Type and cellular location of reactive oxygen species determine activation or suppression of programmed cell death in Arabidopsis suspension cultures

Doyle, Siamsa M.; McCabe, Paul F.

doi:10.4161/psb.5.4.11399

Cited by 16 publications

(11 citation statements)

References 20 publications

(22 reference statements)

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“…To restrict oxidative stress and prevent PCD, one of the main strategies is to reduce ROS formation by a very tight control of the antioxidant systems and up regulation of different antioxidants, both enzymatic and non-enzymatic. This was supported by the observation that the exogenous application of nonspecific antioxidants temporarily suppresses PCD (Doyle and McCabe 2010). We also provide evidence that increasing the NO level by SNP reduced the Cu-induced ROS production and cell death in the basal region of the elongation zone and the meristem cells of hulless barley roots, but its reduction through c-PTIO caused a marked increase in ROS accumulation and cell death in Cd-treated roots.…”

Section: Discussionsupporting

confidence: 53%

Early generation of nitric oxide contributes to copper tolerance through reducing oxidative stress and cell death in hulless barley roots

2016

J Plant Res

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The objective of this study was to investigate the specific role of nitric oxide (NO) in the early response of hulless barley roots to copper (Cu) stress. We used the fluorescent probe diaminofluorescein-FM diacetate to establish NO localization, and hydrogen peroxide (H2O2)-special labeling and histochemical procedures for the detection of reactive oxygen species (ROS) in the root apex. An early production of NO was observed in Cu-treated root tips of hulless barley, but the detection of NO levels was decreased by supplementation with a NO scavenger, 2-phenyl-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide (c-PTIO). Application of sodium nitroprusside (a NO donor) relieved Cu-induced root inhibition, ROS accumulation and oxidative damage, while c-PTIO treatment had a synergistic effect with Cu and further enhanced ROS levels and oxidative stress. In addition, the Cu-dependent increase in activities of superoxide dismutase, peroxidase and ascorbate peroxidase were further enhanced by exogenous NO, but application of c-PTIO decreased the activities of catalase and ascorbate peroxidase in Cu-treated roots. Subsequently, cell death was observed in root tips and was identified as a type of programed cell death (PCD) by terminal deoxynucleotidyl transferase dUTP nick end labeling assay. The addition of NO prevented the increase of cell death in root tips, whereas inhibiting NO accumulation further increased the number of cells undergoing PCD. These results revealed that NO production is an early response of hulless barley roots to Cu stress and that NO contributes to Cu tolerance in hulless barley possibly by modulating antioxidant defense, subsequently reducing oxidative stress and PCD in root tips.

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Section: Discussionsupporting

confidence: 53%

Early generation of nitric oxide contributes to copper tolerance through reducing oxidative stress and cell death in hulless barley roots

2016

J Plant Res

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“…Most, if not all, stress responses disturb cell redox homeostasis and lead to oxidative stress by the accumulation of reactive oxygen species (ROS). Antioxidants and antioxidative enzymes are induced thereafter and they together with ROS provide systemic redox signaling throughout the whole plant [31]. In this study, except for the enhanced GSH biosynthesis pathway, Se treatments also induced antioxidative enzymes 1-Cys peroxiredoxin (spot U5 S ) in shoot and putative 1-Cys peroxiredoxin (spot U9 R ) in root.…”

mentioning

confidence: 83%

Proteomics analysis reveals multiple regulatory mechanisms in response to selenium in rice

Wang

Wong

2012

Journal of Proteomics

115

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“…On the other hand, catalase that specifically detoxifies H 2 O 2 suppressed PCD. These finding suggest that H 2 O 2 functions as PCD-inducing signal, but other types of ROS might act as negative regulator of PCD during heat stress (Doyle and McCabe 2010). Furthermore, MPK6 was found to be a component of PCD under heat stress.…”

Section: Programmed Cell Death Regulated By Ros Under Abiotic Stressmentioning

confidence: 67%

ROS as Key Players of Abiotic Stress Responses in Plants

Suzuki

2015

Reactive Oxygen Species and Oxidative Damage in Plants Under Stress

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Type and cellular location of reactive oxygen species determine activation or suppression of programmed cell death in Arabidopsis suspension cultures

Cited by 16 publications

References 20 publications

Early generation of nitric oxide contributes to copper tolerance through reducing oxidative stress and cell death in hulless barley roots

Early generation of nitric oxide contributes to copper tolerance through reducing oxidative stress and cell death in hulless barley roots

Proteomics analysis reveals multiple regulatory mechanisms in response to selenium in rice

ROS as Key Players of Abiotic Stress Responses in Plants

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