Two Selective Enrichment Media for the Isolation of Salmonella From Mechanically Deboned Poultry Meat

Greenwood, D. E.; Swaminathan, B.; Morse, E. V.

doi:10.1111/j.1365-2621.1980.tb06503.x

Cited by 6 publications

(4 citation statements)

References 21 publications

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“…During the ini-tial portion of this study, samples were enriched in tetrathionate with iodine a and 3MC broth, a laboratory-prepared medium that, when compared with tetrathionate and selenite broths, was superior for isolation of salmonellae from poultry meat and that allowed increased growth of S. choleraesuis. 2,4 After the beginning of the study, and in-house trial demonstrated that Rappaport-Vassiliadis broth was superior to 4 other enrichment media, including tetrathionate and 3MC broths, for recovery of S. choleraesuis from experimentally inoculated pig feces (data not shown). For that reason, Rappaport-Vassiliadis R10 broth a was used for enrichment of source 2 samples and approximately 1 third of the source 1 samples.…”

Section: Comparison Of Conventional and Delayed Secondary Enrichment mentioning

confidence: 99%

Comparison of Conventional and Delayed Secondary Enrichment for Isolation of Salmonella Spp. from Swine Samples

et al. 1998

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Section: Comparison Of Conventional and Delayed Secondary Enrichment mentioning

confidence: 99%

Comparison of Conventional and Delayed Secondary Enrichment for Isolation of Salmonella Spp. from Swine Samples

et al. 1998

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“…Samples were enriched by one of three methods: (i) overnight preenrichment in tryptic soy broth (TSB; Difco Laboratories Inc., Detroit, Mich.) at 37°C and overnight enrichment in Rappaport-Vassiliadis 10 broth (RV10; Difco) at 42°C (TSB-RV10 enrichment method), (ii) overnight preenrichment in a laboratory-prepared medium previously designated 3MC broth (2,20) at 37°C and overnight enrichment in RV10 at 42°C (3MC-RV10 enrichment method), and (iii) direct overnight enrichment in RV10 at 42°C (with no secondary enrichment) (RV10 enrichment method). All samples were collected within 100 mi of the laboratory.…”

Section: Methodsmentioning

confidence: 99%

Comparison of Cultivation and PCR-Hybridization for Detection ofSalmonellain Porcine Fecal and Water Samples

Feder

Nietfeld²,

Galland

et al. 2001

J Clin Microbiol

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A total of 150 fecal and water samples from four swine farms were tested for the presence of Salmonella enterica using different enrichment techniques as follows: (i) 92 fecal samples from nursery and farrowing barns at three swine farms were preenriched overnight in tryptic soy broth (TSB) at 37°C followed by overnight enrichment in Rappaport-Vassiliadis 10 broth (RV10) at 42°C; (ii) 24 water samples from the third farm were preenriched overnight in 3MC broth at 37°C followed by overnight enrichment in RV10 at 42°C; and (iii) 34 fecal samples from a fourth farm, a finishing farm, were enriched overnight in RV10 at 42°C with no additional enrichment. Following each of the enrichment techniques, samples were subcultured onto modified semisolid Rappaport-Vassiliadis (MSRV) agar prior to transfer to Hektoen Enteric agar plates for the recovery of viable Salmonella bacteria. Presumptive Salmonella isolates were biochemically and serologically confirmed. For the PCR detection of Salmonella, a 1-ml portion was removed from each sample after the first overnight enrichment and the DNA was extracted using a Sepharose CL-6B spin column. Amplicons (457 bp) derived from primers to the invA and invE genes were confirmed as Salmonella specific on ethidium bromide-stained agarose gels by Southern hybridization with a 20-mer oligonucleotide probe specific for the Salmonella invA gene. Neither the standard microbiological method nor the molecular method detected all of the 65 samples that tested positive by both methods or either method alone. Salmonella bacteria were detected by both cultivation and PCRhybridization in 68% (17 of 25) of the positive samples that were preenriched in TSB, in 73% (11 of 15) of the positive samples preenriched in 3MC broth, and in 24% (6 of 25) of the positive samples enriched in RV10. Agreement between Salmonella detection using cultivation with preenrichment and detection by PCR was 76% using the kappa statistic. However, agreement between Salmonella detection using cultivation without preenrichment and detection by PCR was about 6%; the PCR assay detected 80% (20 of 25) of the 25 positive samples, while Salmonella bacteria were recovered from only 44% (11 of 25) by cultivation. Our results indicate that the PCR-hybridization approach is equivalent to or better than cultivation for detecting Salmonella in swine feces or water samples from swine farms when using the medium combinations evaluated in this study.

