Two sampling methods for the Petri dish detection of airborne fungi

Fernández-Rodríguez, Santiago; Molina, Rafael Tormo; Palacios, Inmaculada Silva; Gonzalo-Garijo, Ángela

doi:10.1080/00173134.2011.596218

Cited by 4 publications

(3 citation statements)

References 28 publications

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“… 35 Cultivable method can identify spores at the species level but is time-consuming and depends on the substrate plated and culture condition applied. 36 Cultivable methods generally allow the identification at the species level. Cultivable method is required to perform genotyping and evaluation of antifungal susceptibility of the isolates.…”

Section: Discussionmentioning

confidence: 99%

Monitoring of clinical strains and environmental fungal aerocontamination to prevent invasive aspergillosis infections in hospital during large deconstruction work: a protocol study

et al. 2017

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IntroductionMonitoring fungal aerocontamination is an essential measure to prevent severe invasive aspergillosis (IA) infections in hospitals. One central block among 32 blocks of Edouard Herriot Hospital (EHH) was entirely demolished in 2015, while care activities continued in surrounding blocks. The main objective was to undertake broad environmental monitoring and clinical surveillance of IA cases to document fungal dispersion during major deconstruction work and to assess clinical risk.Methods and analysisA daily environmental survey of fungal loads was conducted in eight wards located near the demolition site. Air was collected inside and outside selected wards by agar impact samplers. Daily spore concentrations were monitored continuously by volumetric samplers at a flow rate of 10 L.min-1. Daily temperature, wind direction and speed as well as relative humidity were recorded by the French meteorological station Meteociel. Aspergillus fumigatus strains stored will be genotyped by multiple-locus, variable-number, tandem-repeat analysis. Antifungal susceptibility will be assessed by E-test strips on Roswell Park Memorial Institute medium supplemented with agar. Ascertaining the adequacy of current environmental monitoring techniques in hospital is of growing importance, considering the rising impact of fungal infections and of curative antifungal costs. The present study could improve the daily management of IA risk during major deconstruction work and generate new data to ameliorate and redefine current guidelines.Ethics and disseminationThis study was approved by the clinical research and ethics committees of EHH.

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Section: Discussionmentioning

confidence: 99%

Monitoring of clinical strains and environmental fungal aerocontamination to prevent invasive aspergillosis infections in hospital during large deconstruction work: a protocol study

et al. 2017

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“…The petri dishes contained APDA plus 0.1% vol/vol Igepal CO-630 (Sigma-Aldrich, Atlanta, GA) and were incubated for 7 to 10 days at 20-22°C, with a 12 h day/night light cycle. Colony counts of B. cinerea were expressed as colony forming units per cubic meter of air (CFU m −3 ) (Fernández Rodríguez et al 2011).…”

Section: Methodsmentioning

confidence: 99%

Postharvest Incidence of Stem End Rot in ‘Hayward’ Kiwifruit Is Related to PreharvestBotrytis cinereaColonization of Floral Parts and Latent Infection

et al. 2020

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Stem end rot (SER) caused by Botrytis cinerea is the primary postharvest disease in the Chilean kiwifruit industry. Relationships between the postharvest occurrence of SER in ‘Hayward’ kiwifruit and the temporal dynamics of earlier B. cinerea colonization of the floral parts (petals, sepals, receptacles, styles) was studied in five orchards over two consecutive seasons in Chile. Weather conditions in the first season favored B. cinerea infection with roughly constant colonization of floral parts up to about 120 days after full bloom, but colonization then increased up until harvest. In the second season, colonization was roughly constant throughout. Latent infections of the fruit occurred in both seasons but were high in the first season and low in the second. Incidence of latent infections at harvest were the best predictors (r > 0.8) of postharvest SER. The number of preharvest infection periods calculated using temperature, leaf wetness, and relative humidity satisfactorily predicted SER incidence by an exponential model, R2 = 0.90, P < 0.001. Results indicated environmental variables play key roles in the temporal dynamics of B. cinerea colonization. Quantification of latent B. cinerea infections in asymptomatic fruit close to harvest, is a practicable way to predict later incidence of SER during storage.

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“…However, fungal spore’s identification only possible at the genus level [ 15 ]. Cultivable methods allow spore identification at the species level but is time-consuming and depends on the substrate plated and culture conditions applied [ 10 ]. Other factors, such as environment and meteorological parameters, may also affect the survivability of fungal spores [ 11 , 14 ].…”

Section: Introductionmentioning

confidence: 99%

Evaluation of Hirst-type spore traps in outdoor Aspergillaceae monitoring during large demolition work in hospital

Loeffert¹,

Vanhems²,

Tissot³

et al. 2018

PLoS ONE

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Demolition can generate fungal spore suspensions in association with various adverse health effects, such as high risk of invasive aspergillosis in immunocompromised patients. One block of Edouard Herriot Hospital was entirely demolished. The aim of the present study was to evaluate Hirst-type spore traps utility in monitoring outdoor Aspergillaceae (Aspergillus spp. + Penicillium spp.) spores in part of Edouard Herriot Hospital (Lyon, France) undergoing major demolition. Three periods were scheduled in 2015: (A) Gutting of building and asbestos removal, (B) Demolition of floors, (C) Excavation and earthwork. Outdoor Aspergillaceae fungal load was monitored by cultivable (Air Ideal®, bioMérieux) and non-cultivable methods (Lanzoni VPPS-2000, Analyzair®, Bologna, Italy). Differences of Aspergillaceae recorded with Hirst-type spore traps were observed between Gerland and Edouard Herriot Hospital. Differences between Aspergillaceae were recorded between day time and night time at Gerland and Edouard Herriot Hospital. Daily paired differences between Aspergillaceae recorded with non-cultivable methodology at Edouard Herriot Hospital and in an area without demolition work were significant in Period A vs Period B (p = 10–4) and Period A vs Period C (p = 10–4). Weak correlation of daily Aspergillaceae recorded by both methods at Edouard Herriot Hospital was significant only for Period C (r = 0.26, p = 0.048, n = 58). Meteorological parameters and type of demolition works were found to heavily influenced Aspergillaceae dispersion. Non-cultivable methodology is a promising tool for outdoor Aspergillaceae scrutiny during major demolition work in hospital, helping infection control staff to rapidly implement control measures.

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Two sampling methods for the Petri dish detection of airborne fungi

Cited by 4 publications

References 28 publications

Monitoring of clinical strains and environmental fungal aerocontamination to prevent invasive aspergillosis infections in hospital during large deconstruction work: a protocol study

Monitoring of clinical strains and environmental fungal aerocontamination to prevent invasive aspergillosis infections in hospital during large deconstruction work: a protocol study

Postharvest Incidence of Stem End Rot in ‘Hayward’ Kiwifruit Is Related to PreharvestBotrytis cinereaColonization of Floral Parts and Latent Infection

Evaluation of Hirst-type spore traps in outdoor Aspergillaceae monitoring during large demolition work in hospital

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