Two purified oligosaccharide elicitors,N-acetylchitohepatose and tetraglucosyl glucitol, derived fromMagnaporthe grisea cell walls, synergistically activate biosynthesis of phytoalexin in suspension-cultured rice cells

Yamaguchi, Takeshi; Maehara, Yumiko; Kodama, Osamu; Okada, Mitsuo; Matsumura, Masatoshi; Shibuya, Naoto

doi:10.1078/0176-1617-00877

Cited by 23 publications

(10 citation statements)

References 10 publications

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“…This suggests that in rice, and possibly other plants, FACs may function as amplifiers of basal defense responses modulated by the HME fraction-contained elicitor(s). Amplification and/or synergistic action of two elicitors has already been reported in MAMP signaling355152. For example, simultaneous application of fungal elicitor (GlcNAc) 8 and lipopolysaccharide (LPS) enhanced defense responses in rice cells, even at very low concentrations of bacterial LPS.…”

Section: Discussionmentioning

confidence: 99%

Modulation of plant defense responses to herbivores by simultaneous recognition of different herbivore-associated elicitors in rice

Shinya

Hojo

Desaki

et al. 2016

Sci Rep

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Induced plant defense responses against insect herbivores are triggered by wounding and/or perception of herbivore elicitors from their oral secretions (OS) and/or saliva. In this study, we analyzed OS isolated from two rice chewing herbivores, Mythimna loreyi and Parnara guttata. Both types of crude OS had substantial elicitor activity in rice cell system that allowed rapid detection of early and late defense responses, i.e. accumulation of reactive oxygen species (ROS) and defense secondary metabolites, respectively. While the OS from M. loreyi contained large amounts of previously reported insect elicitors, fatty acid-amino acid conjugates (FACs), the elicitor-active P. guttata’s OS contained no detectable FACs. Subsequently, elicitor activity associated with the high molecular mass fraction in OS of both herbivores was identified, and shown to promote ROS and metabolite accumulations in rice cells. Notably, the application of N-linolenoyl-Gln (FAC) alone had only negligible elicitor activity in rice cells; however, the activity of isolated elicitor fraction was substantially promoted by this FAC. Our results reveal that plants integrate various independent signals associated with their insect attackers to modulate their defense responses and reach maximal fitness in nature.

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Section: Discussionmentioning

confidence: 99%

Modulation of plant defense responses to herbivores by simultaneous recognition of different herbivore-associated elicitors in rice

Shinya

Hojo

Desaki

et al. 2016

Sci Rep

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“…Conversely, hexa‐(1,3/1,6)‐β‐glucoside, which has established activity in soybean, did not elicit diterpenoid phytoalexin production in rice. The species‐specific activity of β‐glucans is consistent with the existence of parallel yet separate perception pathways for fungal cell‐wall elicitors (Yamaguchi et al ., , ). To avoid detection, the fungal pathogen Colletotrichum graminicola exhibits reduced β‐1,3‐glucan synthase ( GLS1 ) transcription and glucan production during biotrophic growth in maize (Oliveira‐Garcia and Deising, ).…”

Section: Activation Of Biosynthesis Of Terpenoid Phytoalexins In Monomentioning

confidence: 99%

Biosynthesis, elicitation and roles of monocot terpenoid phytoalexins

et al. 2014

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SUMMARYA long-standing goal in plant research is to optimize the protective function of biochemical agents that impede pest and pathogen attack. Nearly 40 years ago, pathogen-inducible diterpenoid production was described in rice, and these compounds were shown to function as antimicrobial phytoalexins. Using rice and maize as examples, we discuss recent advances in the discovery, biosynthesis, elicitation and functional characterization of monocot terpenoid phytoalexins. The recent expansion of known terpenoid phytoalexins now includes not only the labdane-related diterpenoid superfamily but also casbane-type diterpenoids and b-macrocarpene-derived sequiterpenoids. Biochemical approaches have been used to pair pathway precursors and end products with cognate biosynthetic genes. The number of predicted terpenoid phytoalexins is expanding through advances in cereal genome annotation and terpene synthase characterization that likewise enable discoveries outside the Poaceae. At the cellular level, conclusive evidence now exists for multiple plant receptors of fungal-derived chitin elicitors, phosphorylation of membrane-associated signaling complexes, activation of mitogen-activated protein kinase, involvement of phytohormone signals, and the existence of transcription factors that mediate the expression of phytoalexin biosynthetic genes and subsequent accumulation of pathway end products. Elicited production of terpenoid phytoalexins exhibit additional biological functions, including root exudate-mediated allelopathy and insect antifeedant activity. Such findings have encouraged consideration of additional interactions that blur traditionally discrete phytoalexin classifications. The establishment of mutant collections and increasing ease of genetic transformation assists critical examination of further biological roles. Future research directions include examination of terpenoid phytoalexin precursors and end products as potential signals mediating plant physiological processes.

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“…Methyl jasmonate (MJ) is frequently selected as an exogenous elicitor to induce benzophenanthridine alkaloids (Färber et al 2003). In the elicitor treatment technique, timing of the treatment, as well as the concentration of the elicitor, influence the degree of metabolite production (Lee-Parsons et al 2004) and combined use of some elicitors can have synergic effects (Sudha and Ravishankar 2002;Yamaguchi et al 2002;Yuan et al 2002). An ability to identify synergic regulators and to combine them optimally to exploit their synergic effects could have great value in systems for production of secondary metabolites.…”

Section: Introductionmentioning

confidence: 99%

Enhanced benzophenanthridine alkaloid production and protein expression with combined elicitor in Eschscholtzia californica suspension cultures

et al. 2007

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Production of the benzophenanthridine alkaloids in Eschscholtzia californica suspension cell cultures was optimized by adding 0.5 mg methyl jasmonate (MJ) and 0.02 mg salicylic acid (SA)/g FCW after 7 days cultivation. Sanguinarine reached 24 mg/g DCW by such treatment; 10 times higher than in control cell cultures. MJ and SA induced expression of berberine bridge enzyme and 3'-hydroxy-(S)-N-methylcoclaurine-4'-O-methyltransferase, respectively. MJ plus SA induced over-expression of both enzymes.

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Two purified oligosaccharide elicitors,N-acetylchitohepatose and tetraglucosyl glucitol, derived fromMagnaporthe grisea cell walls, synergistically activate biosynthesis of phytoalexin in suspension-cultured rice cells

Cited by 23 publications

References 10 publications

Modulation of plant defense responses to herbivores by simultaneous recognition of different herbivore-associated elicitors in rice

Modulation of plant defense responses to herbivores by simultaneous recognition of different herbivore-associated elicitors in rice

Biosynthesis, elicitation and roles of monocot terpenoid phytoalexins

Enhanced benzophenanthridine alkaloid production and protein expression with combined elicitor in Eschscholtzia californica suspension cultures

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