Two shikimate dehydrogenases,<i>VvSDH3</i>and<i>VvSDH4</i>, are involved in gallic acid biosynthesis in grapevine

Bontpart, Thibaut; Marlin, Thérèse; Vialet, Sandrine; Guiraud, Jean-Luc; Pinasseau, Lucie; Meudec, Emmanuelle; Sommerer, Nicolas; Cheynier, Véronique; Terrier, Nancy

doi:10.1093/jxb/erw184

Cited by 67 publications

(81 citation statements)

References 46 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Homologs with highest similarities for ANR and ANS enzymes were selected as single orthologs in the oak genome, because the identity of the following best-matched sequences decreased more than 20%, whereas for LAR and SDH the more closely-related paralog sequence was further analyzed (Table 1). According to a previous classification of SDH isoenzymes (Bontpart et al 2016), SDH1 and SDH2 from Quercus clustered to the groups I and III, respectively (Fig. 2).…”

Section: Tannins Biosynthesis Genesmentioning

confidence: 73%

“…Sequence alignment and phylogenetic tree were performed by Clustal X and NJPlot, respectively. Sequences shown are abbreviated according to Bontpart et al , with species and sequence codes (NCBI or Phytozome 10.1.2 databases) as follows. AtSDH ( Arabidopsis thaliana AAF08579); CasSDH1,2,3 ( Camellia sinensis AIZ93902, AJA40947, AJA40948); CsSDH1,2,3 ( Citrus sinensis orange1.1g010050m, orange1.1g010101m, orange1.1g007151m); DkSDH ( Diospyros kaki BAI40147); EgSDH1,2,3,4,5 (Eucaliptus grandis Eucgr.H01214.1, Eucgr.H04428.1, Eucgr.H04427.1, Eucgr.B01770.2, Eucgr.J00263.6); FvSDH1,2,3,4 ( Fragaria vesca XP_004302480, XP_004302479, XP_004289250 XP_004288087); JrSHD ( Juglans regia AAW65140); NtSDH1,2 ( Nicotiana tabacum AAS90325, AAS90324); Poptr1,2,3,4,5 ( Populus trichocarpa Potri.010G019000, Potri.013G029900, Potri.005G043400, Potri.014G135500, Potri.013G029800); SlSDH1,2,3 ( Solanum lycopersicum AAC17991, XP_010327280, XP_004242317); VvSDH1,2,3,4 ( Vitis vinífera KU163040, KU163041, KU163042, KU163043).…”

Section: Resultsmentioning

confidence: 99%

“…Cell differentiation and environmental signals like light, biotic and abiotic stress are factors that might modulate the expression of late‐genes encoding for CT biosynthesis enzymes, like LAR , ANR and ANS (Peters and Constabel , Miranda et al , Mellway et al ). In contrast, very little is known about the biosynthesis of HT and its regulation, besides the role of SDH activity supplying gallic acid for plant metabolism (Muir et al , Bontpart et al ). In our study, induction of gene expression for tannin biosynthesis enzymes in leaves of Q. ilex has been evidenced at mRNA and enzyme levels 4 days after mechanical defoliation of young plants, a treatment emulating herbivore wounding (Figs and ).…”

Section: Discussionmentioning

confidence: 99%

“…Different metabolic roles have been assigned for the five SDH isoenzymes of Vitis vinifera (Bontpart et al ), with cluster I isoenzyme presenting the highest activity and a pleiotropic role on the different metabolic pathways starting by 3‐dehydroshikimate, whereas the isoenzyme that belongs to cluster III is mainly involved in the biosynthesis of gallic acid and derived HT molecules (Fig. ).…”

Section: Discussionmentioning

confidence: 99%

See 3 more Smart Citations

Regulation by biotic stress of tannins biosynthesis in Quercus ilex: Crosstalk between defoliation and Phytophthora cinnamomi infection

Gallardo

Morcuende

Solla

et al. 2018

Physiologia Plantarum

View full text Add to dashboard Cite

Sustainability of the Mediterranean forest is threatened by oak decline, a disease of holm oak and other Quercus species that is initiated by infection with the oomycete Phytophthora cinnamomi. Focusing on the role of tannins in the chemical defense of plants, this work investigated whether tannins content in Quercus ilex is regulated by biotic stress. Screening of published genomes allowed the identification of Quercus sequences encoding enzymes for early steps of the biosynthesis of phenolic compounds, like hydrolysable tannins and condensed tannins (CT) among others, plus genes involved in the late steps of CT biosynthesis. Four days after treatment of Q. ilex seedlings by mechanical defoliation, P. cinnamomi infection and both stressors simultaneously, mRNA concentrations for tannins biosynthesis enzymes were measured in leaves. Among the transcript amount for shikimate dehydrogenase (SDH, EC 1.1.1.25), anthocyanidin reductase (EC 1.3.1.77), anthocyanidin synthase (EC 1.14.11.19) and leucoanthocyanidine reductase (EC 1.17.1.3), defoliation induced gene expression for SDH2 isoenzyme. About 4 days after infection of roots by P. cinnamomi, this up‐regulation was canceled and SDH enzyme activity decreased. Furthermore, during this late stage of biotrophic interaction the pathogen switched off the correlation engaged by defoliation between the expression of SDH1 and SDH2 encoding genes and chemical defenses corresponding to total tannins, which were down‐regulated. Thus, tannins biosynthesis in seedlings of Q. ilex is induced after mechanical defoliation whereas infection by the pathogen interferes with this regulation, potentially increasing the susceptibility of plants to herbivory and aggravating the impact of biotic stress.

