Two-step degradation of pyrene by white-rot fungi and soil microorganisms

Wiesche, Carsten in der; Martens, Rainer; Zadražil, František

doi:10.1007/s002530050876

Cited by 67 publications

(26 citation statements)

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“…The lack of inoculated fungal growth in these non-autoclaved columns could be explained by the inability of the fungus to compete with the indigenous soil microorganisms (Radtke et al, 1994;in der Wiesche et al, 1996;Martens and Zadrazil, 1998;Andersson et al, 2000). Surprisingly, the amount of fungal carbon was even higher in the control-medium columns compared to the Schizophyllum columns.…”

Section: Discussionmentioning

confidence: 86%

Variable effects of labile carbon on the carbon use of different microbial groups in black slate degradation

Seifert¹,

Trumbore²,

Xu³

et al. 2011

Geochimica et Cosmochimica Acta

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Weathering of ancient organic matter contributes significantly to biogeochemical carbon cycles over geological times. The principle role of microorganisms in this process is well recognized. However, information is lacking on the contribution of individual groups of microorganisms and on the effect of labile carbon sources to the degradation process. Therefore, we investigated the contribution of fungi, Gram-positive and Gram-negative bacteria in the degradation process using a column experiment. Investigations were performed on low metamorphic black slates. All columns contained freshly crushed, sieved (0.63-2 mm), not autoclaved black slates. Two columns were inoculated with the lignite-degrading fungus Schizophyllum commune and received a culture medium containing 13 C labeled glucose, two columns received only this culture medium and two control columns received only water.The total mass balance was calculated from all carbon added to the slate and the CO 2 and DOC losses. Phospholipid fatty acids (PLFA) were extracted to investigate microbial communities. We used both the compound specific 14 C and 13 C signal of the PLFA to quantify carbon uptake from black slates and the glucose of the culture medium, respectively.The total carbon loss in these columns exceeded the amount of added carbon by approximately 60%, indicating that black slate carbon has been used. PLFA associated with Gram-positive bacteria dominated the indigenous community and took up 22% of carbon from black slate carbon, whereas PLFA of Gram-negative bacteria used only 8% of carbon from the slates. PLFA of Gram-negative bacteria and fungi were both mostly activated by the glucose addition. The added Schizophyllum did not establish well in the columns and was overgrown by the indigenous microbial community. Our results suggest that especially Gram-positive bacteria are able to live on and degrade black slate material. They also benefit from easy degradable carbon from the nutrient broth. In natural environments priming due to root exudates might consequently enhance weathering.

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Section: Discussionmentioning

confidence: 86%

Variable effects of labile carbon on the carbon use of different microbial groups in black slate degradation

Seifert¹,

Trumbore²,

Xu³

et al. 2011

Geochimica et Cosmochimica Acta

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“…Although the fungal rates of degradation of PAHs are slow and ine⁄cient compared to those of bacteria, fungi have the ability to hydroxylate a wide variety of PAHs; therefore their ecological role could be signi¢cant. Furthermore, these polar intermediates can be mineralized by soil bacteria or detoxi¢ed to innocuous compounds [28]. In addition, fungi have an advantage over bacteria since fungal mycelium could grow into the soil and distribute itself through the solid matrix to degrade the PAHs.…”

Section: Discussionmentioning

confidence: 99%

Degradation of pyrene by indigenous fungi from a former gasworks site

Saraswathy

2002

FEMS Microbiology Letters

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Indigenous fungi isolated from soil of a former gasworks site were investigated in submerged cultures with pyrene as the sole carbon source. Five fungal strains capable of degrading pyrene included one strain of Trichoderma harzianum and four strains with characteristics of the genus Penicillium. These are identified as Penicillium simplicissimum, Penicillium janthinellum, Penicillium funiculosum and Penicillium terrestre. A maximum of 75% of 50 mg l 31 and 67% of 100 mg l 31 of pyrene was removed by the fast degrading strain P. terrestre at 22 ‡C during 28 days of incubation. The slower degrader P. janthinellum was able to remove 57% of 50 mg l 31 and about 31.5% of 100 mg l 31 pyrene. Degradation of pyrene is directly correlated with biomass development. To the best of our knowledge, this is the first time that fungi have been reported to use pyrene as the sole carbon and energy source. They may be ideal candidates for effective bioremediation of polycyclic aromatic hydrocarbons. ß

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“…has been studied in liquid culture (8,9,10), in a straw substrate (29,36,64), and in sterile soil (48). Degradation of more than 25% of the initial amount of PAHs containing four to six aromatic rings was found in straw with an initial PAH concentration of 10 ppm.…”

Section: Discussionmentioning

confidence: 99%

“…It has to be noted that Pleurotus spp. are strongly competitive fungal species which can readily colonize nonsterile soil (29,37) and even decrease the population of soil bacteria (28). The disappearance of less-soluble compounds with five or six aromatic rings (benzo-[a]fluoranthene, benzo[k]fluoranthene, benzo[a]pyrene, dibenzo[a,h]anthracene, and benzo[ghi]perylene) was particularly enhanced in the presence of P. ostreatus.…”

Section: Discussionmentioning

confidence: 99%

Influence of Cadmium and Mercury on Activities of Ligninolytic Enzymes and Degradation of Polycyclic Aromatic Hydrocarbons by Pleurotus ostreatus in Soil

Baldrián

Wiesche²,

Gabriel

et al. 2000

Appl Environ Microbiol

199

110

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The white-rot fungus Pleurotus ostreatus was able to degrade the polycyclic aromatic hydrocarbons ( anthracene (41%), the fungus enhanced the disappearance of less-soluble polycyclic aromatic compounds containing five or six aromatic rings. Although the heavy metals in the soil affected the activity of ligninolytic enzymes produced by the fungus (laccase and Mn-dependent peroxidase), no decrease in PAH degradation was found in soil containing Cd or Hg at 10 to 100 ppm. In the presence of cadmium at 500 ppm in soil, degradation of PAHs by soil microflora was not affected whereas the contribution of fungus was negligible, probably due to the absence of Mn-dependent peroxidase activity. In the presence of Hg at 50 to 100 ppm or Cd at 100 to 500 ppm, the extent of soil colonization by the fungus was limited.

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Two-step degradation of pyrene by white-rot fungi and soil microorganisms

Cited by 67 publications

References 0 publications

Variable effects of labile carbon on the carbon use of different microbial groups in black slate degradation

Variable effects of labile carbon on the carbon use of different microbial groups in black slate degradation

Degradation of pyrene by indigenous fungi from a former gasworks site

Influence of Cadmium and Mercury on Activities of Ligninolytic Enzymes and Degradation of Polycyclic Aromatic Hydrocarbons by Pleurotus ostreatus in Soil

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