Two Phosphate- and Potassium-solubilizing Bacteria Isolated from Tianmu Mountain, Zhejiang, China

Abstract

Two phosphate-and potassium-solubilizing strains (KNP413 and KNP414) were isolated from the soil of Tianmu Mountain, Zhejiang Province (China) and they were phenotypically and phylogenetically characterized. Both isolates effectively dissolved mineral phosphate and potassium, while strain KNP414 showed higher dissolution capacity even than Bacillus mucilaginosus AS1.153, the inoculant of potassium fertilizer widely used in China. When grown on Aleksandrov medium, both strains were rod-shaped spore-formers with a large capsule, and they formed slimy and translucent colonies. The DNA G+C contents were 57.7 mol% for strain KNP413 and 56.1 mol% for strain KNP414. Strain KNP413 shared a 16S rRNA gene sequence similarity of more than 99.1% with strain KNP414 and Bacillus mucilaginosus strains HSCC 1605 and YNUC0001, and a 94.6% similarity with Bacillus mucilaginosus VKM B-1480D, the type strain of Bacillus mucilaginosus. Strains KNP413 and KNP414 together with other Bacillus mucilaginosus were clustered with Paenibacillus strains in a group. The use of a specific PCR primer PAEN515F designed for differentiating the genus Paenibacillus from other members of the Bacillaceae showed that strains KNP413 and KNP414 had the same amplified 16S rRNA gene fragment (0.9-kb) as members of the genus Paenibacillus. In conclusion, phosphate-and potassium-solubilizing strains KNP413 and KNP414 should be integrated into the same species different from strain VKM B-1480D and they might be transferred to the genus of Paenibacillus, i.e. Paenibacillus mucilaginosus.

Compost amendment enhances population and composition of phosphate solubilizing bacteria and improves phosphorus availability in granitic regosols

“…The phosphate-solubilizing ability of Paenibacillus spp. has been reported by Hu et al (2006) and Vazquez et al (2000). RP utilization was greater in the PM treatment.…”

“…The addition of organic matter enhances the density of microorganisms (Nishio and Kusano 1980) including phosphate-solubilizing microorganisms, which mobilize the non-labile P by secretion of organic acids such as citric acid, gluconic acid or lactic acid (Chen et al 2006). The increased population density of phosphate-solubilizing microorganisms by organic amendments may be partially responsible for an increased level of P uptake by plants.…”

“…The increased population density of phosphate-solubilizing microorganisms by organic amendments may be partially responsible for an increased level of P uptake by plants. The commonly isolated phosphate solubilizing bacteria (PSB) generally belong to the genera Bacillus or Pseudomonas (Peix et al 2004;Chen et al 2006). In addition, a number of fungal species solubilize P; these species generally belong to the genera Aspergillus or Penicillium (Reyes et al 1999;Vazquez et al 2000).…”

Effect of Potassium and Crop Residue Levels on Potassium Solubilizers and Crop Yield under Maize-Wheat Rotation

“…The potassium solubilising bacterial population in rhizospheric soil was determined by dilution and pour plate method using Aleksandrov medium (Hu et al, 2006). The yield data obtained from two cycles of maize and wheat crops were statistically analysed using the Ftest as per the procedure given by Gomez and Gomez (1984).…”

Isolation and Identification of Two Potassium Solubilizing Fungi from Arid Soil

“…The microorganisms were screened for potassium solubilizing activity on Aleksandrov agar medium (Hu et al, 2006) having: 0.5% glucose, 0.05% magnesium sulfate heptahydrate, 0.0005% iron chloride, 0.01% calcium carbonate, 0.2% calcium phosphate, 0.2% feldspar (potassium aluminum silicate) and 1.8% agar; by spot plate method.…”