Two single nucleotide polymorphisms sites in α1-AT gene and their association with somatic cell score in Chinese Holstein cows

Abstract

BackgroundAlpha 1-antitrypsin (α1-AT) may affect the susceptibility of mastitis in dairy cattle for its possible role in the protection of lactoferrin from proteolytic degradation in the mammary. Milk somatic cell score (SCS) is a logarithmic transformation of the milk somatic cell count widely used as an index to evaluate mastitis. To study the relationships of α1-AT gene and SCS in Chinese Holstein cows, methods of PCR-SSCP, DNA sequencing, PCR-RFLP, and CRS-PCR technologies were used to detect single nucleotide polymorphisms sites in α1-AT gene.ResultsTwo polymorphic sites at G5503A and G5746C of α1-AT gene were found. AA (0.3633), AB (0.4644) and BB (0.1723) genotypes were detected at G5503A site, CC (0.3483), CD (0.4906) and DD (0.1611) genotypes were found at G5746C in Chinese Holstein cows. Least squares mean of SCS for individuals with BB genotype was significantly lower than that with AA and AB genotype (p < 0.01), and that with AB genotype was significantly lower than that with AA (p < 0.05). There was no significant difference among individuals with CC, CD and DD genotypes (p > 0.05). Least squares mean of SCS for individuals with BBDD genotype combination were significantly lower than those with AACC and AACD (p < 0.05).ConclusionsStatistical analysis indicated that B allele and BBDD genotype combination of α1-AT can improve mastitis resistance in dairy cattle.

Effects of FecB Mutation on Estrus, Ovulation, and Endocrine Characteristics in Small Tail Han Sheep

“…P ≤ 0.01 was considered extremely significant. The statistical analyses of genotype frequency, allele frequency, and polymorphism information content were performed with reference to the formulas reported by Guo et al ( 28 ). Traits in estrus determination, ovulation time, hormone concentrations in serum, and gene expression were continuous data.…”

Identification of Milk Fat Metabolism-Related Pathways of the Bovine Mammary Gland during Mid and Late Lactation and Functional Verification of the ACSL4 Gene

“…Milk composition was detected using mid-infrared spectrometry (MilkoScan Minor, Foss Analytics, Hillerød, Denmark) [ 17 ]. Somatic cell counts (SCCs) in milk samples were estimated by MilkoScan (FOSS 6000, Denmark) and somatic cell scores (SCSs) was calculated according to the formula: SCS = (log 2 SCC/10,000) + 3 [ 18 ]. The daily milk yield of the dairy cows was recorded at 180 d, 210 d, 240 d, and 270 d. Surgical methods collected dairy cow mammary glands at mid (180 d) and late (270 d) lactation [ 19 , 20 ].…”