Two novel Lipomycetaceous yeast species, Lipomyces okinawensis sp. nov. and Lipomyces yamanashiensis f.a., sp. nov., isolated from soil in the Okinawa and Yamanashi prefectures, Japan

Abstract

Four novel Lipomyces strains were isolated from soil collected in the Okinawa and Yamanashi prefectures, Japan. Based on their morphological and biochemical characteristics, along with sequence typing using the D1/D2 domain of the LSU rRNA, internal transcribed spacer (ITS) region including 5.8S rRNA, and translation elongation factor 1 alpha gene (EF-1α), the four strains were shown to represent two novel species of the genus Lipomyces, described as Lipomyces okinawensis sp. nov. (type strain No.3-a(35)T=NBRC 110620T=CBS 14747T) and Lipomyces yamanashiensis f.a., sp. nov. (type strain No.313T=NBRC 110621T=CBS 14748T).

North-to-South diversity of lipomycetaceous yeasts in soils evaluated with a cultivation-based approach from 11 locations in Japan

“…Lipomyces okinawensis was proposed for the isolates from Okinawa, which belongs to the subtropical zone in Japan ( Yamazaki, Yanagiba, & Naganuma, 2017b ). However, in this study, strains of this species were not isolated from Iriomote Island, Okinawa, but from Gosen-shi, Niigata Prefecture, a colder region ( Table 2 ).…”

“…The species L. japonicus , L. kalimantanensis , L. kockii , L. maratuensis , L. orientalis , and L. tropicalis were not isolated in this study. Lipomyces kalimantanensis , L. maratuensis , and L. tropicalis may be endemic to the areas from Kalimantan Island and Maratua Island, which is located in the east of Kalimantan Island, Indonesia, since isolation from other locations has not yet been reported ( Di Menna, 1966 ; Babjeva &Gorin, 1987 ; Thanh, 2006 ; Smith & Kurtzman, 2011 ; Yamazaki & Kawasaki, 2014 ; Yamazaki et al, 2017a , b , 2020 ).…”

“…Species of the genus Lipomyces cannot be clearly differentiated by commonly used phylogenetic criteria, such as the sequence of the D1/D2 domain of LSU rDNA and internal transcribed spacers (ITSs), including 5.8S rDNA regions. However, our previous study revealed that the translation elongation factor 1 alpha gene ( TEF1 , also known as eEF1A and EF-1α ) was useful for species identification and classification of the family Lipomycetaceae ( Yamazaki & Kawasaki, 2014 ; Yamazaki, Kanti, & Kawasaki, 2017a ; Yamazaki, Yanagiba, & Naganuma, 2017b ; Yamazaki, Lorliam, Kawasaki, Uchino, & Suzuki, 2020 ).…”

“…Lipomycetaceous yeasts have been isolated from various soils worldwide and form watery mucoid-like colonies ( Di Menna, 1966 ; Babjeva & Gorin, 1987 ; Phaff & Starmer, 1987 ; Thanh, 2006 ; Smith & Kurtzman, 2011 ; Yamazaki & Kawasaki, 2014 ; Yamazaki et al, 2017a , 2017b , 2020 ) (Supplementary Table S1). Currently, the genus Lipomyces consists of 30 species isolated from soil, including species proposed in our recent studies ( Yamazaki et al, 2017a , 2017b , 2020 ).…”

“…Lipomycetaceous yeasts have been isolated from various soils worldwide and form watery mucoid-like colonies ( Di Menna, 1966 ; Babjeva & Gorin, 1987 ; Phaff & Starmer, 1987 ; Thanh, 2006 ; Smith & Kurtzman, 2011 ; Yamazaki & Kawasaki, 2014 ; Yamazaki et al, 2017a , 2017b , 2020 ) (Supplementary Table S1). Currently, the genus Lipomyces consists of 30 species isolated from soil, including species proposed in our recent studies ( Yamazaki et al, 2017a , 2017b , 2020 ). Some of the soil yeasts including the genera Aureobasidium, Burella, Candida, Cryptococcus, Lipomyces , Pichia, Rhodotorula, Sporobolomyces, Tremella , and Trichosporon produce extracellular polysaccharides (EPS), which prevent them from desiccation, UV radiation, and predation, and are considered to play important role in soil aggregation and stability by improving the water retention capacity and fertility of soil ( Botha, 2006 , 2011 ; van Bogaert, De Maeseneire, & Vandamme, 2009 ; Yurkov, 2017 , 2018 ).…”