Type II Alveolar Pneumonocytes in Vitro

Douglas, William H.; McAteer, James A.; Smith, James R.; Braunschweiger, Walter R.

doi:10.1016/s0074-7696(08)60612-9

Cited by 18 publications

(8 citation statements)

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“…Type I1 cells isolated by one of the above procedures retain their morphological and functional properties in primary in vitro cultures reasonably well for -3-5 days, after which they progressively deteriorate (14,57,58,172). This deterioration is a difficulty with differentiated cells from adults.…”

Section: Alveolar Lining Cells: Type I Pneumocytesmentioning

confidence: 97%

“…Fetal lung cells are dissociated enzymatically; under suitable organotypic culture conditions they reaggregate to form histotypic elements resembling epithelial tubes in the fetal lung (58,59). In such cultures alveolarlike structures are formed that are lined by cells resembling type I1 cells and by attenuated cells similar to type I cells (58,59). These cultures perform the metabolic functions of surfactant biosynthesis and secrete the product into the internal alveolar spaces where it is transformed to tubular myelin.…”

Section: Alveolar Lining Cells: Type I Pneumocytesmentioning

confidence: 99%

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Lung Cell Biology

Weibel

1985

Comprehensive Physiology

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Section: Alveolar Lining Cells: Type I Pneumocytesmentioning

confidence: 97%

Section: Alveolar Lining Cells: Type I Pneumocytesmentioning

confidence: 99%

Lung Cell Biology

Weibel

1985

Comprehensive Physiology

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“…1977;Rose and Yajima, 1978;De Buysscher and Mendicino, 1978;Douglas et al, 1979;Tanswell and Smith, 1979). These recent successes prompted our efforts to develop a short-term primary cell culture system enriched in mucin-producing cells derived from the tracheobronchial tree.…”

Section: Introductionmentioning

confidence: 99%

“…Methods are currently available to permit isolation and culture of a variety of epithelial cells that retain their differentiated functions in primary culture. Differentiated cell cultures were established initially by application of clonal culture techniques to enzymatically dispersed suspensions of chick embryo skeletal muscle (Konigsberg, 1963), and subsequently extended to keratinocytes (Rheinwald and Green, 1975), type I1 alveolar epithelial cells (Douglas e t al., 1976;Mason et al, 1977;Douglas et al, 1979;Tanswell and Smith, 1979). bronchial epithelial cells (Stoner et al, 1978), prostatic epithelial cells (Kaighn and Babcock.…”

mentioning

confidence: 99%

“…Due to the continued interest in the cell biology of the respiratory tract, many laboratories are devoting effort to the isolation and primary culture of cells from various regions of the airways and lung (Douglas et al, 1976;Boat et al, 1976;Rose and Yajima, 1977;Ryan and Ryan, 1977;Mason et al. 1977;Rose and Yajima, 1978;De Buysscher and Mendicino, 1978;Douglas et al, 1979;Tanswell and Smith, 1979). These recent successes prompted our efforts to develop a short-term primary cell culture system enriched in mucin-producing cells derived from the tracheobronchial tree.…”

mentioning

confidence: 99%

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Isolation, culture, and preliminary characterization of mucin‐producing cells from trachea of the domestic fowl

Douglas¹,

Gustafson²,

Aghajanian³

et al. 1982

Anat. Rec.

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Primary cell cultures enriched in mucin-producing cells and basal cells were established from the trachea of the domestic fowl. Epithelial cells were selectively removed from the trachea after incubation in 0.1% pronase/0.1% EDTA in Moscona's saline. The majority of the ciliated cells were removed during the initial 30 minutes of incubation. After 50 minutes of incubation, aggregates of mucin-producing cells and basal cells were removed in large numbers. The cellular aggregates rapidly attached to a collagen-coated substratum and the cells spread out on the culture surface. The mucin-producing cells retained their AB/PAS-reactive secretory granules. The basal cells replicated and as the culture approached confluency, these cells developed a fine dusting of AB/PAS-reactive material; later, larger secretory granules appeared in the cells. These observations suggest that mucin-producing cells are capable of retaining their AB/PAS-reactive secretory products in primary culture and that basal cells are capable of differentiating into mucin-producing cells in vitro.

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Hormones and the lung II. Immunohistochemical localization of thyroid hormone binding in type II pulmonary epithelial cells clonally‐derived from adult rat lung

et al. 1979

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The type II pulmonary epithelial cell is the recognized state of surfactant synthesis and storage. Results of recent studies indicate that the thyroid hormones, triiodothyronine (T3) and thyroxine (T4), may be important regulators of surfactant production and/or release. Direct and indirect immunofluorescence techniques were used in an attempt to demonstrate binding of T3 and T4 in monolayer cultures of isolated type II cells. These cultured epithelial cells are clonally-derived from adult rat lung, retain a diploid karyotype through 35 population doublings in vitro, contain granular inclusions (lamellar bodies) in the perinuclear cytoplasm, and synthesize phosphatidylcholine via the CDP-choline pathway. In isolated type II cells, either of two fluorescent patterns was observed: (a) nuclear fluorescence accompanied by a reticular perinuclear network; or (b) diffuse cytoplasmic accumulations with concentrations around perinculear cytoplasmic inclusions. Ultrastructurally these inclusions had the typical appearance of lamellar bodies. Histochemical studies demonstrated that these inclusions contained surfactant-associated nonspecific esterases and stained with Nile blized markers for pulmonary surfactant indicate that these inclusions are indeed lamellar bodies, the putative sites of surfactant synthesis and/or storage. These findings suggest that the type II pulmonary epithelial cell contains specific binding sites for thyroid hormones, and support the hypothesis that thyroid hormones are regulators of surfactant metabolism.

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Type II Alveolar Pneumonocytes in Vitro

Cited by 18 publications

References 50 publications

Lung Cell Biology

Lung Cell Biology

Isolation, culture, and preliminary characterization of mucin‐producing cells from trachea of the domestic fowl

Hormones and the lung II. Immunohistochemical localization of thyroid hormone binding in type II pulmonary epithelial cells clonally‐derived from adult rat lung

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