Two types of mouse helper T cell clone. III. Further differences in lymphokine synthesis between Th1 and Th2 clones revealed by RNA hybridization, functionally monospecific bioassays, and monoclonal antibodies.

Abstract

Lymphokine synthesis patterns of a panel of 19 T cell clones have been evaluated, using mRNA hybridization methods to examine 11 different mRNAs induced by Con A. The two types of CD4+ Th cell clone described previously were clearly distinguished by this procedure, and the differences between the two types have now been extended to six induced products. With minor exceptions, only Th1 clones synthesized mRNA for IL-2, IFN-gamma, and lymphotoxin, and only Th2 clones synthesized mRNA for IL-4, IL-5, and another induced gene, P600. Four more induced products were expressed preferentially but not uniquely by one or another type of clone: mRNAs for GM-CSF, TNF, and another induced, secreted product (TY5) were produced in larger amounts by Th1 clones, whereas preproenkephalin was preferentially expressed by Th2 clones. IL-3 was produced in similar amounts by both types of clone. mAbs were used to establish three bioassays that were functionally monospecific for IL-2, IL-3, and IL-4, and a new anti-IFN gamma mAb, XMG1.2, was used to establish an ELISA for IFN-gamma. These four assays were used to show that secreted protein and mRNA levels correlated well for all cell lines. The implications of these findings for normal T cells are discussed.

CD8 T cell priming by B lymphocytes is CD4 help dependent

“…We show that T cell help for CD8 T cell activation by antigen-presenting B lymphocytes can also be transferred with the supernatant of antigen activated CD4 T cells, a fact reminiscent of experiments in which T cell help for antibody responses in vitro could be replaced by the supernatant of activated CD4 T cells [29], or a combination of IL-2 and IFN-c [30] or IL-4 and IL-5 [31]. While these studies emphasized the role of cytokines secreted by CD4 T cells in helping antibody responses by B lymphocytes, there is no information about a similar helper role of culture supernatants, or individual cytokines, in MHC class I-restricted activation of CD8 T cells by antigen-presenting B lymphocytes.…”

Host Responses and Antigens Involved in Protective Immunity to Mycobacterium tuberculosis

“…51). In the current model the CD4 cell subset is subdivided into counter-regulatory Th1 and Th2 cells, each producing a characteristic panel of cytokines [52,53]. This division was originally based on data from murine T cell clones, but data on cytokine profiles in human patients with different clinical forms of parasitic disease indicate that a similar division of human CD4 cells exist [54].…”

Experimental T cell-mediated inflammatory reactions in the murine oral mucosa. II. Immunohistochemical characterization of resident and infiltrating cells

“…MoAbs specifying the following cell surface markers were employed as primary reagents: CD11b [7] (Seralab, Crawley Down, UK), CD4 [8] and CD8 [9] (Becton Dickinson, Moutain View, CA), CD5 [10], CD25 [11] and CD45R (B220) (all from Pharmingen, San Diego, CA), and biotinylated mouse MoAbs to non-polymorphic murine MHC class II (clone 13/4) [12]. Rat MoAb to murine IL-2 (clone S4B6) [13] and interferon-gamma (IFN-) (clone XMG1.2) [14], gifts from Dr T. Mossman (Calgary, Canada), were used to detect the corresponding cytokine-containing cells. Following incubation for 60 min with primary MoAb, the sections were washed and exposed for 30 min to biotinylated rabbit IgG anti-rat immunoglobulin (Vector Labs Inc., Burlingame, CA) appropriately diluted in PBS containing 1% bovine serum albumin (BSA).…”