show abstract

Section: Tannins Biosynthesis Genesmentioning

confidence: 73%

Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

See 2 more Smart Citations

Regulation by biotic stress of tannins biosynthesis in Quercus ilex: Crosstalk between defoliation and Phytophthora cinnamomi infection

Gallardo

Morcuende

Solla

et al. 2018

Physiologia Plantarum

View full text Add to dashboard Cite

show abstract

“…11,12 However, gallic acid often is a byproduct in the microbial production of DHS or shikimate. 11,12 However, gallic acid often is a byproduct in the microbial production of DHS or shikimate.…”

Section: Production Of Dhs Derivativesmentioning

confidence: 99%

Expanding the repertoire of aromatic chemicals by microbial production

Song

Chen

et al. 2018

J of Chemical Tech & Biotech

View full text Add to dashboard Cite

Microbial production of aromatic chemicals would greatly contribute to solving the problems with fossil resource supply and environmentally sustainable development. Engineering and extending the shikimate/aromatic amino acid biosynthetic pathways are important routes for microbial production of various aromatic chemicals. With advances in metabolic engineering and synthetic biology, we can broaden the product spectrum and obtain several valuable and novel aromatic chemicals from renewable feedstocks. Here, in this review, the latest research progress on microbial production of various aromatic chemicals, and recent metabolic engineering and synthetic biology strategies targeting the central carbon metabolism, the shikimate and aromatic amino acid biosynthetic pathways are summarized and discussed. This work aims to provide some valuable tips for the construction of cost‐effective engineered strains for producing various aromatic chemicals. © 2018 Society of Chemical Industry

show abstract

Phenylpropanoid Metabolism

Rock

2017

Encyclopedia of Life Sciences

View full text Add to dashboard Cite

Phenylpropanoid compounds encompass a wide range of structural classes with diverse biological functions in defence, survival and structural support associated with normal plant development (belying the term 'secondary metabolite'). The biosynthesis of phenylpropanoids is regulated by diverse environmental stimuli. Phenylpropanoid metabolism (other than flavonoids, not covered here) occupies a central place in the general aromatic metabolism of plants from shikimate to phenylalanine to lignin polymers and also to coumarins, phenolic volatiles and hydrolysable tannins. In recent years, genetics and biochemistry, along with methodology‐driven, computational, transgenic and comparative transcriptomic approaches, have led to significant advances in the identification of the families of genes encoding enzymes, transporters, regulatory factors involved in phenylpropanoid metabolism and a clearer picture of their functions in biotic and abiotic stress responses, plant development and enzyme/pathway evolution driven by interactions of species with their environment. Key Concepts Having one phenyl aromatic ring with one or more hydroxyl groups attached gives phenylpropanoids amphiphilic and reducing properties, underlying their ability to physically interact with other biomolecules. Plants invest a large percentage of their fixed carbon into synthesising phenylpropanoids, from simple volatile phenolic acids such as the defence hormone salicylic acid to complex polyphenolic flavonoids encompassing thousands of compounds with myriad physiological and adaptive functions. The shikimate pathway is the starting point for phenylpropanoid biosynthesis from shikimate product phenylalanine ( l ‐Phe), via the intermediate chorismate, which also serves as substrate for the synthesis of quinones and tocopherols important as electron acceptors in photosynthesis and aerobic respiration. Phenylpropanoid biosynthesis proceeds from deamination of Phe to cinnamate by the rate‐limiting and environmentally regulated enzyme PAL, followed by oxidation of the ring to p ‐coumarate, its activation with coenzymeA and a series of hydroxylation, methylation and reduction reactions to give cinnamic acids, ‐aldehydes and ‐alcohols that serve as substrates to generate a wide range of complex structures, notably polymers of coumaroyl, conferyl and sinapyl alcohols comprising lignin. Recent studies have established the major pathway in plants which proceeds from p ‐coumaroyl‐CoA → p ‐coumaryl‐shikimate → caffeoyl‐shikimate → caffeoyl‐CoA → feruloyl CoA → coniferaldehyde → 5‐OH coniferaldehyde → sinapaldehyde → sinapate/sinapyl alcohol (oxidation versus reduction, respectively), instead of the original model of a grid/matrix of parallel ring oxidation and side‐chain redox pathways from hydroxycinnamic acids to alcohols. Synthesis involves three subcellular compartments: chloroplast for Phe, cytosol for sinapoyl‐esters and the vacuole for trans‐esterifications. The identity of specific transporters, temporal‐ or organ‐specific regulatory factors for phenylpropanoid flux to (in)soluble polymers in the apoplast in response to biotic and abiotic stressors and whether lignin formation proceeds via precise channeling of individual precursors through metabolons (temporary structural–functional complexes formed between sequential enzymes) are key questions of practical significance that remain to be answered.

show abstract

Two shikimate dehydrogenases,VvSDH3andVvSDH4, are involved in gallic acid biosynthesis in grapevine

Abstract: HighlightFunctional analysis of the four grapevine shikimate dehydrogenases (VvSDH1–4) reveals that two of them are involved in gallic acid biosynthesis.

Cited by 67 publications

References 46 publications

Regulation by biotic stress of tannins biosynthesis in Quercus ilex: Crosstalk between defoliation and Phytophthora cinnamomi infection

Regulation by biotic stress of tannins biosynthesis in Quercus ilex: Crosstalk between defoliation and Phytophthora cinnamomi infection

Expanding the repertoire of aromatic chemicals by microbial production

Phenylpropanoid Metabolism

Contact Info

Product

Resources

About