Two-wavelength Raman imaging for non-intrusive monitoring of transient temperature in microfluidic devices

Kuriyama, Reiko; Sato, Yohei

doi:10.1088/0957-0233/25/9/095203

Cited by 10 publications

(15 citation statements)

References 55 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The typical value for the standard deviation was 0.120 at 296 K, which corresponds to 9.1 K in temperature according to the slope of the calibration curve (−0.0133 K -1 ). In our previous study (Kuriyama and Sato, 2014), the calibration result showed a linear relationship between Raman intensity and temperature in a similar way, whereas the typical standard deviation of the intensity ratio was relatively small (equivalent to 1.5 K) compared to the present study due to the long exposure time (500 ms) and temporal averaging process over 30 successive Raman images. This result indicates that the variation in intensity ratio leads to a considerably large temperature error within the measurement area especially when the fast image acquisition is required.…”

Section: Results and Discussion 41 Calibration Experimentssupporting

confidence: 79%

“…This is similar in concept to Takahashi's methodology but it measures temperature (instead of concentration) for velocity determination. We assume that a pulsed heating is given in an upstream region of a channel flow and that the time-series temperature is probed in the downstream area using two-wavelength Raman imaging, which was proposed for non-intrusive temperature visualization (Kuriyama and Sato, 2014). If the temperature rise by the pulsed heating is successfully detected by Raman imaging, fluid velocity (v) can be obtained from the displacement of the peak temperature (∆x) and the time interval (∆t), i.e., v = ∆x/∆t.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

An investigation of measurement condition for non-intrusive velocity determination based on thermal tracing by Raman imaging

Kuriyama

Sato

2014

JTST

Self Cite

View full text Add to dashboard Cite

The present study proposes a velocity measurement based on thermal tracing by Raman imaging and investigates its applicability focusing on the error in temperature measurement, towards the establishment of a non-intrusive and micro-scale velocimetry. In order to realize fluorescence-free measurement, two-wavelength Raman imaging was employed to measure the temperature field in a channel flow. This technique exploits the contrasting temperature dependencies of hydrogen-bonded (HB) and non-hydrogen-bonded (NHB) OH stretching Raman bands of liquid water, and enables the determination of planar temperature distributions from the intensity ratio of the HB to NHB images. A calibration experiment showed a linear relationship between the temperature and the Raman intensity ratio in the range 293−333 K with temperature sensitivity of −0.56% K -1 .It was also confirmed that the spatial variation of the intensity ratio led to a large measurement error (approximately 9.1 K). Afterwards Raman images were acquired with various measurement conditions, and the influence of each parameter on the measurement error was quantitatively investigated. The apparent temperature variance was considerably reduced by increasing electron-multiplying gain and binning factor for spatial averaging, whereas an increase in the size of the measurement area resulted in a quadratic increase in the temperature variance due to the inhomogeneous excitation intensity. Finally, the requirements of the thermal flow conditions for the present methodology to be applied were quantitatively examined according to the measurement results.

show abstract

Section: Results and Discussion 41 Calibration Experimentssupporting

confidence: 79%

Section: Introductionmentioning

confidence: 99%

An investigation of measurement condition for non-intrusive velocity determination based on thermal tracing by Raman imaging

Kuriyama

Sato

2014

JTST

Self Cite

View full text Add to dashboard Cite

show abstract

“…In this study, we propose a label‐free method for evaluating and visualizing both intracellular and extracellular temperatures simultaneously using the Raman imaging of water inside a cell. Raman microscopy is one of the most powerful methods for observing living systems, and we pay attention to the fact the shape of the O−H stretching Raman band of water changes in accordance with temperature . We expected that a calibration curve obtained from the shape of the O−H stretching band and the temperature at each intracellular or extracellular region would enable temperature measurements of various regions.…”

Section: Figurementioning

confidence: 99%

Label‐Free Imaging of Intracellular Temperature by Using the O−H Stretching Raman Band of Water

