The image segmentation is the most important operation in an image processing system. It is located at the joint between the processing and analysis of the images. Unsupervised segmentation aims to automatically separate the image into natural clusters. However, because of its complexity several methods have been proposed, specifically methods of optimization. In our work we are interested to the technique SFLA (Shuffled Frog-Leaping Algorithm). It's a memetic meta-heuristic algorithm that is based on frog populations in nature searching for food. This paper proposes a new approach of unsupervised image segmentation based on SFLA method. It is implemented and applied to different types of images. To validate the performances of our approach, we performed experiments which were compared to the method of K-means.