Two populations of immunoglobulin-forming cells in the skate, Raja kenojei: Their distribution and characterization

Tomonaga, Susumu; Kobayashi, Kunihiko; Kajii, Tadashi; Awaya, Kazuhiko

doi:10.1016/0145-305x(84)90063-6

Cited by 33 publications

(14 citation statements)

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“…However, compared to the R. kenojei, the number of I FC in the spleen of the B. aleutica (around 250 to 6001 m m ) was 5 to 10 times larger than that of the R. kenojei (around 50/mm2) [2]. The reason for the large number of IgFC in the spleen of the B. aleutica is not known, but this allowed us to examine statistically embryonal IgFC, since R. kenojei embryos had very few of them in the spleen.…”

Section: Discussionmentioning

confidence: 95%

“…One is a high molecular weight (HMW) Ig of 18S, analogous to mammalian IgM, and the other is a low molecular weight (LMW) Ig of 9 S, belonging to a hitherto undescribed class. In a subsequent report, we have demonstrated the presence of two populations of cells, producing either the HMW or the LMW Ig, that occur in the spleen, the intestinal mucosa, Leidig organ of the esophagus, the epigonal organ and the liver, but not in the thymus [ 2 ] . Among these organs, the spleen contained the highest number of Ig-forming cells (IgFC), the intestinal mucosa an intermediate number and the other organs a much lesser frequency of cells.…”

Section: Introduction 2 Materials and Methodsmentioning

confidence: 95%

“…Preparation of pepsin-digested rabbit IgG (Fab')z and labeling of the (Fab')z with tetramethyl rhodamine isothiocyanate (TRITC), fluorescein isothiocyanate (FITC) or HRP were described previously [2]. …”

Section: Preparation Of Antibodies Labeled With Fluorochromes or Hrpmentioning

confidence: 99%

“…Our previous study on the distributions of IgFC in R. kenojei indicated that the IgFC were predominantly located in the spleen, intermediately in valvular intestinal mucosa and to a lesser extent in the liver, epipigonal organ and Leydig organ of the esophagus in the adult fish [2]. In our preliminary experiments on the search for the IgFC in small embryos of B. aleutica (BA 18 and BA 4 in Table l ) , no cells positive for Ig staining were detected in any organs other than the spleen.…”

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confidence: 99%

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Ontogenic studies on the appearance of two classes of immunoglobulin‐forming cells in the spleen of the Aleutian skate, Bathyraja aleutica, a cartilaginous fish

et al. 1985

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We identified the presence in the Aleutian skate, Bathyraja aleutica, of two classes of immunoglobulins (Ig), a high molecular weight Ig analogous to mammalian IgM and a low molecular weight Ig, similarly to the spiny rasp skate, Raja kenojei, (Kobayashi, K. et al., Mol. Immunol. 1984. 21: 397), using an immunological cross-reaction with the specific antisera to the spiny rasp skate Ig components. The antigenic similarity of the heavy chains of the Ig of the Aleutian skate and those of the spiny rasp skate was less than that between their light chains. Two types of Ig-producing cells, one producing the high molecular weight and the other forming the low molecular weight Ig, were present in the spleen of embryos and adults in the Aleutian skate at a ratio of 5-6:4-5. The number of these Ig-producing cells increased with advancing development of the embryos but was 1/20 to 1/50 of those of adults. Cells, each of which were capable of forming both classes of Ig, were found in the spleen of embryos but not in that of adults. These results suggested that the spleen of the Aleutian skate is the primary lymphoid organ for B lymphocyte differentiation and proliferation, possibly equivalent to the bursa of birds.

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Section: Discussionmentioning

confidence: 95%

Section: Introduction 2 Materials and Methodsmentioning

confidence: 95%

Section: Preparation Of Antibodies Labeled With Fluorochromes or Hrpmentioning

confidence: 99%

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Ontogenic studies on the appearance of two classes of immunoglobulin‐forming cells in the spleen of the Aleutian skate, Bathyraja aleutica, a cartilaginous fish

et al. 1985

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“…and Endo (1984) described the histological structure of the kidneys in Triakis scyllia, Mustelus griseus, and Raja akaei. Microscopic alterations were also described in hepatic tissue of Potamotrygon motoro (Oguri 1985), Scyliorhinus canicula (Genten et al 2009), spleen of Chlamydoselachus anguineus (Tanaka and Goto 1991) and Raja kenojei (Tomonaga et al 1984). …”

Section: Introductionmentioning

confidence: 93%

A histology-based study in apparently healthy wild freshwater stingrays Potamotrygon motoro

et al. 2015

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Histopathological studies are important as a reference in pathological process. There are a few of these studies in elasmobranchs. The aim of this study was to describe the histology of kidney, spleen, liver, and gills of wild apparently healthy Potamotrygon motoro. However, we observed some pathological findings in these animals. Nineteen P. motoro were collected from the Parana River, Brazil. Histology of kidney, spleen, liver, and gills were performed according to usual procedures. Briefly, the organs were immersed in 10 % formaldehyde solution for 48 h and routinely processed, embedded in paraffin (5-6 mm thick), and subsequent staining with hematoxylin-eosin (HE), Perls', and Schmorl's stains. Histological surface areas were measured using Image Pro Plus software 6.3. We observed that melanomacrophages around the portal vein were larger and more abundant than in the area without the bile duct. Moderate steatosis was observed in most fish, large amount of melanomacrophages and lymphocytes in the gills, liver, and spleen comparing with other studies in this species that could suggest that the immune system in these animals is constantly active. No clinical signs were observed in the fish. Studies are necessary to characterize the immune response against pathogens in elasmobranchs, especially in the Potamotrygon species.

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Structure of the lymphoid organs of elasmobranchs

et al. 1996

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Two populations of immunoglobulin-forming cells in the skate, Raja kenojei: Their distribution and characterization

Cited by 33 publications

References 10 publications

Ontogenic studies on the appearance of two classes of immunoglobulin‐forming cells in the spleen of the Aleutian skate, Bathyraja aleutica, a cartilaginous fish

Ontogenic studies on the appearance of two classes of immunoglobulin‐forming cells in the spleen of the Aleutian skate, Bathyraja aleutica, a cartilaginous fish

A histology-based study in apparently healthy wild freshwater stingrays Potamotrygon motoro

Structure of the lymphoid organs of elasmobranchs

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