minimize any manipulation of the sediments. A third objective was to assess whether a simple leaching procedure could be used to simulate sediment toxicity by comparing results from whole sediment and leachate tests. Materials and methods Surface sediments (0-2 cm) from five different types of pleasure boat harbours were collected. Chemical measurements of boat related compounds, i.e. tin organic substances (tributyltin (TBT), dibutyl tin, and monobutyl tin), polyaromatic hydrocarbons (PAHs), copper, zinc, lead, tin and irgarol were conducted. The sediments were tested for toxic effects using the established Microtox® test as well as the developed sediment tests with C. tenuicorne and N. spinipes. The endpoints are growth inhibition for the algal test and for the crustacean test mortality of larvae and rate of development expressed as the ratio between nauplia and copepodites. Two different procedures have been compared with both organisms, i.e. exposure to whole sediment and to leachate. The duration of both tests is around 1 week. Results and discussion All harbour locations were more or less heavily contaminated with remnants from use of antifouling paints. The sediment in a smaller marina (ca. 250 boats) contained the highest levels of TBT (max 1,400 µg/kg dry weight (dw)), whereas the centre of Stockholm City had the highest concentrations of all measured metals (max values Cu 252, Pb 830, Sn 25 and Zn 600 µg/kg dw) as well as high concentrations of total PAH (18 mg/kg dw). All three organisms were well suited to test the toxicity of contaminated sediments and were able to discriminate between more or less polluted sediments. The sediments in the smaller marina were most toxic along with sediments adjacent to slipways and sediments from the centre of Stockholm. No significant difference was found between the two different procedures for the algal tests. The whole sediment test was significantly more toxic to N. spinipes than the test using leachate. Conclusions Our results show that in spite of prohibition for many years to use tin organic substances and copper, boat activities still contribute with high concentrations of these toxic substances from anti-fouling paints in the surface sediment and that these have effects on organisms normally living in this environment. The present study has also demonstrated an expansion of two well-established t...

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“…Within laboratory variability (CV) in biological test methods is often 20%-40% (Bruno and Eklund 2003). The coefficients of variation (CV) for this test's 24 h LC50 were *15%, and there was no statistically significant difference between observed mortality between any of the consistency tests (p [ 0.05), i.e.…”

Section: Resultsmentioning

confidence: 90%

Assessing the Performance of a Bdelloid Rotifer Philodina acuticornis odiosa Acute Toxicity Assay

Hagen

Allinson²,

Wightwick³

et al. 2008

Bull Environ Contam Toxicol

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A bioassay using the bdelloid rotifer, Philodina acuticornis odiosa, was evaluated for use as a standard test method for direct toxicity assessment testing in the Australasian region. Philodina acuticornis odiosa was found to be relatively tolerant to phenol (24 h LC50, 142 mg/L). The mortality endpoint was both reliable and repeatable (the coefficients of variation for mortality at the 24 h LC50 concentration ranged from 11%-24% (n = 8)), sufficiently low to warrant further testing with a range of reference toxicants, so that this organism can be included for use as a regulatory test in Australasia.

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Two new growth inhibition tests with the filamentous algae Ceramium strictum and C. tenuicorne (Rhodophyta)

Cited by 20 publications

References 24 publications

LOCAL ADAPTATION OF CERAMIUM TENUICORNE (CERAMIALES, RHODOPHYTA) WITHIN THE BALTIC SEA¹

LOCAL ADAPTATION OF CERAMIUM TENUICORNE (CERAMIALES, RHODOPHYTA) WITHIN THE BALTIC SEA¹

Biological and chemical characterization of harbour sediments from the Stockholm area

Assessing the Performance of a Bdelloid Rotifer Philodina acuticornis odiosa Acute Toxicity Assay

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Two new growth inhibition tests with the filamentous algae Ceramium strictum and C. tenuicorne (Rhodophyta)

Cited by 20 publications

References 24 publications

LOCAL ADAPTATION OF CERAMIUM TENUICORNE (CERAMIALES, RHODOPHYTA) WITHIN THE BALTIC SEA1

LOCAL ADAPTATION OF CERAMIUM TENUICORNE (CERAMIALES, RHODOPHYTA) WITHIN THE BALTIC SEA1

Biological and chemical characterization of harbour sediments from the Stockholm area

Assessing the Performance of a Bdelloid Rotifer Philodina acuticornis odiosa Acute Toxicity Assay

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Product

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LOCAL ADAPTATION OF CERAMIUM TENUICORNE (CERAMIALES, RHODOPHYTA) WITHIN THE BALTIC SEA¹

LOCAL ADAPTATION OF CERAMIUM TENUICORNE (CERAMIALES, RHODOPHYTA) WITHIN THE BALTIC SEA¹