Type I Collagen

Henriksen, Kim; Karsdal, Morten

doi:10.1016/b978-0-12-809847-9.00001-5

Cited by 63 publications

(52 citation statements)

References 59 publications

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“…Overall, articular cartilage repair did not achieve the quality of normal hyaline cartilage, regardless of marrow stimulation technique. 21 , 29 Kaul et al, 21 in a study of 5 patients undergoing TKA at a mean 8.8 months after failed marrow stimulation procedures, reported that cartilage-specific stains of the repair tissue were reduced compared with normal articular cartilage, suggesting poor cartilage quality compared with normal, and the repair tissue always showed positive immunoreactivity for type II collagen (most common type of collagen in hyaline cartilage 17 ) and type X collagen (mainly expressed in hypertrophic chondrocytes 18 ) while only sometimes showing positive immunoreactivity for type I collagen (most abundant collagen, expressed in almost all connective tissues 19 ).…”

Section: Resultsmentioning

confidence: 99%

Microfracture Versus Drilling of Articular Cartilage Defects: A Systematic Review of the Basic Science Evidence

Kraeutler

Aliberti

Scillia

et al. 2020

Orthopaedic Journal of Sports Medicine

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Background: Microfracture (MFx) is one of the most common techniques used for the treatment of articular cartilage defects, although recently there has been a trend toward the use of drilling rather than MFx for the treatment of these defects. Purpose: To perform a systematic review of basic science studies to determine the effect of microfracture versus drilling for articular cartilage repair. Study Design: Systematic review. Methods: A systematic review was performed by searching PubMed, the Cochrane Library, and EMBASE to identify basic science studies comparing outcomes of MFx versus drilling. The search phrase used was microfracture AND (drilling OR microdrilling). Inclusion criteria were basic science studies that directly compared the effect of MFx versus drilling on subchondral bone, bone marrow stimulation, and cartilage regeneration. Results: A total of 7 studies met the inclusion criteria and were included in this systematic review. Of these, 4 studies were performed in rabbits, 1 study in sheep, and 2 studies in humans. All of the included studies investigated cartilage repair in the knee. In the animal studies, microfracture produced fractured and compacted bone and led to increased osteocyte necrosis compared with drilling. Deep drilling (6 mm) was superior to both shallow drilling (2 mm) and MFx in terms of increased subchondral hematoma with greater access to marrow stroma, improved cartilage repair, and increased mineralized bone. However, the overall quality of cartilage repair tissue was poor regardless of marrow stimulation technique. In 2 studies that investigated repair tissue after MFx and/or drilling in human patients with osteoarthritis and cartilage defects, the investigators found that cartilage repair tissue did not achieve the quality of normal hyaline articular cartilage. Conclusion: In the limited basic science studies that are available, deep drilling of cartilage defects in the knee resulted in improved biological features compared with MFx, including less damage to the subchondral bone and greater access to marrow stroma. Regardless of marrow stimulation technique, the overall quality of cartilage regeneration was poor and did not achieve the characteristics of native hyaline cartilage. Overall, there is a general lack of basic science literature comparing microfracture versus drilling for focal chondral defects.

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Section: Resultsmentioning

confidence: 99%

Microfracture Versus Drilling of Articular Cartilage Defects: A Systematic Review of the Basic Science Evidence

Kraeutler

Aliberti

Scillia

et al. 2020

Orthopaedic Journal of Sports Medicine

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“…[1,8]) renders challenges for producing a universal hydrogel to accurately mimic individual tissue microenvironments. As Collagen I (Col I) is the most abundant ECM protein in the majority of mammalian connective tissues [23], with exceptions in a couple of tissues such as cartilage (mainly Col II) [24] and basement membrane (Col IV dominates) [25], it has been widely used as an ECM substitute in cell cultures. The major drawbacks of using Col I as a substratum for tissue cultures are the lack of the structural and biochemical heterogeneities of tissue ECM, requirement of chemical crosslinking for higher stiffness (> 1 kPa), limited long-term stability [26,27], and temperature-sensitive variations in fibril formation [28], etc.…”

