This paper reports three new species of the genus Nigrimacula Shi, Bian & Zhou, 2016 from Yunnan, China, i.e. Nigrimacula biprotubera sp. nov., Nigrimacula trigona sp. nov. and Nigrimacula wuliangshanensis sp. nov. In addition, we supplement the male description of Nigrimacula inlobata Cui, Liu & Shi, 2021.