Two-stage cultivation of the marine microalga Chlorella salina for starch and carbohydrate production

Chong, Jia Fong; Fadhullah, Widad; Lim, Vuanghao; Lee, Chee Keong

doi:10.1007/s10499-019-00385-3

Cited by 14 publications

(4 citation statements)

References 53 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…SHH (described in point 2.1) was the only carbon source used in these batch assays. The maximum accumulation capacity of compounds was assayed without the addition of nitrogen, since it has been reported that restricting this nutrient in the culture could lead to the accumulation of storage compounds by the cell ( Guerfali et al, 2018 ; Chong et al, 2019 ). Six assays, in triplicate, were carried out to evaluate the effect of increasing SHH doses (30, 75, and 120 Cmmol/L) on the production of the compounds of interest ( Table 1 ).…”

Section: Methodsmentioning

confidence: 99%

Toward the use of mixed microbial cultures for the biological production of adipic and levulinic acid

et al. 2023

View full text Add to dashboard Cite

Biological synthesis of high added-value compounds like adipic acid (AA), levulinic acid (LA), or polyhydroxybutyrate (PHB) using pure culture has been separately reported. However, pure culture requires sterile conditions and the use of specific carbon sources resulting in high operating costs. Different alternatives based on the use of mixed microbial cultures (MMC) have been explored to resolve this problem. MMC have been widely reported for the production of PHB, but scarcely reported for LA production and never for AA synthesis. This work presents a novel strategy for the co-production of AA LA, and PHB using MMC. The strategy consists in selecting an MMC producer of AA, LA and PHB from an inoculum obtained from a wastewater treatment plant, which is then subjected to the feast and famine culture strategy in a sequential batch reactor, coupled with a batch reactor step to enhance the accumulation of AA and LA. The results showed that the MMC could produce a 16 ± 2, 23 ± 1 and 5 ± %1 (g compound/g volatile solids) of AA, LA and PHB, respectively, using a non-fermented residual biomass rich in pentose, namely synthetic hemicellulose hydrolysate (SHH) as the carbon source. These results contribute to generating future research to better understand and optimise the biosynthesis of these compounds by MMC.

show abstract

Section: Methodsmentioning

confidence: 99%

Toward the use of mixed microbial cultures for the biological production of adipic and levulinic acid

et al. 2023

View full text Add to dashboard Cite

show abstract

“…The findings shows that a 12 h: 12 h light-dark cycle, 5% v/v CO2 concentration, and a combination of nitrogen and phosphorous limits is the ideal culture condition with highest starch and carbohydrate content. However, as compared to culture in synthetic medium, wastewater aerated with 5% CO2 is considered more sustainable, with double metabolite accumulation [25]. According to Das et al [26] the leftover algal biomass after extracting lipids can also be used in bioplastic production and the result shows 27% PHB content.…”

Section: Algae Used In Bioplastic Productionmentioning

confidence: 99%

Algae Based Bio-Plastics: Future of Green Economy

Sreenikethanam¹,

Bajhaiya²

2022

Biorefineries - Selected Processes

View full text Add to dashboard Cite

Plastic has become one of the most crucial requirements of the modern-day living. The continuous reliance on the petroleum-based, non-biodegradable plastics has resulted in increased global environmental damage and rapid depletion of fossil fuels. Bioplastic, with remarkably similar properties to petroleum-based plastics is a promising alternative to overcome these emerging challenges. Despite the fact that algae and cyanobacteria are feasible alternative source for bio-plastic, there have been limited studies on strain selection and optimization of culture conditions for the bio plastic production. Naturally, algae and cynobacteria can accumulate higher amount of metabolites under stress conditions however one of the recent study on genetic engineering of Synechocystis sp. coupled with abiotic stresses showed up to 81% of increase in PHB level in the transformed lines. This chapter provides summary of various studies done in the field of algal bio-plastics, including bioplastic properties, genetic engineering, current regulatory framework and future prospects of bioplastic. Further the applications of bioplastics in industrial sector as well as opportunities and role of bio plastic in green economy are also discussed.

show abstract

“…In addition, the nutrient factor-integrated strategy can be used to improve the accumulation of metabolites. A two-stage strategy with a combination of nitrogen and sulfur limitation significantly enhanced starch and carbohydrate production in Chlorella salina [112].…”

Section: Multiple Factors Integrated Cultivation Strategiesmentioning

confidence: 99%

Comprehensive Utilization of Marine Microalgae for Enhanced Co-Production of Multiple Compounds

Wang

Chua

et al. 2020

Marine Drugs

View full text Add to dashboard Cite

Marine microalgae are regarded as potential feedstock because of their multiple valuable compounds, including lipids, pigments, carbohydrates, and proteins. Some of these compounds exhibit attractive bioactivities, such as carotenoids, ω-3 polyunsaturated fatty acids, polysaccharides, and peptides. However, the production cost of bioactive compounds is quite high, due to the low contents in marine microalgae. Comprehensive utilization of marine microalgae for multiple compounds production instead of the sole product can be an efficient way to increase the economic feasibility of bioactive compounds production and improve the production efficiency. This paper discusses the metabolic network of marine microalgal compounds, and indicates their interaction in biosynthesis pathways. Furthermore, potential applications of co-production of multiple compounds under various cultivation conditions by shifting metabolic flux are discussed, and cultivation strategies based on environmental and/or nutrient conditions are proposed to improve the co-production. Moreover, biorefinery techniques for the integral use of microalgal biomass are summarized. These techniques include the co-extraction of multiple bioactive compounds from marine microalgae by conventional methods, super/subcritical fluids, and ionic liquids, as well as direct utilization and biochemical or thermochemical conversion of microalgal residues. Overall, this review sheds light on the potential of the comprehensive utilization of marine microalgae for improving bioeconomy in practical industrial application.

show abstract

Two-stage cultivation of the marine microalga Chlorella salina for starch and carbohydrate production

Cited by 14 publications

References 53 publications

Toward the use of mixed microbial cultures for the biological production of adipic and levulinic acid

Toward the use of mixed microbial cultures for the biological production of adipic and levulinic acid

Algae Based Bio-Plastics: Future of Green Economy

Comprehensive Utilization of Marine Microalgae for Enhanced Co-Production of Multiple Compounds

Contact Info

Product

Resources

About