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“…Another factor, which may have contributed to the increased efficiency of the MFDI technique, was the use of the mannitol-magnesium Greenwood et al (1980) have demonstrated that MMS broth was significantly better than selenite and tetrathionate broths in the isolation of salmonellae from MDPM. Further, pseudomonads and coliforms, which caused a great deal of interference in the conventional cultural method, did not pose any problems in the MFDI method.…”

Section: Resultsmentioning

confidence: 98%

“…Unless otherwise stated, all bacteriological media and ingredients for media were obtained from Difco Laboratories, Detroit, MI. The mannitol-magnesium chloride-sodium cholate (MMS) medium was prepared according to the procedure described by Greenwood et al (1980). A modified formulation of dulcitol -brilliant green-novobiocin (DBN) semi-solid medium of Swaminathan et al (1978) was used in the MFDI procedure and was prepared as follows: dulcitol 4.0 g, proteose peptone 12.0 g, yeast extract 3.0 g, sodium chloride 5.0 g, sodium thiosulfate 5.0 g, bile salts 15.0 g, ferric ammonium citrate 1.5 g, sodium desoxycholate 2.0 g, and agar 4.0 g were weighed into a 1500 ml Erlenmeyer flask and 1000 ml of distilled water were added.…”

Section: Mechanicallymentioning

confidence: 99%

Rapid Detection of Salmonellae in Mechanically Deboned Poultry Meat

Greenwood

Swaminathan

1981

Poultry Science

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Samples of frozen mechanically deboned poultry (chicken and turkey) meat were examined for the presence of salmonellae by the conventional cultural method and by a modified membrane filter - disc immunoimmobilization (MFD1) procedure. The modified MFD1 procedure consisted of the following steps: pre-enrichment for 18 hr at 35 C; selective enrichment for 6 hr at 35 C; filtration of 1 ml of selective enrichment culture through a .45 micrometer membrane filter; and incubation for 18 to 24 hr at 35 C of the membrane filter on a semi-solid selective medium in the presence of a disc impregnated with Salmonella polyvalent flagellar antiserum. Presence of Salmonella in a sample was indicated by the immobilization of Salmonella on contact with the antiserum in the semi-solid medium. The modified MFDI procedure was found to be more sensitive than the conventional cultural method for the isolation of salmonella from mechanically deboned poultry meat. Of 100 samples analyzed, only 22 were positive for Salmonella by the conventional cultural procedure while 61 were positive by the modified MFDI procedure. The modified MFDI procedure yielded 1 false negative while the conventional procedure yielded 38 false negatives.

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Two Selective Enrichment Media for the Isolation of Salmonella From Mechanically Deboned Poultry Meat

Abstract: Two selective enrichment media were developed for the isolation of salmonellae from foods. The first, experimental broth 1, contained magnesium chloride and sodium cholate as the selective ingredients.

Cited by 6 publications

References 21 publications

Comparison of Conventional and Delayed Secondary Enrichment for Isolation of Salmonella Spp. from Swine Samples

Comparison of Conventional and Delayed Secondary Enrichment for Isolation of Salmonella Spp. from Swine Samples

Comparison of Cultivation and PCR-Hybridization for Detection ofSalmonellain Porcine Fecal and Water Samples

Rapid Detection of Salmonellae in Mechanically Deboned Poultry Meat

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