Significant progress on upconversionânanoparticle (UCNP)âbased probes is witnessed in recent years. Compared with traditional fluorescent probes (e.g., organic dyes, metal complexes, or inorganic quantum dots), UCNPs have many advantages such as nonâautofluorescence, high chemical stability, large lightâpenetration depth, long lifetime, and less damage to samples. This article focuses on recent achievements in the usage of lanthanideâdoped UCNPs as efficient probes for biodetection since 2014. The mechanisms of upconversion as well as the luminescence resonance energy transfer process is introduced first, followed by a detailed summary on the recent researches of UCNPâbased biodetections including the detection of inorganic ions, gas molecules, reactive oxygen species, and thiols and hydrogen sulfide.