Type and strain dependence of enterovirus adsorption to activated sludge, soils and estuarine sediments

Gerba, Charles P.; Goyal, Sagar M.; Hurst, Christon J.; LaBelle, R L

doi:10.1016/0043-1354(80)90176-1

Cited by 41 publications

(28 citation statements)

References 6 publications

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“…Enteric viruses are known to be associated mainly with particles in environmental waters (40). Various environmental materials can capture human enteric viruses, including activated sludge (41), estuarine sediment (41,42), river sediment (43), and soils (41,44). da Silva et al showed that the GI and GII noroviruses found in wastewater samples were associated mainly with particles measuring Ͻ180 m (45).…”

Section: Discussionmentioning

confidence: 99%

“…This value is comparable with that obtained for the interactions between ß-galactose-specific lectins and lactose-carrying glycopolystyrenes, which have binding constants of 10 6 to 10 7 M Ϫ1 (54). The interactions between enteric viruses and environmental adsorbents were previously shown to have no binding specificity (41)(42)(43)(44)(45)47), whereas HuNoV binding to bacterial HBGAs is regarded as specific, as illustrated by human HBGAs and HuNoVs (12). In addition to enthalpy-driven adsorption via an array of hydrogen binding routes (28), the entropy effect caused by multiple binding sites also contributes to the increased binding avidity although structural analyses of bacterial EPS are essential to obtain an insight into the true value of the binding constant.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Histo-Blood Group Antigen-Like Substances of Human Enteric Bacteria as Specific Adsorbents for Human Noroviruses

Miura

Sano

Suenaga

et al. 2013

J Virol

170

183

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bHisto-blood group antigens (HBGAs) have been suggested to be receptors or coreceptors for human noroviruses (HuNoVs) expressed on the intestinal epithelium. We isolated an enteric bacterium strain (SENG-6), closely related to Enterobacter cloacae, bearing HBGA-like substances from a fecal sample of a healthy individual by using a biopanning technique with anti-HBGA antibodies. The binding capacities of four genotypes of norovirus-like particles (NoVLPs) to Enterobacter sp. SENG-6 cells were confirmed by enzyme-linked immunosorbent assay (ELISA). Transmission electron microscopy demonstrated that NoVLPs bound mainly to extracellular polymeric substances (EPS) of Enterobacter sp. SENG-6, where the HBGA-like substances were localized. EPS that contained HBGA-like substances extracted from Enterobacter sp. SENG-6 was shown by enzyme-linked immunosorbent assay (ELISA) to be capable of binding to NoVLPs of a GI.1 wild-type strain (8fIIa) and a GII.6 strain that can recognize A antigen but not to an NoVLP GI.1 mutant strain (W375A) that loses the ability to bind to A antigen. Enzymatic cleavage of terminal N-acetyl-galactosamine residues in the bacterial EPS weakened bacterial EPS binding to the GI.1 wild-type strain (8fIIa). These results indicate that A-like substances in the bacterial EPS play a key role in binding to NoVLPs. Since the specific binding of HuNoVs to HBGA-positive enteric bacteria is likely to affect the transmission and infection processes of HuNoVs in their hosts and in the environment, further studies of human enteric bacteria and their binding capacity to HuNoVs will provide a new scientific platform for understanding interactions between two types of microbes that were previously regarded as biologically unrelated.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Histo-Blood Group Antigen-Like Substances of Human Enteric Bacteria as Specific Adsorbents for Human Noroviruses

Miura

Sano

Suenaga

et al. 2013

J Virol

170

183

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“…Use as viral indicator Kott et al (1974) Chlorination Stagg et al (1977, Barbeau et al (2004), Coronell and Marinas (2004) Adsorption on particles Stagg et al (1977), Bixby and O'Brien (1979), Atherton and Bell (1983), Sakoda et al (1997), Dowd et al (1998) Moore et al (1975), Babich and Stotzky (1980), Sobsey et al (1980), , Schiffenbauer and Stotky (1982), Lipson and Stotzky (1983), Lipson and Stotzky (1984), Lipson and Stotzky (1985), Ohgaki and Mongkonsiri (1990), Sakoda et al (1997), Meschke and Sobsey (1998), Barbeau et al (2004) Montmorillonite Bitton et al (1972, Bitton and Mitchell (1974), Schaub and Sagik (1975), Babich and Stotzky (1980), Moore et al (1981), Taylor et al (1981), Schiffenbauer and Stotzky (1982), Lipson and Stotzky (1983), Vilker et al (1983), Lipson and Stotzky (1984), Lipson and Stotzky (1985), Dry dog food Moore et al (1975), Schaub and Sagik (1975) Wastewater solids, sludge Moore et al (1975), Gerba et al (1980), Ohgaki and M...…”

