Two-point-separation in super-resolution fluorescence microscope based on up-conversion fluorescence depletion technique

Watanabe, Takeshi; Iketaki, Yoshinori; Omatsu, Takashige; Yamamoto, Kimihisa; Sakai, Makoto; Fujii, Masaaki

doi:10.1364/oe.11.003271

Cited by 80 publications

(49 citation statements)

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“…It has a singularity at the beam axis, where the phase is undefined and the amplitude vanishes. 28) Numerous applications of scalar LG modes, covering several fields in physics, have been found: oam transfer to small particles, 29,30) cold atoms and particles trapping, [31][32][33][34][35] quantum information, 36) high resolution microscopy, 37,38) material processing, 39) etc. A large number of publications exist on the methods of formation of scalar LG modes both inside the cavity (active methods) 16,17,[40][41][42][43] and outside (passive methods), [44][45][46][47] where mode conversion to the required mode was achieved using holograms, spatial light modulators and diffractive optical elements.…”

Section: Introductionmentioning

confidence: 99%

Laguerre-Gaussian modes selection in diode-pumped solid-state lasers

Senatsky

Bisson

et al. 2012

OPT REV

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Over the last twenty years, diode pumping of solid-state lasers has opened new prospects for the mode control and formation of Laguerre-Gaussian (LG) beams, enabling a large variety of applications. Experiments on scalar and vector LG modes selection in Nd 3þ -and Yb 3þ -doped ceramics and crystal lasers carried out at the Institute for Laser Science, Tokyo are reviewed. Selection of LG modes from low to high orders using intra-cavity elements, polarization-selective mirrors, or shaping the pump beam profile, is considered. Illustrations of record-high-order LG hollow modes, multiring modes with highly directional propagation properties, characteristics of cw and pulsed lasers with radially or azimuthally polarized beams of high polarization purity are presented. General solutions of the wave equation for the axi-symmetric electric field, which describe LG beams of various profiles, are proposed for data analysis. In parallel, a short review on LG modes selection studies carried out at other laboratories is given.

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Section: Introductionmentioning

confidence: 99%

Laguerre-Gaussian modes selection in diode-pumped solid-state lasers

Senatsky

Bisson

et al. 2012

OPT REV

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“…Such light is referred as an optical vortex [1][2][3][4]. Optical vortices have been widely investigated for applications such as optical trapping and guiding [5][6][7], as well as superresolution microscopy [8,9]. Circularly polarized light has a helical electric field and a spin angular momentum, S@, associated with its circular polarization.…”

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confidence: 99%

Transfer of Light Helicity to Nanostructures

et al. 2013

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We discovered that chiral nanoneedles fabricated by vortex laser ablation can be used to visualize the helicity of an optical vortex. The orbital angular momentum of light determines the chirality of the nanoneedles, since it is transferred from the optical vortex to the metal. Only the spin angular momentum of the optical vortex can reinforce the helical structure of the created chiral nanoneedles. We also found that optical vortices with the same total angular momentum (defined as the sum of the orbital and spin angular momenta) are degenerate, and they generate nanoneedles with the same chirality and spiral frequency. DOI: 10.1103/PhysRevLett.110.143603 PACS numbers: 42.50.Tx, 42.50.Wk, 79.20.Ds, 79.20.Eb Light that has a helical wave front due to an azimuthal phase shift, expðiL Þ (where L is an integer known as the topological charge), carries orbital angular momentum, L@. Such light is referred as an optical vortex [1][2][3][4]. Optical vortices have been widely investigated for applications such as optical trapping and guiding [5][6][7], as well as superresolution microscopy [8,9]. Circularly polarized light has a helical electric field and a spin angular momentum, S@, associated with its circular polarization. Optical vortices with circular polarization exhibit both wave front and polarization helicities, and a total angular momentum, J@ [10-12], which is defined as the sum of the orbital and spin angular momenta. This angular momentum is evidenced by the orbital and spinning motions of trapped particles in optical tweezers.To date, several researchers have intensely studied the interaction of structured light, such as radially polarized beams, with plasmonic or metallic structures [13][14][15]. However, these previous studies mostly focused on optical properties, such as mode selection, plasmon focusing, etc., of plasmonic or metallic structures, including photonic crystals as well as plasmonic waveguides, prepared by conventional integrated photonic circuit techniques based on lithography and chemical etching. There are few reports on the use of structured light itself to form chiral structures on the nanoscale. Recently, we discovered that the helicity of a circularly polarized optical vortex can be directly transferred to an irradiated metal sample, resulting in the formation of chiral nanoneedles [16][17][18]. This is the first demonstration, to the best of our knowledge, of nanostructures created by structured light with angular momenta, and it clearly represents a new scientific phenomenon.We have also investigated control of the chirality of formed nanoneedles by changing the sign of the optical vortex helicity. Chiral nanostructures have the potential to form many new material structures [19], including planar chiral metamaterials [20,21] and plasmonic nanostructures [22,23]. They can also be used to selectively identify the chirality of chemical composites in nanoscale imaging systems, such as atomic force and scanning tunnel microscopes [24][25][26][27].However, it is currently unclear whe...

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“…We will show that after focusing by a high numerical aperture (NA) objective, this vortex beam can generate a small threedimensional dark spot uniformly surrounded by light, which can be used as the erase spot of a two color far-field three-dimensional superresolution microscope [3]. This vortex beam can be considered to be the coherent superposition of two radially (Figure 1(b)) and azimuthally ( Figure 1(c)) polarized beams.…”

Section: Erase Beam and Pump Beammentioning

confidence: 99%

“…Three-dimensional (3D) dark spots surrounded by light (bottle beams) are applied in many areas in optics, such as dark-spot optical traps for atoms [1,2] or as erase beams for super-resolution fluorescence microscopy [3][4][5][6][7]. Several methods have been used to produce 3D bottle beams that have an intensity null surrounded by light in all directions.…”

Section: Introductionmentioning

confidence: 99%

3d Super-Resolution Fluorescence Microscopy Using Cylindrical Vector Beams

Suyama¹,

Zhang²

2013

PIER Letters

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Abstract-We propose a method to obtain nano-scale 3D superresolution in STED fluorescence microscopy. A double-ring-shaped cylindrical vector vortex beam, with an appropriate vortex angle and a proper truncation parameter of the beam, is used to generate a 3D dark spot as the erase spot. A single-ring-shaped radially polarized beam is used as a pump beam, which can generate a sharper 3D bright spot. The volume of the generated 3D dark spot is small and the uniformity of the light wall surrounding the spot is quite high. Consequently, the 3D super-resolution ability of a STED microscope is improved and nano-scale three-dimensional resolutions are obtained.

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Two-point-separation in super-resolution fluorescence microscope based on up-conversion fluorescence depletion technique

Cited by 80 publications

References 0 publications

Laguerre-Gaussian modes selection in diode-pumped solid-state lasers

Laguerre-Gaussian modes selection in diode-pumped solid-state lasers

Transfer of Light Helicity to Nanostructures

3d Super-Resolution Fluorescence Microscopy Using Cylindrical Vector Beams

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