2020

View full text Add to dashboard Cite

We propose a label-free method for measuring intracellular temperature using a Raman image of a cell in the OÀH stretching band. Raman spectra of cultured cells and the medium were first measured at various temperatures using a Raman microscope and the intensity ratio of the two regions of the O À H stretching band was calculated. The intensity ratio varies linearly with temperature in both the medium and cells, and the resulting calibration lines allow simultaneous visualization of both intracellular and extracellular temperatures in a label-free manner. We applied this method to the measurement of temperature changes after the introduction of FCCP (carbonyl cyanide-p-trifluoromethoxyphenylhydrazone) in living cells. We observed a temperature rise in the cytoplasm and succeeded in obtaining an image of the change in intracellular temperature after the FCCP treatment.Intracellular temperature is an essential parameter for understanding physiological phenomena, since all physiological functions involve the generation or absorption of heat. Cellular events such as respiration and cell division have now been examined and understood from the viewpoint of temperature change. The mechanism by which cells detect extracellular temperature [1,2] and transmit it to cell temperature homeostasis has also been studied extensively. [3] Sensing of intracellular temperature is applicable for the diagnosis of diseases, elucidation of disease onset mechanisms, and development of new target drugs.In recent years, the measurement of intracellular temperature has become one of the main topics of chemistry and biophysics, triggered in part by the development of various thermosensitive fluorescent dye molecules. [4][5][6][7][8][9][10][11] These dyes exhibit marked changes in their fluorescence intensity and/or fluorescence lifetime in response to the surrounding temperature, which can be used for evaluating and visualizing the temperature inside a cell. For example, temperature differences among cellular components [4] and heat production in the endoplasmic reticulum by Ca ions [7] have been reported using appropriate thermosensitive fluorescent dyes. However, temperature measurements using exogenous fluorescent dyes have disadvantages, such as the need for pretreatment for dye staining and changes in the intracellular environment with the introduction of dyes. Photobleaching of stained dyes increases the uncertainty of the evaluated temperature. It should be noted that the fluorescence intensity (lifetime) of these dyes depends not only on the surrounding temperature but also on other environmental factors such as ion concentration, viscosity and interaction with biological molecules. Therefore, careful attention must be paid to the estimation of intracellular temperature by fluorescence, [12] since the intracellular environment varies depending on the state of the cell. Furthermore, it is difficult to measure the temperature outside the region where fluorescent molecules are localized. The temperature of the surrounding medium cannot...

show abstract

Section: Figurementioning

confidence: 99%

Label‐Free Imaging of Intracellular Temperature by Using the O−H Stretching Raman Band of Water

2020

View full text Add to dashboard Cite

We propose a label‐free method for measuring intracellular temperature using a Raman image of a cell in the O−H stretching band. Raman spectra of cultured cells and the medium were first measured at various temperatures using a Raman microscope and the intensity ratio of the two regions of the O−H stretching band was calculated. The intensity ratio varies linearly with temperature in both the medium and cells, and the resulting calibration lines allow simultaneous visualization of both intracellular and extracellular temperatures in a label‐free manner. We applied this method to the measurement of temperature changes after the introduction of FCCP (carbonyl cyanide‐p‐trifluoromethoxyphenylhydrazone) in living cells. We observed a temperature rise in the cytoplasm and succeeded in obtaining an image of the change in intracellular temperature after the FCCP treatment.

show abstract

Two-wavelength Raman imaging for non-intrusive monitoring of transient temperature in microfluidic devices

Cited by 10 publications

References 55 publications

An investigation of measurement condition for non-intrusive velocity determination based on thermal tracing by Raman imaging

An investigation of measurement condition for non-intrusive velocity determination based on thermal tracing by Raman imaging

Label‐Free Imaging of Intracellular Temperature by Using the O−H Stretching Raman Band of Water

Label‐Free Imaging of Intracellular Temperature by Using the O−H Stretching Raman Band of Water

Contact Info

Product

Resources

About