Two‐step modification of silica for efficient adsorption of phosphatidylcholine in water and application in phospholipase D‐catalyzed transphosphatidylation

Wang, Jiao; Yu, Wenyu; Zhang, Xiaoli; Zhang, Tiantian; Wang, Huan; Shu, Wenfang; Liu, Li; Zhao, Binxia; Li, Binglin

doi:10.1002/btpr.2949

Cited by 6 publications

(3 citation statements)

References 29 publications

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“…In addition, a large number of PA byproducts were generated during the transformation process . In previous studies, the hydrolysis reaction rates of PLD could be reduced by changing the reaction system , or using immobilized enzymes, − which is difficult to scale up. Herein, we employed protein engineering to enhance the transphosphatidylation efficiency by increasing the space and hydrophobicity of PLD as well as manipulating the coordination of l -Ser within the active site.…”

Section: Discussionsupporting

confidence: 84%

Rational Design of Phospholipase D to Improve the Transphosphatidylation Activity for Phosphatidylserine Synthesis

Liu²,

Song

et al. 2022

J. Agric. Food Chem.

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Phosphatidylserine (PS) has been widely used in the fields of food and medicine, among others, owing to its unique chemical structure and health benefits. However, the phospholipase D (PLD)-mediated enzymatic production of PS remains a challenge due to the low transphosphatidylation activity of PLD. Therefore, in the present study, we designed a maltose-binding protein (MBP) tag and a PLD co-expression method to achieve the expression of soluble PLD in Escherichia coli. A “reconstruct substrate pocket” strategy was then proposed based on the catalytic mechanism and molecular dynamics simulation, expanding the substrate pocket and manipulating the coordination of l-Ser within the active site. The best mutant (Sr MBPPLDMu6) exhibited a 2.04-fold higher transphosphatidylation/hydrolysis ratio than the wild-type Furthermore, under optimal conditions, Mu6 produced 58.6 g/L PS with 77.2% conversion, within 12 h on a 3 L scale, which demonstrates the potential of the proposed method for industrial application.

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Section: Discussionsupporting

confidence: 84%

Rational Design of Phospholipase D to Improve the Transphosphatidylation Activity for Phosphatidylserine Synthesis

Liu²,

Song

et al. 2022

J. Agric. Food Chem.

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“…Thus, the strong repulsion among positive charges might not be beneficial for the adsorption of PC. Our previous studies also supported this point that the amphipathic PC molecule would like to adsorb on the suitable support surface, which is neither extremely hydrophilic nor hydrophobic 31 . Another important reason might be the aggregation of hydrophilic carriers in the hydrophobic organic solution.…”

Section: Resultssupporting

confidence: 55%

“…Our previous studies also supported this point that the amphipathic PC molecule would like to adsorb on the suitable support surface, which is neither extremely hydrophilic nor hydrophobic. 31 Another important reason might be the aggregation of hydrophilic carriers in the hydrophobic organic solution. This phenomenon was enlarged when MOP was used for immobilization.…”

Section: Pla1 Immobilized On Different Carriersmentioning

confidence: 99%

Efficient immobilized phospholipase A1 on Mo‐basing nanomaterials for enzymatic degumming

Wang

Guo

et al. 2022

Biotechnology Progress

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Six kinds of Mo-basing nanomaterials (MoO 3 , MoO 3 @Ru, Mo-PDA, MoP C , MoP, CNT@MoS 2 ) were successfully synthesized, which were employed as carriers to immobilize phospholipase A 1 (PLA1) for the hydrolysis of phospholipids (PLs).PLA1 was immobilized by a simple adsorption-precipitation-cross-linking to form an "enzyme net" covering on nanoparticles. The greatest advantage of these nanoparticles was their strong hydrophilic surface. It not only permitted their dispersion in the aqueous phase, but also showed the strong affinity for PLs in the organic phase, because amphiphilic PLs had the polar head group and higher hydrophilicity than other oils components. Michaelis-Menten analysis revealed that higher catalytic activity and enzymeÀsubstrate affinity were observed in several immobilized PLA1 than its free form. MoO 3 was confirmed to be the best candidate for carrier. The highest specific activity of MoO 3 -immobilized PLA1 reached 43.1 U/mg, which was about 1.8 times higher than that of free PLA1 (24.4 U/mg). In addition, the stability and recycling were also enhanced. The robust immobilized PLA1 was prepared in this work, showing great potential for the enzymatic degumming.

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3‐Octanoylthio‐1‐propyltriethoxysilane functionalized silica/rubber composites for application in tire: Structure, performance and synergism

et al. 2022

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Increasing the polymer‐filler interaction is a challenge to develop high performance rubber composites. In this study, a new blocked mercaptosilane (3‐Octanoylthio‐1‐propyltriethoxysilane) was used to increase the rubber‐silica interaction by modifying highly dispersible silica and fumed silica surface with this silane. A two‐step process was used for the modification. Successful hydrolysis and condensation reaction was proved by Fourier‐transform infrared spectroscopy. Grafting density and grafting percentage were determined using thermogravimetric analysis. The modified silica/rubber composites were compared with the compositions where silane was separately added during mixing. Dynamic modulus versus strain signatures demonstrated strong polymer‐filler interaction after functionalization of the silica surface. The composites containing modified silica demonstrated improved mechanical properties and abrasion resistance over the unmodified one, which were correlated with the dispersion index. Dynamic mechanical analysis showed 28% improvement of rolling resistance and 67% improvement of ice traction after modification. Amount of silane modified silica also varied in total filler loading for precipitated silica/rubber composites. Silica‐rubber composites, where hybrid silica structure (75/25 modified and pristine silica ratio) was used, displayed interesting property to fulfill the requirement of green tire composites. Such a finding can be extended to developing tires for energy efficient vehicles.

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Two‐step modification of silica for efficient adsorption of phosphatidylcholine in water and application in phospholipase D‐catalyzed transphosphatidylation

Cited by 6 publications

References 29 publications

Rational Design of Phospholipase D to Improve the Transphosphatidylation Activity for Phosphatidylserine Synthesis

Rational Design of Phospholipase D to Improve the Transphosphatidylation Activity for Phosphatidylserine Synthesis

Efficient immobilized phospholipase A1 on Mo‐basing nanomaterials for enzymatic degumming

3‐Octanoylthio‐1‐propyltriethoxysilane functionalized silica/rubber composites for application in tire: Structure, performance and synergism

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