Two-step sequential reaction catalyzed by layer-by-layer assembled urease and arginase multilayers

Three‐Dimensional Interdigitated Electrode Array as a Tool for Surface Reactions Registration

“…The layer-by-layer (LbL) self-assembly of polyelectrolytes is one of the simplest ways to immobilise enzymes and was successfully used to immobilise urease on inorganic or polymer solid substrates [13][14][15] alternating polycation and urease layers. The presence of polyelectrolyte layers at the solid/liquid interface significantly affects the surface conductivity [16,17] which can be easily registered with the 3D-IDEA sensor [6] by measuring impedance in electrolyte solutions with low conductivity.…”

Effect of enzyme location on activity and stability of trypsin and urease immobilized on porous membranes by using layer-by-layer self-assembly of polyelectrolyte

“…It has been found that the presence of amino groups facilitated the immobilization of URE onto the composite membrane. Disawal et al [22] have used LbL self-assembly to build multicomponent thin films containing two polyions, poly(dimethyl-diallyl ammonium chloride and Na-poly(styrene sulfonate) and two enzymes, URE and arginase, on gold electrode resonator. The influences of position and number of enzyme layers on the catalytic activity were investigated.…”

Proteinadsorption an festen Grenzflächen. Erwünscht und unerwünscht

“…Mit Hilfe einer solchen Oberflächenbeschichtung ist es gelungen, einen kommerziell erhältlichen Ammoniaksensor in einen Argininsensor umzufunktionieren, der die Aminosäure Arginin in physiologischen Konzentrationen detektieren kann [16]. Die Beispiele verdeutlichen, dass Protein-Polyelektrolyt-Multischichten ein enormes Anwendungspotenzial für die medizinische Analytik und die Biotechnologie besitzen.…”