Type III Collagen Messenger RNA is Modulated in Non-Compliant Human Bladder Tissue

Kaplan, E. Paul; Richier, John C.; Howard, Pamela S.; Ewalt, David H.; Lin, Victor K.

doi:10.1016/s0022-5347(01)64782-7

Cited by 41 publications

(13 citation statements)

References 16 publications

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“…In the present study, we found that resveratrol significantly suppressed the proliferation of fibroblasts from 25 to 400 mM, and no cytotoxicity of resveratrol was observable at a dose of 75 mM. Previous reports indicated that 100 mM resveratrol did not induce cytotoxicity in rat-1 fibroblast cells, 42) but a decreased number of rat intestinal smooth muscle cells resulted from cellcycle arrest following treatment with 100 mM resveratrol at 24 h. 18) We speculate that cytotoxicity comes into play if resveratrol is used at a concentration of more than the 150 mM in vitro, because the fibroblasts treated with 150, 300, and 400 mM resveratrol for 24,48, and 72 h demonstrated swollen shapes or apoptotic and necrotic characteristics (Fig. 2).…”

Section: Discussionmentioning

confidence: 79%

“…The specific primers for the expression analysis of types I and III procollagen followed previously published articles. 24,25) They were synthesized by Shanghai Sangon Biotech Company, and are listed in Table 1. Amplification was performed under specific reaction conditions: 3 min initial denaturation at 94 C, 35 cycles of 94 C for 1 min, 55 C for 2 min, and 72 C for 2 min, and a final extension of 5 min at 72 C. Finally, 10 or 15 mL of PCR products for type I and III procollagen were separated by electrophoresis on 1.5% agarose gels and stained with ethidium bromide, and images were captured under UV illumination.…”

Section: Methodsmentioning

confidence: 99%

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Resveratrol-Mediated Reduction of Collagen by Inhibiting Proliferation and Producing Apoptosis in Human Hypertrophic Scar Fibroblasts

Zeng

Fang

Jin

et al. 2013

Bioscience, Biotechnology, and Biochemistry

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Section: Discussionmentioning

confidence: 79%

Section: Methodsmentioning

confidence: 99%

Resveratrol-Mediated Reduction of Collagen by Inhibiting Proliferation and Producing Apoptosis in Human Hypertrophic Scar Fibroblasts

Zeng

Fang

Jin

et al. 2013

Bioscience, Biotechnology, and Biochemistry

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“…It has been demonstrated that the human non-compliant bladder is characterized histologically by an increased deposition of ECM protein, especially type III collagen, in the muscle wall (Kaplan et al 1997). Prolidase is a homodimeric iminodipeptidase that releases carboxy-terminal proline or hydroxyproline from oligopeptides.…”

Section: Discussionmentioning

confidence: 99%

Serum prolidase activity, oxidative stress, and nitric oxide levels in patients with bladder cancer

Geçit

Aslan

Güneş

et al. 2012

J Cancer Res Clin Oncol

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ObjectivesProlidase is a member of the matrix metalloproteinase family. It plays a major role in collagen turnover, matrix remodeling and cell growth. Nitric oxide (NO) regulates many processes such as collagen synthesis and matrix remodeling. Thus, NO may augment angiogenesis, tumor invasion, and metastasis. The aim of this study was to investigate total antioxidant status (TAS), malondialdehyde (MDA) and NO levels in patients with bladder cancer and to determine their relationship with prolidase activity.Design and methodsThirty-five patients with bladder cancer and 32 controls were enrolled. Serum TAS, MDA, prolidase activity and NO levels were determined.ResultsSerum prolidase activity, NO levels and MDA levels were significantly higher in bladder cancer than controls (all, P < 0.05), while TAS levels were significantly lower (P < 0.05).ConclusionsOur results show that increased prolidase seems to be associated with increased NO levels and oxidative stress along with decreased antioxidant levels in bladder cancer.

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“…This increased collagen content is at least in part produced by detrusor smooth muscle cells and is thought to be a major cause of decreased bladder compliance 6. However, it is not clear how elevated collagen composition affects total intravesical pressure in the setting of a neuropathic, spastic bladder.…”

mentioning

confidence: 99%

The Effect of Epigenetic Therapy on Congenital Neurogenic Bladders—A Pilot Study

Hodges

Yoo

Mishra

et al. 2010

Urology

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OBJECTIVES-To demonstrate that human smooth muscle cells derived from neurogenic bladders produce more collagen in vitro than smooth muscle cells derived from normal bladders, and that epigenetic therapy may normalize this increased collagen production.METHODS-Human smooth muscle cells from normal (n = 3) and neurogenic bladders (n = 3) were cultured in normal culture media and at different concentrations of the histone deacetylase inhibitors trichostatin A, valproic acid, and the DNA methylation inhibitor 5-azacytidine (5-aza). Collagen type I and III gene expression was measured using real-time quantitative reverse transcription-polymerase chain reaction after varying doses of drug exposure. Cell viability was measured using trypan blue. RESULTS-The smooth muscle cells from neurogenic bladders released significantly more collagen than the normal bladder cells (mean 4.1 vs 1.8 μg/mL in control media) when grown in normal conditions. Treatment with trichostatin A at 50 ng/mL decreased the collagen level in cells from neurogenic bladders to almost normal levels (2.1 μg/mL). In addition, valproic acid treatment decreased collagen types I and III gene expression relative to controls, with maximal effect at 300 mg/mL. These treatments had little effect on cell viability. CONCLUSIONS-Histone deacetylase inhibitors decreased collagen production of smooth muscle cells from neurogenic bladders in vitro. These agents may be a means of effectively preventing bladder fibrosis in patients with this condition.In children with myelodysplasia, a primary concern is the maintenance of low bladder storage pressure. Elevated intravesical pressure caused by a poorly compliant bladder in patients with spinal dysraphism is associated with damage to the upper urinary tract, and if not appropriately treated, renal failure. 1 Many treatment modalities have been developed to prevent or treat this disease process. One example is the use of chronic oral antimuscarinic therapy from an early age, coupled with clean intermittent catheterization. 2 Intravesical instillation of oxybutynin and other agents also has been attempted. 3,4 However, these therapies vary in efficacy and are associated with a number of side effects. Thus, development of novel treatment options is desirable.Neuropathic bladders have an increased ratio of type-III to type-I collagen, with an absolute increase in the amount of type-III collagen. 5 This increased collagen content is at least in part produced by detrusor smooth muscle cells and is thought to be a major cause of

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Type III Collagen Messenger RNA is Modulated in Non-Compliant Human Bladder Tissue

Abstract: Type III collagen mRNA levels are increased in the human non-compliant bladder. Therefore the accumulation of type III collagen protein is, in part, transcriptionally regulated.

Cited by 41 publications

References 16 publications

Resveratrol-Mediated Reduction of Collagen by Inhibiting Proliferation and Producing Apoptosis in Human Hypertrophic Scar Fibroblasts

Resveratrol-Mediated Reduction of Collagen by Inhibiting Proliferation and Producing Apoptosis in Human Hypertrophic Scar Fibroblasts

Serum prolidase activity, oxidative stress, and nitric oxide levels in patients with bladder cancer

The Effect of Epigenetic Therapy on Congenital Neurogenic Bladders—A Pilot Study

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