Section: Introductionmentioning

confidence: 99%

Distinct phenotypes of cancer cells on tissue matrix gel

Ruud

Hiscox

et al. 2020

Breast Cancer Res

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Background: Breast cancer cells invading the connective tissues outside the mammary lobule or duct immerse in a reservoir of extracellular matrix (ECM) that is structurally and biochemically distinct from that of their site of origin. The ECM is a spatial network of matrix proteins, which not only provide physical support but also serve as bioactive ligands to the cells. It becomes evident that the dimensional, mechanical, structural, and biochemical properties of ECM are all essential mediators of many cellular functions. To better understand breast cancer development and cancer cell biology in native tissue environment, various tissue-mimicking culture models such as hydrogel have been developed. Collagen I (Col I) and Matrigel are the most common hydrogels used in cancer research and have opened opportunities for addressing biological questions beyond the two-dimensional (2D) cell cultures. Yet, it remains unclear whether these broadly used hydrogels can recapitulate the environmental properties of tissue ECM, and whether breast cancer cells grown on CoI I or Matrigel display similar phenotypes as they would on their native ECM. Methods: We investigated mammary epithelial cell phenotypes and metabolic profiles on animal breast ECMderived tissue matrix gel (TMG), Col I, and Matrigel. Atomic force microscopy (AFM), fluorescence microscopy, acini formation assay, differentiation experiments, spatial migration/invasion assays, proliferation assay, and nuclear magnetic resonance (NMR) spectroscopy were used to examine biological phenotypes and metabolic changes. Student's t test was applied for statistical analyses. Results: Our data showed that under a similar physiological stiffness, the three types of hydrogels exhibited distinct microstructures. Breast cancer cells grown on TMG displayed quite different morphologies, surface receptor expression, differentiation status, migration and invasion, and metabolic profiles compared to those cultured on Col I and Matrigel. Depleting lactate produced by glycolytic metabolism of cancer cells abolished the cell proliferation promoted by the non-tissue-specific hydrogel. Conclusion: The full ECM protein-based hydrogel system may serve as a biologically relevant model system to study tissue-and disease-specific pathological questions. This work provides insights into tissue matrix regulation of cancer cell biomarker expression and identification of novel therapeutic targets for the treatment of human cancers based on tissue-specific disease modeling.

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“…It is the predominant component of interstitial membranes. It is also associated with brosis and brogenesis (14). Hayashi et al, (2011) explained the excess deposition of type 1 collagen occurs in the maturation phase of scar formation, making the brils become large with stiff bands.…”

Section: Discussionmentioning

confidence: 99%

The impact of COL1A1 and COL6A1 expressions on hypospadias and penile curvature severity

Yuri

Gunadi

Lestari

et al. 2020

Preprint

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Background : Hypospadias, the most frequent congenital male external genitalia abnormality, is usually associated with curvature of the ventral penis, i.e. chordee. Abnormality of dartos tissue has been suggested as the pathophysiology of chordee. Collagen is one of the most abundant fibrous proteins within the extracellular matrix. In this study, we determined the expressions of collagen 1 (COL1A1) and COL6A1 in patients with hypospadias and associated them with the severity of penile curvature. Methods : We involved 60 children <18 years old, consisting of 20 distal hypospadias, 20 proximal hypospadias patients, and 20 controls in our institution from 2017 – 2020. The expressions of COL1A1 and COL6A1 from dartos tissue were determined by reverse-transcriptase polymerase chain reaction (qPCR). The penile curvature severity was classified as mild (<30 degree), moderate (30-60 degree), and severe (>60 degree). Results : qPCR showed that the COL1A1 and COL6A1 expressions were significantly downregulated in distal (0.88 (0.38-2.53) and 0.54 (0.16-4.35), respectively) and proximal 0.76 (0.33-2.57) and 0.57 (0.18-1.38), respectively) hypospadias groups compared to controls (1.85 (0.24-4.61) and 0.93 (0.17-4.06), respectively) with p -values of 0.024 and 0.018, respectively. Furthermore, there was a moderate correlation between COL1A1 and COL6A1 expressions ( r= 0.458, p< 0.0001). Interestingly, the COL1A1 and COL6A1 were also significantly downregulated in the moderate and severe compared to the mild chordee groups with p -values of 0.003 and 0.037, respectively. Conclusions : Aberrant COL1A1 and COL6A1 expressions might affect the abnormality of dartos tissue and penile curvature severity in hypospadias patients.

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Type I Collagen

Cited by 63 publications

References 59 publications

Microfracture Versus Drilling of Articular Cartilage Defects: A Systematic Review of the Basic Science Evidence

Microfracture Versus Drilling of Articular Cartilage Defects: A Systematic Review of the Basic Science Evidence

Distinct phenotypes of cancer cells on tissue matrix gel

The impact of COL1A1 and COL6A1 expressions on hypospadias and penile curvature severity

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