Two novel blaNDM-1-harbouring transposons on pPrY2001-like plasmids coexisting with a novel cfr-encoding plasmid in food animal source Enterobacteriaceae

Zheng, Xing-Run; Sun, Yin-Huan; Zhu, Jia-Hang; Wu, Si-Li; Cai, Peng; Fang, Liang‐Xing; Jiang, Hongxia

doi:10.1016/j.jgar.2021.06.006

Cited by 7 publications

(6 citation statements)

References 18 publications

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“…Different from previous studies often associated bla NDM-1 with Tn125, especially ISAba125 (Nordmann et al, 2011;Poirel et al, 2011a;Nordmann et al, 2012), in our study, was TnAs3, which is relatively rare reported. As to insertion sequences, IS26 is widely distributed and it is often combined with Tn125 family transposons (Poirel et al, 2011a;Zheng et al, 2021), in P. rettgeri isolate P138, bla NDM-1 was associated at its 3'-end and 5' -end with IS110 that is also relative rare reported. This further reflects the diversity of genetic elements, which leads to the wide spread of resistance genes among bacteria.…”

Section: Discussionmentioning

confidence: 99%

Occurrence of NDM-1, VIM-1, and OXA-10 Co-Producing Providencia rettgeri Clinical Isolate in China

Shen

Huang

Shi

et al. 2022

Front. Cell. Infect. Microbiol.

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Providencia rettgeri is a nosocomial pathogen associated with urinary tract infections related to hospital-acquired Infections. In recent years, P. rettgeri clinical strains producing New Delhi Metallo-β-lactamase (NDM) and other β-lactamase which reduce the efficiency of antimicrobial therapy have been reported. However, there are few reports of P. rettgeri co-producing two metallo-β-lactamases in one isolate. Here, we first reported a P. rettgeri strain (P138) co-harboring blaNDM-1, blaVIM-1, and blaOXA-10. The specie were identified using MALDI-TOF MS. The results of antimicrobial susceptibility testing by broth microdilution method indicated that P. rettgeri P138 was resistant to meropenem (MIC = 64μg/ml), imipenem (MIC = 64μg/ml), and aztreonam (MIC = 32μg/ml). Conjugation experiments revealed that the blaNDM-1-carrying plasmid was transferrable. The carbapenemase genes were detected using PCR and confirmed by PCR-based sequencing. The complete genomic sequence of the P. rettgeri was identified using Illumina (Illumina, San Diego, CA, USA) short-read sequencing (150bp paired-end reads), and many common resistance genes had been identified, including blaNDM-1, blaVIM-1, blaOXA-10, aac(6’)-Il, aadA5, ant(2’’)-Ia, aadA1, aac(6’)-Ib3, aadA1, aph(3’)-Ia, aac(6’)-Ib-cr, qnrD1, qnrA1, and catA2. The blaNDM-1 gene was characterized by the following structure: IS110–TnpA–IntI1–aadB–IS91–GroEL–GroES–DsbD–PAI–ble–blaNDM-1–IS91–QnrS1–IS110. Blast comparison revealed that the blaNDM-1 gene structure shared >99% similarity with plasmid p5_SCLZS62 (99% nucleotide identity and query coverage). In summary, we isolated a P. rettgeri strain coproducing blaNDM-1, blaVIM-1, and blaOXA-10. To the best of our acknowledge, this was first reported in the world. The occurrence of the strain needs to be closely monitored.

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Section: Discussionmentioning

confidence: 99%

Occurrence of NDM-1, VIM-1, and OXA-10 Co-Producing Providencia rettgeri Clinical Isolate in China

Shen

Huang

Shi

et al. 2022

Front. Cell. Infect. Microbiol.

