Two‐way trafficking of Annexin V positive cells between mother and fetus: determination of apoptosis at delivery

Abstract

The study demonstrates the existence of a bidirectional transfer of fetal and maternal cells under apoptosis across the placenta and provides useful information regarding use of UCB for transplantation.

SHORT COMMUNICATION: Development of a Human Model to Study Homing Behavior of Immune Cells into Decidua and Placental Villi Under Ex Vivo Conditions

“…Cell trafficking or homing between mother and fetus in humans can be investigated only after delivery, by using maternal or umbilical blood and placental tissue. 14 In this study, we demonstrated that an ex vivo model of human placental perfusion with autologous maternal immune cells will be useful to investigate cell trafficking, homing and recruitment from the maternal circulation into decidual tissue and the fetal compartment.…”

Tissue factor‐dependent blood coagulation is enhanced following delivery irrespective of the mode of delivery

“…This protein, later determined to be annexin A5, is expressed on the cytoplasmic membranes of syncytiotrophoblast, where it is thought to act as an endogenous anticoagulant that regulates intervillous blood fluidity [15,21]. It also downregulates surface expression of TF [22] on apoptotic particles, including fetal cells that traffic to the maternal circulation [23]. In the first 12 h after delivery, these cells are largely removed from the maternal circulation [24].…”

Situación actual del diagnóstico prenatal no invasivo

“…Recientemente, Hyoda et al 17 han descrito la presencia de eritroblastos fetales en sangre materna hasta el momento del parto, así como su rápida desaparición después del nacimiento. La apoptosis sería la principal causa de la eliminación de las células fetales tras el parto; su persistencia en la sangre materna, una vez concluido el embarazo, se ha atribuido a progenitores resistentes a la apoptosis o a una regulación deficiente de ésta 18 .…”