Two new standard reference materials for the determination of drugs of abuse in human hair

Welch, Michael J.; Sniegoski, Lorna T.; Tai, Susan S.-C.

doi:10.1007/s00216-003-2061-4

Cited by 49 publications

(28 citation statements)

References 10 publications

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“…Hair samples were fortified by soaking using a previously reported procedure [38]. Briefly, hair were cut and immersed in a flask containing a solution consisting of 1.5 mL of HCl (0.02 M in dimethyl sulfoxide (DMSO)) and 1.5 mL of water containing the analytes.…”

Section: Hair Extractionmentioning

confidence: 99%

Selective solid phase extraction of JWH synthetic cannabinoids by using computationally designed peptides

Mascini

Montesano

Pérez

et al. 2017

Talanta

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A B S T R A C TThe objective of the present work is to demonstrate a rational way to prepare selective sorbents able to extract simultaneously several structural analogs. For this purpose the binding specificity of two hexapeptides computationally designed (VYWLVW and YYIGGF) versus four synthetic cannabinoids Naphthalen-1-yl-(1-pentylindol-3-yl)methanone (JWH 018), naphthalen-1-yl-(1-butylindol-3-yl)methanone (JWH 073), (R)-(1-((1-methylpiperidin-2-yl)methyl)-1H-indol-3-yl)(naphthalen-1-yl)methanone (AM 1220) and (R)-(+)-[2,3-Dihydro-5-methyl-3-(4-morpholinylmethyl)pyrrolo[1,2,3-de]-1,4-benzoxazin-6-yl]-1-napthalenylmethanone (WIN 55) was computationally studied and then experimentally tested by solid-phase extraction (SPE) clean-up and ultra-high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) analysis. The two peptides were chosen using a semi combinatorial virtual technique by generating 4 cycles of peptide libraries (around 2.3×10 4 elements). To select the two peptides, the simulated binding scores between synthetic cannabinoids and peptides was used by maximizing the recognition properties of amino acid motif between the two JWH and the other synthetic cannabinoids. In particular, the peptide YYIGGF, having also affinity for AM 120, was selected as control because it was the only one without tryptophan residues within the best peptides obtained from simulation. Experimentally, the two hexapeptides were tested as SPE sorbent using nanomolar solutions of the four drugs. After optimization of best retentions the binding constants were calculated by loading synthetic cannabinoids solutions at different concentrations. The results indicated a strong interaction between hexapeptide VYWLVW and JWH 018 (15.58 ± 2.03×10 6 M -1 ), 3-fold and 40-fold larger compared to the analog JWH 073 and both AM 1220 and the WIN 55. Similar trend was observed for the hexapeptide YYIGGF but the binding constants were at least three times lower highlighting the key role of the tryptophan. To demonstrate the hexapeptides specific interaction with only synthetic cannabinoids, a cross-reactivity study was carried out using other drugs (cocaine, morphine, phencyclidine and methamphetamine) in the same SPE condition. Finally the practical utility of these peptide modified sorbent materials was further demonstrated by detecting the synthetic cannabinoids in real samples using hair matrix.

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Section: Hair Extractionmentioning

confidence: 99%

Selective solid phase extraction of JWH synthetic cannabinoids by using computationally designed peptides

Mascini

Montesano

Pérez

et al. 2017

Talanta

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“…The hair RM was prepared using drug-free hair as previously described [10]. About 10 g of drug-free hair was shampooed and washed with a sufficient volume of water, methanol and dichloromethane twice for each and used for the preparation of the RM after air-dried and cut into about 5 cm.…”

Section: Preparation Of a Rmmentioning

confidence: 99%

“…[3,4]. Moreover, as the number of cases requiring hair testing has been increased, its related issues -development of sensitive analytical methods, interpretation of results, quality control, characteristics of hair itself, for example -have been discussed in detail [1,[4][5][6][7][8][9][10][11].…”

Section: Introductionmentioning

confidence: 99%

Preparation and application of a fortified hair reference material for the determination of methamphetamine and amphetamine

Lee¹,

Park²,

Han³

et al. 2008

Forensic Science International

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“…The former consists of hair spiked with cocaine, benzoylecgonine, cocaethylene, phencyclidine, amphetamine (AP) and methamphetamine (MA). The latter consists of hair spiked with codeine, morphine, monoacetylmorphine and tetrahydrocannabinol (Welch et al, 2003). Nevertheless, the Society of Hair Testing (SoHT) recommended that authentic standard hair specimens be used in a proficiency testing program for external quality control (SoHT, Accessed 5 Oct 2010) because the condition of drug incorporation of the spiked hair is different from that in real cases.…”

Section: Introductionmentioning

confidence: 99%

Feasibility of rat hair as a quality control material for the determination of methamphetamine and amphetamine in human hair

et al. 2011

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A quality control material (QCM) is a necessity in hair drug analysis, but it is not always easy to have an authentic hair sample containing various target drugs and metabolites. In the present study, the feasibility of rat hair as a QCM was examined for its application in the determination of methamphetamine (MA) and amphetamine (AP) in human hair. MA was administered to lean Zucker rats, from which only pigmented hair was collected for the preparation of a QCM. The rat hair was then washed, homogenized and finally bottled. Both homogeneity and stability were examined in order to demonstrate the suitability of rat hair as a QCM in hair drug analysis. The concentrations of MA and AP in each bottle were determined using extraction with 1% HCl in methanol at 38°C followed by gas chromatography/mass spectrometry after derivatization with trifluoroacetic anhydride. Furthermore, the prepared QCM was used in an inter-laboratory quality assurance program. In the homogeneity test, no significant difference was observed between bottles of the QCM. The statistical results also showed no significant trends in stability for three months at room temperature. An inter-laboratory quality assurance program was also performed successfully using this material. Thus, rat hair will be useful as an alternative QCM sample for the determination of a variety of drugs and their metabolites in human hair.

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Two new standard reference materials for the determination of drugs of abuse in human hair

Cited by 49 publications

References 10 publications

Selective solid phase extraction of JWH synthetic cannabinoids by using computationally designed peptides

Selective solid phase extraction of JWH synthetic cannabinoids by using computationally designed peptides

Preparation and application of a fortified hair reference material for the determination of methamphetamine and amphetamine

Feasibility of rat hair as a quality control material for the determination of methamphetamine and amphetamine in human hair

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