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“…However, since the number of sequenced isolates was limited, further investigations that include a higher number of M. wisconsensis are needed to confirm these observations and to assess the molecular features and potential role of these megaplasmids in the dissemination of antimicrobial resistance determinants in wildlife and elsewhere. By contrast, the bla CTX-M-32 carrying plasmid pW17–3-a identified in M. wisconsensis was related to Proteus mirabilis plasmid pJPM24 (query coverage: 86%, identity: 99.95%) isolated from chicken faeces in China (NZ_CP053895.1) [ 31 ], and the bla CTX-M-32 harbouring plasmid pW49–2-b from E. marmotae was identical with pWE_H_2, a multidrug resistance plasmid which was found among a complex bacterial community taken from wastewater effluent in Sweden MW574948.1 [ 32 ], indicating wide dissemination of these ESBL-encoding plasmids.…”

Section: Discussionmentioning

confidence: 99%

Finding of extended-spectrum beta-lactamase (ESBL)-producing Enterobacterales in wild game meat originating from several European countries: predominance of Moellerella wisconsensis producing CTX-M-1, November 2021

et al. 2022

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Introduction Meat can be a vehicle for food-borne transmission of antimicrobial resistant bacteria and antimicrobial resistance genes. The occurrence of extended‐spectrum beta‐lactamase (ESBL) producing Enterobacterales has been observed in meat from livestock production but has not been well studied in meat from wild game. Aim We aimed to investigate, particularly in central Europe, to what extent ESBL-producing Enterobacterales may be present in wild game meat. Methods A total of 111 samples of different types of game meat supplied by butchers, hunters, retail stores and a large game-processing establishment in Europe were screened for ESBL-producing Enterobacterales using a selective culture medium. Isolates were genotypically and phenotypically characterised. Results Thirty-nine samples (35% of the total) yielded ESBL-producing Enterobacterales, with most (35/39) supplied by the game-processing establishment. Isolates included 32 Moellerella wisconsensis, 18 Escherichia coli and one Escherichia marmotae. PCR screening identified bla CTX-M-1 (n = 31), bla CTX-M-32 (n = 8), bla CTX-M-65 (n = 4), bla CTX-M-15 (n = 3), bla CTX-M-8 (n = 1), bla CTX-M-14 (n = 1), bla CTX-M-55 (n = 1), and bla SHV-12 (n = 2). Most E. coli belonged to phylogenetic group A (n = 7) or B1 (n = 9), but several isolates belonged to extraintestinal pathogenic E. coli (ExPEC) sequence types (ST)58 (n = 4), ST68 (n = 1) and ST540 (n = 1). Whole genome sequencing of six selected isolates localised bla CTX-M-1 on megaplasmids in four M. wisconsensis and bla CTX-M-32 on IncN_1 plasmids in one M. wisconsensis and one E. marmotae. Forty-eight isolates (94%) exhibited a multidrug-resistance phenotype. Conclusion We found a high occurrence of ESBL-producing Enterobacterales in wild game meat, suggesting wildlife habitat pollution and possible microbial contamination events occurring during skinning or cutting carcasses.

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“…Current evidence suggests it is an opportunistic pathogen that can cause urinary tract, reproductive tract, skin and catheterrelated infections, etc [1]. P. mirabilis is naturally resistant to various antimicrobial drugs, including tigecycline and polymyxin [2], with reports suggesting increasing resistance to fluoroquinolones, aminoglycosides, macrolides and cephalosporins. In recent years, outbreaks of severe nosocomial infections caused by carbapenem resistance have been frequently reported, suggesting that multiple resistant bacteria, especially P. mirabilis, represent an important source of nosocomial infectious disease.…”

Section: Introductionmentioning

confidence: 99%

“…mirabilis mainly include reduced outer membrane permeability and carbapenemase production, which are mainly commonly detected in bla KPC , bla NDM , bla VIM , bla IMP and bla OXA , especially bla NDM [3, 4 ]. The bla NDM-1 gene was first described in Klebsiella pneumoniae in a patient returning from India to Sweden in 2008 and has recently been identified in numerous environmental and clinical Gram-negative bacteria [2]. It has been established that most of these carbapenem resistance genes are found on the resistant plasmids, and the backbone conserved regions of these resistant plasmids can simultaneously carry the resistance genes of various other antimicrobial drugs, which is also key to forming multiple drug resistance in P.…”

