Two new species of Diaporthe (Diaporthaceae, Diaporthales) associated with tree cankers in the Netherlands

Abstract

Diaporthe (Diaporthaceae, Diaporthales) is a common fungal genus inhabiting plant tissues as endophytes, pathogens and saprobes. Some species are reported from tree branches associated with canker diseases. In the present study, Diaporthe samples were collected from Alnus glutinosa, Fraxinus excelsior and Quercus robur in Utrecht, the Netherlands. They were identified to species based on a polyphasic approach including morphology, pure culture characters, and phylogenetic analyses of a combined matrix of partial ITS, cal, his3, tef1 and tub2 gene regions. As a result, four species (viz. Diaporthe pseudoalnea sp. nov. from Alnus glutinosa, Diaporthe silvicola sp. nov. from Fraxinus excelsior, D. foeniculacea and D. rudis from Quercus robur) were revealed from tree branches in the Netherlands. Diaporthe pseudoalnea differs from D. eres (syn. D. alnea) by its longer conidiophores. Diaporthe silvicola is distinguished from D. fraxinicola and D. fraxini-angustifoliae by larger alpha conidia.

Pseudocryphonectria elaeocarpicola gen. et sp. nov. (Cryphonectriaceae, Diaporthales) causing stem blight of Elaeocarpus spp. in China

“…In the fungal order Diaporthales, many species were reported as forest pathogens causing leaf spots, cankers, fruit rot or blight diseases (Visentin et al 2012;Pasche et al 2016;Shuttleworth and Guest 2017;Jiang et al 2021b;Pan et al 2021;Lin et al 2022), moreover, cryphonectriaceous members are known to be serious pathogens (Chen et al 2013(Chen et al , 2016Beier et al 2015;Ferreira et al 2019;Wang et al 2020). This family is easily recognized based on the disease symptoms and their obvious orange conidioma formed on the cankered barks, together with their hyaline and small (Gryzenhout et al 2006).…”

Three New Species of Microdochium (Sordariomycetes, Amphisphaeriales) on Miscanthus sinensis and Phragmites australis from Hainan, China

“…The strains of Microdochium were isolated from diseased leaves of Miscanthus sinensis and Phragmites australis using a tissue-isolation method [ 25 ]. Tissue fragments (5 × 5 mm) were taken from the margin of leaf lesions and surface-sterilized by immersing consecutively in 75% ethanol solution for 1 min, 5% sodium hypochlorite solution for 30 s, and then rinsing in sterile distilled water for 1 min [ 26 , 27 ]. The sterilized leaf fragments were dried with sterilized paper towels and placed on potato-dextrose agar (PDA) [ 28 ].…”

Studies of Diaporthe species causing hazelnut canker disease in Beijing, China, with two new species described

“…The genus Diaporthe comprises endophytic fungal pathogens that infect the leaves, branches and seeds of plants (Gao et al, 2021;Jiang et al, 2021;Murali et al, 2006;Udayanga et al, 2011;Yang et al, 2017Yang et al, , 2018Yang et al, , 2020. Over 1150 species of Diaporthe have been recorded in the Index Fungorum (www.index fungo rum.org; accessed on 4 August 2022).…”

“…Although morphological identification has been used to determine the species of Diaporthe (van der Aa et al, 1990), this is unsatisfactory for accurate identification when used alone (Duan et al, 2016). Therefore, currently, a polyphasic taxonomic approach, which combines phylogenetic and morphological analyses, is widely employed (Gao et al, 2021; Gomes et al, 2013; Jiang et al, 2021; Udayanga et al, 2014; Yang et al, 2020). Five genetic sequences are widely used for phylogenetic analyses: nuclear ribosomal internal transcribed spacer (ITS), calmodulin ( cal ), histone H3 ( his3 ), translation elongation factor 1‐α ( tef1‐α ), and β‐tubulin ( tub2 ) (Dissanayake et al, 2020; Gao et al, 2017, 2021; Jiang et al, 2021).…”