Section: Phagementioning

confidence: 99%

“…Gerba et al (1980) considered poliovirus 1, echovirus 1, 7, 29, and coxsackievirus B3 and B4 and their interactions with activated sludge, estuarine sediment, and sandy loam soil. The greatest variability in adsorption of the different viruses was seen in their behavior toward the soil samples and the least with the estuarine sediments.…”

Section: B Case Studies Of the Occurrence Of Particle-associated Virmentioning

confidence: 99%

Particle-Associated Viruses in Water: Impacts on Disinfection Processes

Templeton

Andrews

Hofmann

2008

Critical Reviews in Environmental Science and Technology

101

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Studies have shown that viruses are frequently attached onto or enmeshed within suspended particles in natural water and wastewater and that this viral particle-association can impede disinfection processes in some circumstances. This article summarizes the current state of knowledge of the occurrence of particle-associated viruses in the aquatic environment and the reported subsequent impacts on disinfection processes. The intention is that this article will help guide future research and aid regulators attempting to address the disinfection of particle-laden waters.

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“…On the other hand, the activated sludge process in wastewater treatment has been well known for removing viruses (9,23), though the removal efficiency depends on the species and type of viruses (10). Many pathogenic viruses, especially enteroviruses, have been observed to be easily captured by sewage sludge (10) and difficult to be eluted (29).…”

mentioning

confidence: 99%

Virus-Binding Proteins Recovered from Bacterial Culture Derived from Activated Sludge by Affinity Chromatography Assay Using a Viral Capsid Peptide

Sano

Matsuo

Omura

2004

Appl Environ Microbiol

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The contamination of water environments by pathogenic viruses has raised concerns about outbreaks of viral infectious diseases in our society. Because conventional water and wastewater treatment systems are not effective enough to inactivate or remove pathogenic viruses, a new technology for virus removal needs to be developed. In this study, the virus-binding proteins (VBPs) in a bacterial culture derived from activated sludge were successfully recovered. The recovery of VBPs was achieved by applying extracted crude proteins from a bacterial culture to an affinity column in which a custom-made peptide of capsid protein from the poliovirus type 1 (PV1) Mahoney strain (H 2 N-DNPASTTNKDKL-COOH) was immobilized as a ligand. VBPs exhibited the ability to adsorb infectious particles of PV1 Sabin 1 as determined by enzyme-linked immunosorbent assay. The evaluation of surface charges of VBPs with ion-exchange chromatography found that a majority of VBP molecules had a net negative charge under the conditions of affinity chromatography. On the other hand, a calculated isoelectric point implied that the viral peptide in the affinity column was also charged negatively. As a result, the adsorption of the VBPs to the viral peptide in the affinity column occurred with a strong attractive force that was able to overcome the electrostatic repulsive force. Two-dimensional electrophoresis revealed that the isolated VBPs include a number of proteins, and their molecular masses were widely distributed but smaller than 100 kDa. Amino acid sequences of N termini of five VBPs were determined. Homology searches for the N termini against all protein sequences in the National Center for Biotechnology Information (NCBI) database showed that the isolated VBPs in this study were newly discovered proteins. These VBPs that originated with bacteria in activated sludge might be stable, because they are existing in the environment of wastewater treatments. Therefore, a virus removal technology utilizing VBPs as viral adsorbents can be developed, since it is possible to replicate VBPs by protein cloning techniques.

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Type and strain dependence of enterovirus adsorption to activated sludge, soils and estuarine sediments

Cited by 41 publications

References 6 publications

Histo-Blood Group Antigen-Like Substances of Human Enteric Bacteria as Specific Adsorbents for Human Noroviruses

Histo-Blood Group Antigen-Like Substances of Human Enteric Bacteria as Specific Adsorbents for Human Noroviruses

Particle-Associated Viruses in Water: Impacts on Disinfection Processes

Virus-Binding Proteins Recovered from Bacterial Culture Derived from Activated Sludge by Affinity Chromatography Assay Using a Viral Capsid Peptide

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