Section: Introductionmentioning

confidence: 99%

“…In 2019, the US CDC listed carbapenem-resistant Enterobacteriaceae (CRE) as an emergency threat [5], suggesting that CRE poses a great threat to human health [6] and is a major economic burden. Over the years, carbapenem-resistant P. mirabilis cases have been extensively reported in the literature [1,2,7,8]. Moreover, its natural resistance to various drugs has brought great challenges to clinical treatment, substantiating that this pathogen represents a great threat to public health [9].…”

Section: Introductionmentioning

confidence: 99%

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Study of the molecular characteristics and homology of carbapenem-resistant Proteus mirabilis by whole genome sequencing

Liu

Wei

et al. 2023

Journal of Medical Microbiology

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Introduction. Proteus mirabilis is part of the family Enterobacteriaceae , and is naturally resistant to various antimicrobial drugs. In recent years, outbreaks of severe nosocomial infections caused by carbapenem-resistant P. mirabilis (CR-PMI) have been frequently reported. Few studies exist on the whole-genome molecular characteristics of this bacterium in China and elsewhere, which stimulated the implementation of this study. Hypothesis. CR-PMI strains contained the multiple drug resistance genes and exhibited a high resistance rate to commonly used antimicrobial drugs. Aim. Our goals here were to identify resistance mechanisms and homology of CR-PMI strains and provide a theoretical basis for clinical treatment and controlling nosocomial infections. Methodology. Bacterial species identification was carried out using matrix-assisted laser desorption/ionization time of flight MS (MALDI-TOF-MS). Antimicrobial susceptibility was determined using the VITEK 2 system and Kirby-Bauer (K-B) disc-diffusion method. Whole-genome sequencing (WGS) was conducted by the Illumina platform NovaSeq sequencer. Antibiotic resistance genes (ARGs) were identified using the NCBI database with Abricate. Plasmid replicon types were identified using PlasmidFinder, available at the Center for Genomic Epidemiology. Results. Five CR-PMI strains collected in our hospital from July 2019 to September 2021 were resistant to almost all antimicrobial agents except aztreonam (ATM), amikacin (AMK) and cefotetan (CTT). All CR-PMI strains contained the carbapenem resistance gene New Delhi metallo-β-lactamase 1 (bla NDM-1), and two strains harboured extended-spectrum β-lactamase (ESBL) genes bla PER-4 and bla CTX-M-65. The five CR-PMI strains contained 27, 18, 30, 25 and 24 drug-resistance genes, respectively. Most antimicrobial resistance genes were detected for aminoglycosides (n=14), followed by cephalosporins (n=7). The phylogenetic tree was divided into five evolutionary groups, and the five CR-PMI strains were in the four evolutionary groups B–E. Conclusion Overall, CR-PMI strains exhibited a high resistance rate to commonly used antimicrobial drugs, and contained the carbapenem resistance gene bla NDM-1. The CR-PMI strains showed a polyclonal trend in different wards at different times. Most importantly, all strains identified contained important antimicrobial resistance genes, which may lead to severe drug resistance transmission and fatal multiple resistant bacterial infections.

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Two novel blaNDM-1-harbouring transposons on pPrY2001-like plasmids coexisting with a novel cfr-encoding plasmid in food animal source Enterobacteriaceae

Cited by 7 publications

References 18 publications

Occurrence of NDM-1, VIM-1, and OXA-10 Co-Producing Providencia rettgeri Clinical Isolate in China

Occurrence of NDM-1, VIM-1, and OXA-10 Co-Producing Providencia rettgeri Clinical Isolate in China

Finding of extended-spectrum beta-lactamase (ESBL)-producing Enterobacterales in wild game meat originating from several European countries: predominance of Moellerella wisconsensis producing CTX-M-1, November 2021

Study of the molecular characteristics and homology of carbapenem-resistant Proteus mirabilis by whole genome sequencing

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