Two related recombinases are required for site-specific recombination at dif and cer in E. coli K12

Blakely, Garry W.; May, Gerhard; McCulloch, Richard; Arciszewska, Lidia K.; Burke, Mary Ellen; Lovett, Susan T.; Sherratt, David J.

doi:10.1016/0092-8674(93)80076-q

Cited by 313 publications

(346 citation statements)

References 27 publications

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“…This process is carried out by the combined action of the sitespecific tyrosine recombinases XerC and XerD, which have been extensively characterized in E. coli [Lesterlin et al, 2004]. These enzymes specifically recognize a short sequence, named dif, in the terminal region of the chromosome and introduce an additional crossover, thereby generating two independent DNA molecules [Blakely et al, 1993].…”

Section: The Last Stages Of Dna Replicationmentioning

confidence: 99%

The bacterial nucleoid: A highly organized and dynamic structure

Thanbichler

Wang

Shapiro

2005

J of Cellular Biochemistry

122

101

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Recent advances in bacterial cell biology have revealed unanticipated structural and functional complexity, reminiscent of eukaryotic cells. Particular progress has been made in understanding the structure, replication, and segregation of the bacterial chromosome. It emerged that multiple mechanisms cooperate to establish a dynamic assembly of supercoiled domains, which are stacked in consecutive order to adopt a defined higher-level organization. The position of genetic loci on the chromosome is thereby linearly correlated with their position in the cell. SMC complexes and histone-like proteins continuously remodel the nucleoid to reconcile chromatin compaction with DNA replication and gene regulation. Moreover, active transport processes ensure the efficient segregation of sister chromosomes and the faithful restoration of nucleoid organization while DNA replication and condensation are in progress.

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Section: The Last Stages Of Dna Replicationmentioning

confidence: 99%

The bacterial nucleoid: A highly organized and dynamic structure

Thanbichler

Wang

Shapiro

2005

J of Cellular Biochemistry

122

101

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“…These natural plasmid recombination sites consist of an approximately 30 bp core site, within which strand exchange occurs, and approximately 200 bp of adjacent accessory sequences . Genetic experiments in Escherichia coli have identified two chromosomally encoded related recombinases, XerC and XerD, which bind co-operatively to core recombination sites (Table 1; Colloms et al, 1990;Blakely et al, 1993). In addition to XerC and XerD, recombination at cer requires two accessory proteins, ArgR and PepA (Stirling et al, 1988;1989).…”

Section: Introductionmentioning

confidence: 99%

DNA sequence of recombinase‐binding sites can determine Xer site‐specific recombination outcome

Blake

Ganguly

Sherratt

1997

Molecular Microbiology

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SummaryXer site-specific recombination functions in the stable inheritance of circular plasmids and bacterial chromosomes. Two related recombinases, XerC and XerD, mediate this recombination, which 'undoes' the potential damage of homologous recombination. Xer recombination on natural plasmid sites is preferentially intramolecular, converting plasmid multimers to monomers. In contrast, recombination at the Escherichia coli recombination site, dif, occurs both intermolecularly and intramolecularly, at least when dif is inserted into a multicopy plasmid. Here the DNA sequence features of a family of core recombination sites in which the XerC-and XerD-binding sites, which are separated by 6 bp, were analysed in order to ascertain what determines whether recombination will be preferentially intramolecular, or will occur both within and between molecules. Sequence changes in either the XerC-or XerD-binding site can alter the recombination outcome. Preferential intramolecular recombination between a pair of recombination sites requires additional accessory DNA sequences and accessory recombination proteins and is correlated with reduced affinities of recombinase binding to recombination core sites, reduced XerC-mediated cleavage in vitro, and an apparent increased overall bending in recombinase-core-site complexes.

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“…Bacteria deficient for dif-specific recombination display decreased growth rate and colony forming ability and frequently form filaments with nucleoid partitioning defects (Blakely et al 1991(Blakely et al , 1993Kuempel et al 1991). Recombination between two dif sites is catalyzed by the XerC and XerD recombinases, which are also involved in the resolution of plasmid multimers (Blakely et al 1991(Blakely et al , 1993. It has been proposed that dif facilitates chromosome separation before partition, for instance, by resolving chromosome dimers that result from an odd number of exchanges between sister chromosomes (Blakely et al 1991;Kuempel et al 1991).…”

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Restriction of the activity of the recombination site dif to a small zone of the Escherichia coli chromosome.

Cornet¹,

Patte²,

Louarn³

1996

Genes Dev.

126

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The recombination site dif is the target on the Escherichia coli chromosome of the site-specific recombinases XerC and XerD. The dif/XerC-D system plays a role during the cell cycle, probably by favoring sister chromosome monomerization or separation. A phenomenon of regional control over dif activity, also analyzed in this issue, is demonstrated here by translocation of dif to a series of loci close to the normal locus. We found that the site is physiologically active only within a narrow zone around its natural position.Competence for dif activity does not depend on the sequence of the normal dif activity zone (DAZ), because ~(diD deletions larger than the DAZ result in Dif + bacteria when dif is reinserted at the junction point. Although dif maps where replication normally terminates, termination of replication is not the elicitor. A strain with a large inversion that places dif and its surrounding region close to oriC remains Dif*, even when a Tus-mutation allows replication to terminate far away from it. Preliminary data suggest the possibility that specialized sequences separate the competent zone from the rest of the chromosome. We suspect that these sequences are members of a set of sequences involved in a polarized process of postreplicative reconstruction of the nucleoid structure. We propose that this reconstruction forces catenation links between sister chromosomes to accumulate within the DAZ, where they eventually favor recombination at d/f.[Key Words." Site-specific recombination; dif site domain of activity; E. coli chromosome organization] Received January 10, 1996; revised version accepted March 20, 1996.The

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Two related recombinases are required for site-specific recombination at dif and cer in E. coli K12

Cited by 313 publications

References 27 publications

The bacterial nucleoid: A highly organized and dynamic structure

The bacterial nucleoid: A highly organized and dynamic structure

DNA sequence of recombinase‐binding sites can determine Xer site‐specific recombination outcome

Restriction of the activity of the recombination site dif to a small zone of the Escherichia coli chromosome.

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