Two Sec13p Homologs, AtSec13A and AtSec13B, Redundantly Contribute to the Formation of COPII Transport Vesicles in<i>Arabidopsis thaliana</i>

Hino, Takeshi; Tanaka, Yuji; Kawamukai, Makoto; Nishimura, Kohji; Mano, Shoji; Nakagawa, Tsuyoshi

doi:10.1271/bbb.110331

Cited by 56 publications

(52 citation statements)

References 30 publications

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“…All primers are shown in Supplemental Table 10. For expression of GFP fusions, coding sequences were transferred by Gateway recombination into pK7FWG2.0 or pK7WGF2.0 (Hellens et al, 2000) or, for expression of nYFP and cYFP fusions, into plasmids pGWnY (AB626693), pnYGW (AB626694), pGWcY (AB626696), and/or pcYGW (AB626696) (Hino et al, 2011).…”

Section: Transient Expression Of Gfp Fusions and Split-yfp Constructsmentioning

confidence: 99%

Functionally Similar WRKY Proteins Regulate Vacuolar Acidification in Petunia and Hair Development in Arabidopsis

Verweij¹,

et al. 2016

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The WD40 proteins ANTHOCYANIN11 (AN11) from petunia (Petunia hybrida) and TRANSPARENT TESTA GLABRA1 (TTG1) from Arabidopsis thaliana and associated basic helix-loop-helix (bHLH) and MYB transcription factors activate a variety of differentiation processes. In petunia petals, AN11 and the bHLH protein AN1 activate, together with the MYB protein AN2, anthocyanin biosynthesis and, together with the MYB protein PH4, distinct genes, such as PH1 and PH5, that acidify the vacuole. To understand how AN1 and AN11 activate anthocyanin biosynthetic and PH genes independently, we isolated PH3. We found that PH3 is a target gene of the AN11-AN1-PH4 complex and encodes a WRKY protein that can bind to AN11 and is required, in a feed-forward loop, together with AN11-AN1-PH4 for transcription of PH5. PH3 is highly similar to TTG2, which regulates hair development, tannin accumulation, and mucilage production in Arabidopsis. Like PH3, TTG2 can bind to petunia AN11 and the Arabidopsis homolog TTG1, complement ph3 in petunia, and reactivate the PH3 target gene PH5. Our findings show that the specificity of WD40-bHLH-MYB complexes is in part determined by interacting proteins, such as PH3 and TTG2, and reveal an unanticipated similarity in the regulatory circuitry that controls petunia vacuolar acidification and Arabidopsis hair development. INTRODUCTIONRegulatory complexes consisting of a MYB, basic helix-loop-helix (bHLH), and WD40 protein (MBW complexes) control multiple pathways involved in the differentiation of epidermal cells in angiosperms (reviewed in Broun, 2005;Koes et al., 2005;Ramsay and Glover, 2005;Ishida et al., 2008). The best understood function of this complex involves the biosynthesis of anthocyanin pigments that color many flowers and fruits. Closely related, and in most cases functionally exchangeable, MYB, bHLH, and WD40 proteins collaboratively activate the transcription of genes that encode enzymes of the anthocyanin pathway in a broad range of species, including distantly related dicots like Arabidopsis thaliana (a Rosid) and petunia (Petunia hybrida; an Asterid) and monocots like maize (Zea mays; Koes et al., 2005;Hichri et al., 2011;Petroni and Tonelli, 2011). In addition, related MBW complexes activate numerous other pathways and processes, such as vacuolar acidification, formation of seed mucilage, and the development of hair cells, a process that seems restricted to smaller sets of species (Koes et al., 2005;Ramsay and Glover, 2005;Serna and Martin, 2006).In petunia flowers, the WD40 protein ANTHOCYANIN11 (AN11) and the bHLH factor AN1 activate structural anthocyanin genes in floral tissues, such as petals and anthers, and a few vegetative tissues (Quattrocchio et al., 1993;de Vetten et al., 1997;Spelt et al., 2000). To this end, AN11 and AN1 interact with paralogous and exchangeable MYB proteins, which are encoded by AN2, AN4, and DEEP PURPLE and are expressed in different tissues (Quattrocchio et al., 1999(Quattrocchio et al., , 2006Albert et al., 2011). Furthermore, AN1 and AN11 activate, in concert...

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Section: Transient Expression Of Gfp Fusions and Split-yfp Constructsmentioning

confidence: 99%

Functionally Similar WRKY Proteins Regulate Vacuolar Acidification in Petunia and Hair Development in Arabidopsis

Verweij¹,

et al. 2016

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“…We used our Gateway vector construction system (Hino et al, 2011;Nakagawa et al, 2008;Nakagawa et al, 2007b) to make destination vectors for BiFC assays. Using these vectors, it is easy to make constructs for detection of protein-protein interactions.…”

Section: Detection Of Protein-protein Interactions In Plant Cells By mentioning

confidence: 99%

“…Using these vectors, it is easy to make constructs for detection of protein-protein interactions. These Gateway vectors have worked well in plant cells (Goto et al, 2011;Hino et al, 2011;Singh et al, 2009). …”

Section: Detection Of Protein-protein Interactions In Plant Cells By mentioning

confidence: 99%

“…Various BiFC vectors have been developed and used in plant research (Bracha-Drori et al, 2004;Diaz et al, 2005;Ding et al, 2006;Goto et al, 2011;Hino et al, 2011;Loyter et al, 2005;Maple et al, 2005;Marrocco et al, 2006;Ohad et al, 2007;Singh et al, 2009;Waadt et al, 2008;Walter et al, 2004;Zamyatnin et al, 2006). All the vectors, including ours, use P 35S to (Hu & Kerppola, 2003;Waadt et al, 2008), the CFP, GFP and YFP in our system were split between residues 174 and 175, and mRFP1, which contains an amino acid substitution of the 66th glutamine to threonine, was split between residues 154 and 155.…”

Section: Destination Vectors For the Multicolor And In Vivo Bifc Assaysmentioning

confidence: 99%

“…Each target protein (gray and black) can be fused at its N-or C-terminus to the N-or C-terminal fragment of the fluorescent protein (light green) express a fusion gene. There are two ways to insert a target gene into the 5' or 3' end of a split fragment of fluorescent protein gene: (1) cloning into a multicloning site using digestion and ligation, and (2) Gateway technology (Hino et al, 2011;Walter et al, 2004). Our BiFC vectors were developed to be compatible with Gateway technology.…”

Section: Destination Vectors For the Multicolor And In Vivo Bifc Assaysmentioning

confidence: 99%

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Two Sec13p Homologs, AtSec13A and AtSec13B, Redundantly Contribute to the Formation of COPII Transport Vesicles inArabidopsis thaliana

Cited by 56 publications

References 30 publications

Functionally Similar WRKY Proteins Regulate Vacuolar Acidification in Petunia and Hair Development in Arabidopsis

Functionally Similar WRKY Proteins Regulate Vacuolar Acidification in Petunia and Hair Development in Arabidopsis

Gateway Vectors for Plant Genetic Engineering: Overview of Plant Vectors, Application for Bimolecular Fluorescence Complementation (BiFC) and Multigene Construction

An Overview of Protein Secretion in Plant Cells

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