Two sequence classes of kinetoplastid 5S ribosomal RNA gene revealed among bodonid spliced leader RNA gene arrays

Sant’Ana, Débora de Mello Gonçales; Lukeš, Julius; Sturm, Nancy R.; Campbell, David A.

doi:10.1111/j.1574-6968.2001.tb10890.x

Cited by 15 publications

(5 citation statements)

References 15 publications

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“…The 39-nucleotide exon portion of the SL gene is well conserved at positions 1-10 and 21-39 among all kinetoplastids. In contrast, the AT-rich region at positions 11-20 shows variability (Santana et al 2001). The sequence of the exon positions 11-20 in the bat trypanosomes studied here is identical to that of T. cruzi, T. rangeli, and a variety of related Trypanosoma species (Gibson et al 2000) consistent with their relatedness in the American Trypanosoma clade (Stevens et al 1999 b).…”

Section: Discussionsupporting

confidence: 61%

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A new species of trypanosome, Trypanosoma desterrensis sp. n., isolated from South American bats

2003

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Trypanosomes isolated from South American bats include the human pathogen Trypanosoma cruzi. Other Trypanosoma spp. that have been found exclusively in bats are not well characterized at the DNA sequence level and we have therefore used the SL RNA gene to differentiate and characterize kinetoplastids isolated from bats in South America. A Trypanosoma sp. isolated from hats in southern Brazil was compared with the geographically diverse isolates T. cruzi marinkellei, T. vespertilionis, and T. dionisii. Analysis of the SL RNA gene repeats revealed size and sequence variability among these bat trypanosomes. We have developed hybridization probes to separate these bat isolates and have analysed the DNA sequence data to estimate their relatedness. A new species, Trypanosoma desterrensis sp. n., is proposed, for which a 5S rRNA gene was also found within the SL RNA repeat.

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Section: Discussionsupporting

confidence: 61%

“…(Sturm, Fernandes & Campbell, 1995 ;Dollet, Sturm & Campbell, 2001), Leishmania sp. (Fernandes et al 1994 ;Grisard et al 2000), the suborder Bodonina (Campbell, 1992 ;Santana et al 2001) and the Euglenozoa (Maslov et al 1993). The SL RNA genes are present at 200-300 copies per cell and arranged as tandem repeats.…”

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confidence: 99%

A new species of trypanosome, Trypanosoma desterrensis sp. n., isolated from South American bats

2003

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“…Thirty-four sequenced DNA fragments were SL RNA gene-specific : they contained recognizable SL RNA features including the conserved exon and intron followed by a T-tract and a variable intergenic region. In several cases, the intergenic region of the SL RNA gene repeat contained the 5S rRNA gene, which is linked with SL RNA genes in some trypanosomatids and bodonids (Santana et al 2001). Several cloned and sequenced bands (42B, 44B, 47B, 48B, 50B, 52B, 55B) were dimers, some of which (47B, 48B, 50B, and 52B) differed internally by a single nucleotide in the intergenic region, as seen previously (Dollet et al 2001).…”

Section: R E S U L T S a N D Discussionsupporting

confidence: 58%

Trypanosomatid biodiversity in Costa Rica: genotyping of parasites from Heteroptera using the spliced leader RNA gene

et al. 2004

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The biodiversity of insect trypanosomes is largely unknown, resulting in significant gaps in the understanding of pathogen evolution. A culture-independent preliminary survey of trypanosomatid fauna was conducted for the parasites of Heteroptera (Hemiptera) from several localities in Costa Rica. Trypanosomatid infections were detected by light microscopy of smeared gut contents. Out of 257 insects representing 6 families, infections were found in 62 cases; cultures were obtained for 29 new isolates. Gut material from infected hosts was preserved in the field using an SDS-EDTA buffer solution for subsequent DNA extraction in the laboratory. PCR amplification of the trypanosomatid-specific spliced leader (SL) RNA gene repeats was successful for 60 field samples. Eighteen distinct SL RNA typing units were identified in a set of 28 samples analysed in detail. Cluster analysis indicated that these typing units were unique and thus could represent new species and, in some cases, new genera. This study reveals only a minor fraction of the trypanosomatid biodiversity, which is anticipated to be high.

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“…The candidate SLe-SLi ORF was in the same equivalent position within the genome assembly contigs as the identified dinoflagellate SLe-SLi ORF [9]. Indeed, an equivalent SLe-SLi ORF location has been reported in other species [33][34][35][36]. This process allowed us to identify candidate SLe-SLi ORFs for the SPI agent (figure 2a; electronic supplementary material, figure S4c).…”

Section: Resultsmentioning

confidence: 78%

A diversified and segregated mRNA spliced-leader system in the parasitic Perkinsozoa

et al. 2022

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Spliced-leader trans -splicing (SLTS) has been described in distantly related eukaryotes and acts to mark mRNAs with a short 5′ exon, giving different mRNAs identical 5′ sequence-signatures. The function of these systems is obscure. Perkinsozoa encompasses a diversity of parasitic protists that infect bivalves, toxic-tide dinoflagellates, fish and frog tadpoles. Here, we report considerable sequence variation in the SLTS-system across the Perkinsozoa and find that multiple variant SLTS-systems are encoded in parallel in the ecologically important Perkinsozoa parasite Parvilucifera sinerae . These results demonstrate that the transcriptome of P. sinerae is segregated based on the addition of different spliced-leader (SL) exons. This segregation marks different gene categories, suggesting that SL-segregation relates to functional differentiation of the transcriptome. By contrast, both sets of gene categories are present in the single SL-transcript type sampled from Maranthos, implying that the SL-segregation of the Parvilucifera transcriptome is a recent evolutionary innovation . Furthermore, we show that the SLTS-system marks a subsection of the transcriptome with increased mRNA abundance and includes genes that encode the spliceosome system necessary for SLTS-function. Collectively, these data provide a picture of how the SLTS-systems can vary within a major evolutionary group and identify how additional transcriptional-complexity can be achieved through SL-segregation.

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Two sequence classes of kinetoplastid 5S ribosomal RNA gene revealed among bodonid spliced leader RNA gene arrays

Cited by 15 publications

References 15 publications

A new species of trypanosome, Trypanosoma desterrensis sp. n., isolated from South American bats

A new species of trypanosome, Trypanosoma desterrensis sp. n., isolated from South American bats

Trypanosomatid biodiversity in Costa Rica: genotyping of parasites from Heteroptera using the spliced leader RNA gene

A diversified and segregated mRNA spliced-leader system in the parasitic Perkinsozoa

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